Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,387,282	C→T	L117F (CTC→TTC)	yfbM →	DUF1877 domain‑containing protein YfbM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,387,282	0	C	T	100.0%	121.8 / NA	35	L117F (CTC→TTC)	yfbM	DUF1877 domain‑containing protein YfbM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/15);  total (20/15)

CTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCG  >  NC_000913/2387147‑2387416                                                                                                                                        |                                                                                                                                       cTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCaa                                                                                                                                    >  1:20112/1‑140 (MQ=255)     gTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGaa                                                                                                                                <  1:439939/140‑1 (MQ=255)        aaCACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCTACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATg                                                                                                                             <  2:159618/140‑1 (MQ=255)               ccTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGAttt                                                                                                                      >  2:458637/1‑140 (MQ=255)                            aTAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGAc                                                                                                         <  1:315181/140‑1 (MQ=255)                            aTAGATGGATACCTTGGATTGGCCTGGCAGCGGGAGTTAGGCGCGACAATCGGCCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAGATGGAGATTTATCCGGGAGTgcc                                                                                                         <  2:441210/140‑3 (MQ=255)                                    cTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCag                                                                                                 <  2:195580/140‑1 (MQ=255)                                    cTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCag                                                                                                 >  1:401598/1‑140 (MQ=255)                                    cTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCag                                                                                                 <  1:76624/140‑1 (MQ=255)                                             aTTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTag                                                                                        <  2:401598/140‑1 (MQ=255)                                                 aCCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAggg                                                                                    >  2:360171/1‑140 (MQ=255)                                                 aCCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAggg                                                                                    >  2:397415/1‑140 (MQ=255)                                                 aCCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAggg                                                                                    >  1:220957/1‑140 (MQ=255)                                                  ccTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCag                                                                                                 >  1:22238/1‑126 (MQ=255)                                                  ccTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCag                                                                                                 <  2:22238/126‑1 (MQ=255)                                                   cTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTACCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGc                                                                                  <  2:350712/140‑1 (MQ=255)                                                           aGGAGTTAGCCGCGACAATCGACCAACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCttt                                                                          >  2:319437/1‑140 (MQ=255)                                                                 tAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCg                                                                    <  1:360171/140‑1 (MQ=255)                                                                  aGCCGCGACAATCGAGCGACTGGAAGCACTGGATCGAAATGAACTGCGTAAACAGTGTTGCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAGGAGTTAGAGGGGCAACTCTTTGCCTGGa                                                                   <  2:55068/140‑1 (MQ=255)                                                                   gCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGAt                                                                  >  2:232572/1‑140 (MQ=255)                                                                            aTCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGa                                                         <  1:535159/140‑1 (MQ=255)                                                                                   gacTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGaa                                                  <  1:397415/140‑1 (MQ=255)                                                                                               tGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAgc                                      >  1:470318/1‑140 (MQ=255)                                                                                               tGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAgc                                      >  2:513825/1‑140 (MQ=255)                                                                                                  aTCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGcgcg                                   >  1:350474/1‑140 (MQ=255)                                                                                                  aTCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGcgcg                                   >  2:220119/1‑140 (MQ=255)                                                                                                           aaCTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGt                          >  2:73569/1‑140 (MQ=255)                                                                                                             cTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAgc                                      <  2:331756/126‑1 (MQ=255)                                                                                                             cTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAgc                                      >  1:331756/1‑126 (MQ=255)                                                                                                             cTGCGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTAt                        >  2:176096/1‑140 (MQ=255)                                                                                                                cGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAgc                                      <  2:297441/123‑1 (MQ=255)                                                                                                                cGTAAACAGTTTTCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAgc                                      >  1:297441/1‑123 (MQ=255)                                                                                                                         ttttCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAgg            <  1:176096/140‑1 (MQ=255)                                                                                                                          tttCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTAtgctgc                   >  2:431891/1‑132 (MQ=255)                                                                                                                          tttCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTAtgctgc                   <  1:431891/132‑1 (MQ=255)                                                                                                                          tttCCATTAAGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAggg           >  1:7145/1‑140 (MQ=255)                                                                                                                                   aGCGATTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATgcg  >  2:123549/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       CTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCGGGAGTGACCTTTTCAGAAGAGTTAGAGGGGCAACTCTTTGCCTCGATCATGCTTGATATGGAAAAGCTGATTAGCGCGTATCGACGTATGCTGCGACAAGGGAATCATGCG  >  NC_000913/2387147‑2387416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑