Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,106,131	T→C	S366G (AGC→GGC)	wbbH ←	putative O‑antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,106,131	0	T	C	100.0%	160.8 / NA	44	S366G (AGC→GGC)	wbbH	putative O‑antigen polymerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/22);  total (22/22)

CATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCA  >  NC_000913/2105994‑2106265                                                                                                                                          |                                                                                                                                       cATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCaa                                                                                                                                      <  1:471601/140‑1 (MQ=255)    cTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAatat                                                                                                                                   >  1:214578/1‑140 (MQ=255)    cTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAatat                                                                                                                                   >  1:80802/1‑140 (MQ=255)    cTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAatat                                                                                                                                   >  1:321282/1‑140 (MQ=255)     ttttCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATAtt                                                                                                                                  <  2:94302/140‑1 (MQ=255)            gCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATg                                                                                                                           <  1:69833/140‑1 (MQ=255)              tttAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGaa                                                                                                                         >  2:243924/1‑140 (MQ=255)              tttAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGaa                                                                                                                         <  2:214578/140‑1 (MQ=255)                       aGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATg                                                                                                                >  1:443695/1‑140 (MQ=255)                       aGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATg                                                                                                                >  1:92498/1‑140 (MQ=255)                            cgcgTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATaaa                                                                                                           >  1:516294/1‑140 (MQ=255)                              cgTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATaaaaa                                                                                                         >  1:58433/1‑140 (MQ=255)                                tCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAAt                                                                                                       <  2:516294/140‑1 (MQ=255)                                       aaaTTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATgaaagaa                                                                                                <  2:350399/140‑1 (MQ=255)                                       aaaTTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATgaaagaa                                                                                                <  2:321282/140‑1 (MQ=255)                                       aaaTTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATgaaagaa                                                                                                <  2:126315/140‑1 (MQ=255)                                       aaaTTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGACATGAAGCTTTCATGATAAAAAATgaaagaa                                                                                                <  2:315458/140‑1 (MQ=255)                                                     cAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTAtaaataaa                                                                                  >  2:105239/1‑140 (MQ=255)                                                               aTTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATaa                                                                        <  2:138876/140‑1 (MQ=255)                                                                    cACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAatat                                                                   >  1:433392/1‑140 (MQ=255)                                                                       tttATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATAtttt                                                                <  2:280773/140‑1 (MQ=255)                                                                         tATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTca                                                              <  1:130408/140‑1 (MQ=255)                                                                         tATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTca                                                              <  2:92498/140‑1 (MQ=255)                                                                         tATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTca                                                              <  2:443695/140‑1 (MQ=255)                                                                            tttCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAg                                                           <  2:289717/140‑1 (MQ=255)                                                                                     cTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAAt                                                  >  2:134579/1‑140 (MQ=255)                                                                                       tAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAAttt                                                >  2:262300/1‑140 (MQ=255)                                                                                       tAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAAttt                                                >  2:46132/1‑140 (MQ=255)                                                                                           aaaTTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCgag                                            <  2:147352/140‑1 (MQ=255)                                                                                                               tACAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCt                        >  1:286344/1‑140 (MQ=255)                                                                                                                aCAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTg                       <  2:82922/140‑1 (MQ=255)                                                                                                                 cAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAac                            <  1:227613/134‑1 (MQ=255)                                                                                                                 cAAAGAGTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAac                            >  2:227613/1‑134 (MQ=255)                                                                                                                    agagTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAAt                   >  2:270295/1‑140 (MQ=255)                                                                                                                    agagTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAAt                   >  2:88381/1‑140 (MQ=255)                                                                                                                    agagTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAAt                   >  2:70129/1‑140 (MQ=255)                                                                                                                     gagTTATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATa                  <  2:80802/140‑1 (MQ=255)                                                                                                                        ttATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAg               >  2:219176/1‑140 (MQ=255)                                                                                                                        ttATTTGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAg               <  1:46132/140‑1 (MQ=255)                                                                                                                           tttGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAac                            >  2:459388/1‑124 (MQ=255)                                                                                                                           tttGTATCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAac                            <  1:459388/124‑1 (MQ=255)                                                                                                                                aTCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAAt       >  2:262444/1‑140 (MQ=255)                                                                                                                                 tCCAACTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATa      >  2:315789/1‑140 (MQ=255)                                                                                                                                     aCTGCCAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACca  <  1:243924/140‑1 (MQ=255)                                                                                                                                     aCTGCCAATATTACTCATGAAGCTTTCATGATAGAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATATTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACca  >  2:216180/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCA  >  NC_000913/2105994‑2106265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑