Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,337,694	T→C	D576G (GAC→GGC)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,337,694	0	T	C	88.9%	20.6 / ‑4.0	9	D576G (GAC→GGC)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (8/0);  total (9/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCA  >  NC_000913/2337558‑2337823                                                                                                                                         |                                                                                                                                  gTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGcctt                                                                                                                                <  1:300767/140‑1 (MQ=255)  ttGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGccttc                                                                                                                               <  1:532931/140‑1 (MQ=255)  ttGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGccttc                                                                                                                               <  2:259081/140‑1 (MQ=255)           ggACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAAt                                                                                                                      >  1:135921/1‑140 (MQ=255)                                         ggCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCacg                                                                                        >  2:358705/1‑140 (MQ=255)                                            aaCTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCacgacg                                                                                     >  2:66296/1‑140 (MQ=255)                                                 aTAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTgcg                                                                                >  2:470557/1‑140 (MQ=255)                                                         tCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTAt                                                                        >  1:16590/1‑140 (MQ=255)                                                                     cccgcccAGGGCTGGAAAACAACAAAATTGGGTCGTGAGGTTCCCCCCCCAACAGTCGGTCAAAAAACCCTTCTTCTTTAATCCGCGCGGCATATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGc                                                            <  2:285254/135‑1 (MQ=255)                                                                         aCCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGAt                                                        >  2:180866/1‑140 (MQ=255)                                                                                   gaaaaGCAAAGAATGTGGTCGGGGGTGTCCGCCCCCAGCAGTGGTTAAATAAACCTTTTTTTTTAAATACGTGGGGCAGTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTa                                              <  2:135921/137‑1 (MQ=255)                                                                                                              ttCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACcac                   >  1:112495/1‑140 (MQ=255)                                                                                                               tCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACcaca                  >  1:305332/1‑140 (MQ=255)                                                                                                                       agcagTCGGTCAAAAAAGCCTTCTTTTTTAATACGGGCGGAAGTTTTCCTTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTg          <  2:108275/140‑1 (MQ=255)                                                                                                                        gcagTCGGTCGATAAAGCTTTCTTCTTTAATGCGTGCGGCAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTgg         <  2:16590/140‑1 (MQ=255)                                                                                                                               gtcgATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCa  >  1:108275/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                  GTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCA  >  NC_000913/2337558‑2337823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑