Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	124,972	T→C	T652T (ACT→ACC)	aceE →	pyruvate dehydrogenase E1 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	124,972	0	T	C	100.0%	157.3 / NA	43	T652T (ACT→ACC)	aceE	pyruvate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/24);  total (19/24)

ATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTAC  >  NC_000913/124838‑125107                                                                                                                                       |                                                                                                                                        aTTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATccc                                                                                                                                    >  2:498988/1‑140 (MQ=255) aTTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATccc                                                                                                                                    <  2:330212/140‑1 (MQ=255)  ttGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCg                                                                                                                                   <  1:36286/140‑1 (MQ=255)    ggCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGaa                                                                                                                                 <  1:79583/140‑1 (MQ=255)          cTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTAt                                                                                                                           >  1:68716/1‑140 (MQ=255)           tgtgCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTAtc                                                                                                                          >  1:186300/1‑140 (MQ=255)                     ggcTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGAcc                                                                                                                >  1:153002/1‑140 (MQ=255)                       cTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCg                                                                                                              >  2:514880/1‑140 (MQ=255)                       cTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTACGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCCTCCCGAACTGTATCTCTTACGACCCg                                                                                                              >  1:312245/1‑140 (MQ=255)                          gCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGgct                                                                                                           <  2:68716/140‑1 (MQ=255)                                            gCTTCCTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCt                                                                                         <  1:219928/140‑1 (MQ=255)                                               tCCTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGtc                                                                                      <  2:186300/140‑1 (MQ=255)                                                ccTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGtca                                                                                     <  2:111192/140‑1 (MQ=255)                                                ccTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGtca                                                                                     <  1:22921/140‑1 (MQ=255)                                                     tCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATcatg                                                                                <  2:425515/140‑1 (MQ=255)                                                       ggcggTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATcatgca                                                                              >  2:482460/1‑140 (MQ=255)                                                         cggTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATcatgcatg                                                                            <  1:17949/140‑1 (MQ=255)                                                         cggTACTTCCGGTCGTACCACCATGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATcatgcatg                                                                            >  2:137892/1‑140 (MQ=255)                                                            tACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACg                                                                         <  1:482460/140‑1 (MQ=255)                                                                  cGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTgg                                                                   <  2:152982/140‑1 (MQ=255)                                                                   ggTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGa                                                                  >  2:45579/1‑140 (MQ=255)                                                                    gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAg                                                                 <  1:391352/140‑1 (MQ=255)                                                                        tACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTa                                                             >  1:538358/1‑140 (MQ=255)                                                                                 gAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTg                                                    <  1:514880/140‑1 (MQ=255)                                                                                  aaCGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGa                                                   >  2:495806/1‑140 (MQ=255)                                                                                    cGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTT‑CGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGaaaa                                                <  2:329128/140‑1 (MQ=255)                                                                                      gCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGaaaaa                                               <  1:137892/140‑1 (MQ=255)                                                                                            gTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAagag                                         >  1:231672/1‑140 (MQ=255)                                                                                             tCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAgaga                                        <  2:538358/140‑1 (MQ=255)                                                                                                      cGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTtact                               >  2:13197/1‑140 (MQ=255)                                                                                                      cGAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTtact                               >  2:401270/1‑140 (MQ=255)                                                                                                       gAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTtacta                              >  2:66468/1‑140 (MQ=255)                                                                                                       gAAGATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTtacta                              <  1:13197/140‑1 (MQ=255)                                                                                                          gATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTtactacta                           <  1:66468/140‑1 (MQ=255)                                                                                                          gATGGTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTtactacta                           <  1:498988/140‑1 (MQ=255)                                                                                                             ggTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAgaga                                        >  2:479785/1‑124 (MQ=255)                                                                                                             ggTCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAgaga                                        <  1:479785/124‑1 (MQ=255)                                                                                                               tCACAGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCa                      <  2:153002/140‑1 (MQ=255)                                                                                                                   aGCCACATTCAGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTAc                  >  2:27490/1‑140 (MQ=255)                                                                                                                             aGTCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGaa        >  1:417368/1‑140 (MQ=255)                                                                                                                               tCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACg          <  2:156789/136‑1 (MQ=255)                                                                                                                               tCGCTGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACg          >  1:156789/1‑136 (MQ=255)                                                                                                                                   tGACCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTAc  <  1:495806/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        ATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTGGCTTCCTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTAC  >  NC_000913/124838‑125107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑