Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,931,183 (G)7→6 intergenic (‑90/‑133) ravA ← / → kup regulatory ATPase RavA/K(+):H(+) symporter Kup

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,931,1770G.100.0% 389.1 / NA 92intergenic (‑84/‑139)ravA/kupregulatory ATPase RavA/K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (48/44);  total (48/44)

CAACGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAGGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGACT  >  NC_000913/3931040‑3931310
                                                                                                                                         |                                                                                                                                     
cAACGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAggg                                                                                                                                     <  1:180454/140‑1 (MQ=255)
  aCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAggggg                                                                                                                                   <  2:23601/140‑1 (MQ=255)
    gAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAggggggt                                                                                                                                 >  2:944277/1‑139 (MQ=255)
       cTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAggggggtatt                                                                                                                              >  1:979880/1‑136 (MQ=255)
       cTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAggggggtatt                                                                                                                              >  1:344390/1‑136 (MQ=255)
       cTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAggggggtatt                                                                                                                              <  2:1169109/140‑5 (MQ=255)
          cTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTAttttt                                                                                                                          >  1:937730/1‑140 (MQ=255)
            cAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCt                                                                                                                        >  2:742577/1‑140 (MQ=255)
             aGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCtt                                                                                                                       <  2:344390/140‑1 (MQ=255)
             aGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCtt                                                                                                                       <  1:748071/140‑1 (MQ=255)
             aGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCtt                                                                                                                       <  2:706825/140‑1 (MQ=255)
                 gggAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAg                                                                                                                   <  1:927776/140‑1 (MQ=255)
                  ggAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGAATTTTTCtttagcc                                                                                                                  >  1:195247/1‑136 (MQ=255)
                    aaaTTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAg                                                                                                                   >  1:313329/1‑137 (MQ=255)
                    aaaTTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAg                                                                                                                   <  2:313329/137‑1 (MQ=255)
                         cTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCtt                                                                                                                       >  1:223064/1‑128 (MQ=255)
                         cTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCtt                                                                                                                       <  2:223064/128‑1 (MQ=255)
                            tCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAAtg                                                                                                        >  1:1217829/1‑140 (MQ=255)
                               gCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTgg                                                                                                     <  1:1048448/140‑1 (MQ=255)
                                 ttataaATGAAGGTGGGCCAAAAAGAAAGGGGGTCCTTTCGGCAAAAATTCTTCCTAAATTGCGAGTAAAGACCTTTTCTGCTGGTTGGTAAAAAAGGCTCCAA‑AGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGcc                                                                                                   <  2:195247/138‑1 (MQ=255)
                                          aGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTgg                                                                                                     >  1:1113041/1‑129 (MQ=255)
                                          aGGGTGAGCCATAATGAAGTGGCGTCCTTCCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTgg                                                                                                     <  2:1113041/129‑1 (MQ=255)
                                            ggTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAg                                                                                        >  1:334125/1‑140 (MQ=255)
                                             gTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTTAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGt                                                                                       >  1:675048/1‑140 (MQ=255)
                                             gTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGt                                                                                       >  1:996998/1‑140 (MQ=255)
                                             gTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGt                                                                                       >  2:394122/1‑140 (MQ=255)
                                             gTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGCATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGt                                                                                       >  1:459104/1‑140 (MQ=255)
                                                aGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGcc                                                                                                   <  2:749992/125‑1 (MQ=255)
                                                aGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGcc                                                                                                   >  1:749992/1‑125 (MQ=255)
                                                aGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACg                                                                                    >  2:655495/1‑140 (MQ=255)
                                                aGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACg                                                                                    >  2:84892/1‑140 (MQ=255)
                                                aGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGGACg                                                                                    >  2:819073/1‑140 (MQ=255)
                                                aGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAA‑‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGa                                                                                   >  2:47531/1‑140 (MQ=255)
                                                 gCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGa                                                                                   >  2:1067717/1‑140 (MQ=255)
                                                 gCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGa                                                                                   >  1:593769/1‑140 (MQ=255)
                                                   cATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGAtt                                                                                 <  1:1067717/140‑1 (MQ=255)
                                                    aTAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGAttt                                                                                <  1:415240/140‑1 (MQ=255)
                                                          aaGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGt                                                                          <  1:655495/140‑1 (MQ=255)
                                                          aaGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGt                                                                          <  2:937730/140‑1 (MQ=255)
                                                           aGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGtt                                                                         >  2:764167/1‑140 (MQ=255)
                                                           aGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGtt                                                                         >  1:242796/1‑140 (MQ=255)
                                                           aGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGtt                                                                         >  2:554009/1‑140 (MQ=255)
                                                            gTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTa                                                                        >  1:405102/1‑140 (MQ=255)
                                                            gTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTa                                                                        >  1:860443/1‑140 (MQ=255)
                                                            gTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTa                                                                        >  1:1041576/1‑140 (MQ=255)
                                                             tGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTaa                                                                       >  1:396249/1‑140 (MQ=255)
                                                             tGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTaa                                                                       >  1:378073/1‑140 (MQ=255)
                                                             tGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTaa                                                                       >  2:1255549/1‑140 (MQ=255)
                                                             tGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTaa                                                                       <  2:979880/140‑1 (MQ=255)
                                                               gCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATc                                                                     >  2:453430/1‑140 (MQ=255)
                                                                  tCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTg                                                                  >  2:908209/1‑140 (MQ=255)
                                                                    cTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCa                                                                >  2:629526/1‑140 (MQ=255)
                                                                       tCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATAc                                                             >  2:643056/1‑140 (MQ=255)
                                                                       tCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATAc                                                             >  1:1046109/1‑140 (MQ=255)
                                                                         gTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACtg                                                           >  2:865578/1‑140 (MQ=255)
                                                                          tCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACtgt                                                          <  1:394122/140‑1 (MQ=255)
                                                                          tCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACtgt                                                          <  1:739323/140‑1 (MQ=255)
                                                                          tCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACtgt                                                          <  2:378073/140‑1 (MQ=255)
                                                                            aaaaGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTgc                                                        >  1:1253063/1‑140 (MQ=255)
                                                                               aGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCt                                                     <  2:1253063/140‑1 (MQ=255)
                                                                                     gCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTtg                                               >  2:1133403/1‑140 (MQ=255)
                                                                                     gCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTtg                                               <  1:554009/140‑1 (MQ=255)
                                                                                     gCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTtg                                               <  1:764167/140‑1 (MQ=255)
                                                                                      cGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTtgt                                              >  1:1090710/1‑140 (MQ=255)
                                                                                      cGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAA‑‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTtgtg                                             <  1:47531/140‑1 (MQ=255)
                                                                                       gTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTtgtg                                             <  1:316403/140‑1 (MQ=255)
                                                                                       gTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTtgtg                                             <  1:629526/140‑1 (MQ=255)
                                                                                           aTTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGcc                                         <  2:860737/140‑1 (MQ=255)
                                                                                            ttGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCa                                        <  1:84892/140‑1 (MQ=255)
                                                                                                gAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTTAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAggg                                    <  2:675048/140‑1 (MQ=255)
                                                                                                gAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAggg                                    <  1:944277/140‑1 (MQ=255)
                                                                                                gAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAggg                                    >  2:367221/1‑140 (MQ=255)
                                                                                                  gTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGAc                                  <  2:242796/140‑1 (MQ=255)
                                                                                                          gTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGcaca                          <  2:271821/140‑1 (MQ=255)
                                                                                                          gTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGcaca                          <  2:1041576/140‑1 (MQ=255)
                                                                                                            ttCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGcacaca                        <  2:860443/140‑1 (MQ=255)
                                                                                                            ttCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGcacaca                        <  1:1255549/140‑1 (MQ=255)
                                                                                                             tCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACAt                       >  1:832579/1‑140 (MQ=255)
                                                                                                             tCTTGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACAt                       >  2:311409/1‑140 (MQ=255)
                                                                                                               ttGCTGGTGGCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACAttt                     >  1:448124/1‑140 (MQ=255)
                                                                                                                      tggCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATAttt              >  1:922107/1‑140 (MQ=255)
                                                                                                                      tggCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATAttt              <  1:742577/140‑1 (MQ=255)
                                                                                                                        gCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCa            >  2:456941/1‑140 (MQ=255)
                                                                                                                        gCTAAAATAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCa            >  2:55131/1‑140 (MQ=255)
                                                                                                                           aaaaTAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACg         <  1:55131/140‑1 (MQ=255)
                                                                                                                           aaaaTAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACg         <  1:367221/140‑1 (MQ=255)
                                                                                                                              aTAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGaaa      <  1:1133403/140‑1 (MQ=255)
                                                                                                                               tAGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAg     <  2:223200/140‑1 (MQ=255)
                                                                                                                                aGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGa    <  2:69619/140‑1 (MQ=255)
                                                                                                                                aGTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGa    <  1:453430/140‑1 (MQ=255)
                                                                                                                                 gTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGAc   <  2:459104/140‑1 (MQ=255)
                                                                                                                                 gTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGAc   <  1:456941/140‑1 (MQ=255)
                                                                                                                                 gTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGAc   <  1:467171/140‑1 (MQ=255)
                                                                                                                                 gTCTCAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGAc   <  2:405102/140‑1 (MQ=255)
                                                                                                                                  tctcAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGTCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGACt  >  2:274375/1‑140 (MQ=255)
                                                                                                                                  tctcAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGACt  >  2:808695/1‑140 (MQ=255)
                                                                                                                                  tctcAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGACt  >  1:765567/1‑140 (MQ=255)
                                                                                                                                    tcAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGAc   >  2:1251673/1‑137 (MQ=255)
                                                                                                                                    tcAAA‑GGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGAc   <  1:1251673/137‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
CAACGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAGGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGACT  >  NC_000913/3931040‑3931310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑