Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,192	Δ1 bp	coding (768/1431 nt)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,191	0	T	.	100.0%	156.7 / NA	38	coding (769/1431 nt)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (21/17);  total (21/17)

ATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAG  >  NC_000913/7052‑7318                                                                                                                                            |                                                                                                                                aTGCCAATCATCTGGACAATCCCTTGCGCTGCGGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCt                                                                                                                                 <  2:424849/140‑1 (MQ=255) aTGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCt                                                                                                                                 <  2:1217858/140‑1 (MQ=255) aTGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCt                                                                                                                                 <  2:1159981/140‑1 (MQ=255)    ccAATCATTTGGAAAACCCTTTGGGCTGCGGGATGGGGCGGCCAGGCCGCCGCGGCCGCTGCCGCTTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCtaag                                                                                                                              <  2:816827/140‑4 (MQ=255)         catcGGGACAATCCCTGGCCTCCCCGGAGGCGGAGGCCAGGACGCCGCTGCCGCTGCGGCGTTTGGCGTCGACCCCATCCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGtata                                                                                                                        <  2:46999/140‑1 (MQ=255)                  aaccccTGGCGCCGCCGGATGCGGGGCCGAGGACCCCGCTCCCGTCGCCGCGTTGGGCGCCAACCCCATCCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCcgccgc                                                                                                               <  2:1232041/137‑1 (MQ=255)                      ccTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCcgccgcgccg                                                                                                           <  1:1110824/140‑1 (MQ=255)                                                 gccgccgcTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCa                                                                                <  1:1146537/138‑1 (MQ=255)                                                 gccgccgcTGCCGCTGCCGCGTTTGGCGTCGACCCCATCCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCa                                                                                <  1:1215055/138‑1 (MQ=255)                                                 gACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCa                                                                                >  2:722252/1‑140 (MQ=255)                                                 gACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCa                                                                                <  1:201992/140‑1 (MQ=255)                                                      cgctgccgctgcCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTgc                                                                                            <  1:1029377/123‑1 (MQ=255)                                                      cgctgccgctgcCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTgc                                                                                            >  2:1029377/1‑123 (MQ=255)                                                      cgctgccgctgcCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACtctc                                                                           >  2:1109899/1‑140 (MQ=255)                                                      cgctgccgctgcCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACtctc                                                                           >  2:766413/1‑140 (MQ=255)                                                      cgctgccgctgcCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACtctc                                                                           >  2:264484/1‑140 (MQ=255)                                                               tgccgcTTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGa                                                                  <  1:1042636/140‑1 (MQ=255)                                                                                     aTTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGc                                            >  1:404819/1‑140 (MQ=255)                                                                                          cGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGat                                       <  1:766413/140‑1 (MQ=255)                                                                                              tCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTc                                   >  2:288783/1‑140 (MQ=255)                                                                                                aTTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCAt                                 >  1:56815/1‑140 (MQ=255)                                                                                                aTTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCAt                                 >  1:549909/1‑140 (MQ=255)                                                                                                      aaaCATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCa                           <  1:288783/140‑1 (MQ=255)                                                                                                      aaaCATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCa                           <  1:321945/140‑1 (MQ=255)                                                                                                      aaaCATACTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATACCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCa                           <  2:223458/140‑1 (MQ=255)                                                                                                            aCTGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTAc                     >  2:372286/1‑140 (MQ=255)                                                                                                              tGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCa                           <  1:1110643/132‑1 (MQ=255)                                                                                                              tGCGCTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCa                           >  2:1110643/1‑132 (MQ=255)                                                                                                                cgcTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCAATATTCATTACGCAAATTACCAgg                 >  2:1210867/1‑140 (MQ=255)                                                                                                                cgcTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATAGCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGAAGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAgg                 >  2:1112068/1‑140 (MQ=255)                                                                                                                 gcTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGc                >  2:971674/1‑140 (MQ=255)                                                                                                                 gcTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGc                >  2:1254127/1‑140 (MQ=255)                                                                                                                  cTGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCt               >  1:547501/1‑140 (MQ=255)                                                                                                                   tGAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTg              >  2:1180690/1‑140 (MQ=255)                                                                                                                    gAAAACCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTgg             <  1:264484/140‑1 (MQ=255)                                                                                                                     aaaaCCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGt            <  2:549909/140‑1 (MQ=255)                                                                                                                     aaaaCCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGt            <  1:1101652/140‑1 (MQ=255)                                                                                                                     aaaaCCGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGt            >  2:1101652/1‑140 (MQ=255)                                                                                                                          cGTTAGTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTa       >  2:134188/1‑140 (MQ=255)                                                                                                                               gTAATCGCCTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAg  >  1:512406/1‑140 (MQ=255)                                                                                                                                      ccTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGt            <  1:1000352/123‑1 (MQ=255)                                                                                                                                      ccTGGC‑TAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGt            >  2:1000352/1‑123 (MQ=255)                                                                                                                                            |                                                                                                                                ATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAG  >  NC_000913/7052‑7318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑