Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,234,242	T→C	G70G (GGT→GGC)	preT →	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,234,242	0	T	C	100.0%	136.9 / NA	38	G70G (GGT→GGC)	preT	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/19);  total (19/19)

TGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAAC  >  NC_000913/2234108‑2234367                                                                                                                                       |                                                                                                                              tGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGt                                                                                                                          <  2:476708/140‑1 (MQ=255) tGTTTATTATGTCACGACGCTCCCTGTAGTAAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGt                                                                                                                          <  2:330903/140‑1 (MQ=255)       ttatGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCCATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGc                                                                                                                    <  2:376769/140‑1 (MQ=255)       ttatGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGc                                                                                                                    <  2:506953/140‑1 (MQ=255)       ttatGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGc                                                                                                                    <  2:379575/140‑1 (MQ=255)           gTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCaga                                                                                                                <  1:270397/140‑1 (MQ=255)             cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGc                                                                                                                    <  1:209759/134‑1 (MQ=255)             cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGc                                                                                                                    >  2:209759/1‑134 (MQ=255)             cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAgt                                                                                                              >  2:84863/1‑140 (MQ=255)             cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAgt                                                                                                              >  2:500258/1‑140 (MQ=255)             cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAgt                                                                                                              >  2:107983/1‑140 (MQ=255)             cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAgt                                                                                                              >  1:406378/1‑140 (MQ=255)                     tCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGa                                                                                                      <  1:500258/140‑1 (MQ=255)                               cAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAAtt                                                                                            >  1:487748/1‑140 (MQ=255)                                aGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTa                                                                                           >  2:494281/1‑140 (MQ=255)                                  gCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATg                                                                                         >  2:453560/1‑140 (MQ=255)                                   cTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGc                                                                                        <  1:107983/140‑1 (MQ=255)                                      gCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCaa                                                                                     >  1:35700/1‑140 (MQ=255)                                       cccGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCaaa                                                                                    <  2:29498/140‑1 (MQ=255)                                       cccGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCaaa                                                                                    >  2:414962/1‑140 (MQ=255)                                         cGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGc                                                                                  <  1:313101/140‑1 (MQ=255)                                                gACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTa                                                                           <  2:406378/140‑1 (MQ=255)                                                 aCCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTAc                                                                          >  2:165161/1‑140 (MQ=255)                                                 aCCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTAc                                                                          >  2:393217/1‑140 (MQ=255)                                                     aTCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGt                                                                      >  1:336093/1‑140 (MQ=255)                                                                    ttCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCt                                                       <  2:72306/140‑1 (MQ=255)                                                                     tCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTc                                                      >  1:235004/1‑140 (MQ=255)                                                                                      gTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGc                                     <  2:252178/140‑1 (MQ=255)                                                                                       tAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCt                                    >  1:372348/1‑140 (MQ=255)                                                                                       tAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCt                                    >  2:356471/1‑140 (MQ=255)                                                                                       tAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCt                                    >  2:79956/1‑140 (MQ=255)                                                                                         aTTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTa                                  <  2:35700/140‑1 (MQ=255)                                                                                                aGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGtt                           <  1:84863/140‑1 (MQ=255)                                                                                                            gaCAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGAtt               <  1:393217/140‑1 (MQ=255)                                                                                                                  tCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGaac         >  1:426550/1‑140 (MQ=255)                                                                                                                     cgAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGaa          >  2:53037/1‑136 (MQ=255)                                                                                                                     cgAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGaa          <  1:53037/136‑1 (MQ=255)                                                                                                                         aataatGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAAc  <  2:426271/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                              TGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAAC  >  NC_000913/2234108‑2234367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑