Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,376,357	T→C	T155A (ACT→GCT)	menB ←	1,4‑dihydroxy‑2‑naphthoyl‑CoA synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,376,357	0	T	C	100.0%	110.1 / NA	31	T155A (ACT→GCT)	menB	1,4‑dihydroxy‑2‑naphthoyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/15);  total (16/15)

CCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGTCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAG  >  NC_000913/2376225‑2376490                                                                                                                                     |                                                                                                                                      ccATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCg                                                                                                                                >  1:131728/1‑140 (MQ=255)          gcgcCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCa                                                                                                                       >  1:271795/1‑140 (MQ=255)          gcgcCTGTTTTGCGTCCTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCa                                                                                                                       >  1:416786/1‑140 (MQ=255)            gcCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCAtt                                                                                                                     >  2:246743/1‑140 (MQ=255)                 ttttGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTg                                                                                                                >  2:375707/1‑140 (MQ=255)                 ttttGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTg                                                                                                                <  1:312599/140‑1 (MQ=255)                 ttttGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTg                                                                                                                <  2:197363/140‑1 (MQ=255)                 ttttGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTg                                                                                                                <  1:375707/140‑1 (MQ=255)                 ttttGCGTCCTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTg                                                                                                                <  2:416786/140‑1 (MQ=255)                      cgtcgtACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCcgcg                                                                                                           <  2:519107/140‑1 (MQ=255)                            aCTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTc                                                                                                     <  2:271795/140‑1 (MQ=255)                                   gCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGc                                                                                              >  1:159933/1‑140 (MQ=255)                                     acaGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGcac                                                                                            >  1:201098/1‑140 (MQ=255)                                                      cgcgCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCAtgtg                                                                                    <  1:407956/131‑1 (MQ=255)                                                      cgcgCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCAtgtg                                                                                    >  2:407956/1‑131 (MQ=255)                                                                tCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGAccgccgccg                                                                 <  1:48661/140‑1 (MQ=255)                                                                tCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGAccgccgccg                                                                 <  1:246743/140‑1 (MQ=255)                                                                    cccGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATgg                                                             >  2:145746/1‑140 (MQ=255)                                                                       gacgaTGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGt                                                          <  2:201098/140‑1 (MQ=255)                                                                                           aaGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACa                                      <  2:347528/140‑1 (MQ=255)                                                                                             gcgcCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAAc                                    >  1:441466/1‑140 (MQ=255)                                                                                               gcCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCg                                  >  2:336163/1‑140 (MQ=255)                                                                                                ccccAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGACATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCgg                                 >  2:18388/1‑140 (MQ=255)                                                                                                 cccAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGCCAACCGGt                                >  1:339429/1‑140 (MQ=255)                                                                                                    aGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTc                             >  2:238639/1‑140 (MQ=255)                                                                                                       cgccgTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGa                          <  1:336163/140‑1 (MQ=255)                                                                                                             cGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTa                    <  1:238639/140‑1 (MQ=255)                                                                                                                            tCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTgg     <  2:159933/140‑1 (MQ=255)                                                                                                                             cGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGGGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTa                    <  2:275831/124‑1 (MQ=255)                                                                                                                             cGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTa                    >  1:275831/1‑124 (MQ=255)                                                                                                                               ggCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAg  >  2:185525/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      CCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGTCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAG  >  NC_000913/2376225‑2376490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑