BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,211,898	Δ1 bp	coding (106/324 nt)	iraM ←	anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,211,896	0	A	.	100.0%	454.5 / NA	107	coding (108/324 nt)	iraM	anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (52/55); total (52/55)

Only 100 of 111 total aligned reads displayed.

GAGGACACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATT  >  NC_000913/1211757‑1212028

gaggaCACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGgaga                                                                                                                                      <  1:597659/140‑1 (MQ=255)

gaggaCACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGgaga                                                                                                                                      <  1:241971/140‑1 (MQ=255)

   gaCACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGgaga                                                                                                                                      >  2:92763/1‑137 (MQ=255)

   gaCACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGgaga                                                                                                                                      <  1:92763/137‑1 (MQ=255)

      acTCTTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATAtag                                                                                                                               <  1:722304/140‑1 (MQ=255)

       ctctTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATAtagt                                                                                                                              >  1:563761/1‑140 (MQ=255)

          ttGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGa                                                                                                                           <  2:1185185/140‑1 (MQ=255)

              cTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGa                                                                                                                       <  2:819191/140‑1 (MQ=255)

                        gTAAACTCCATAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAgatga                                                                                                             <  2:959611/140‑1 (MQ=255)

                           aaCTCCATAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCa                                                                                                          >  1:200903/1‑140 (MQ=255)

                              tCCATAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCAttt                                                                                                       <  1:435881/140‑1 (MQ=255)

                                cATAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGGTAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTc                                                                                                     >  1:520290/1‑140 (MQ=255)

                                 aTAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCa                                                                                                    <  2:705666/140‑1 (MQ=255)

                                 aTAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCa                                                                                                    >  1:446566/1‑140 (MQ=255)

                                 aTAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCa                                                                                                    <  1:639554/140‑1 (MQ=255)

                                   aaaTCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGGTAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCata                                                                                                  <  2:520290/140‑1 (MQ=255)

                                   aaaTCCTTGTTGAATGGTGCGATGTGAAAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCata                                                                                                  <  1:920458/140‑1 (MQ=255)

                                        cTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTAcc                                                                                             >  1:1227916/1‑140 (MQ=255)

                                          tgttgAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTAcccc                                                                                           >  1:63609/1‑140 (MQ=255)

                                            ttgAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCat                                                                                         <  2:937909/140‑1 (MQ=255)

                                                 tGGTGCGATGTGATGAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGc                                                                                    >  1:1060483/1‑140 (MQ=255)

                                                   gTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTg                                                                                  >  2:636415/1‑140 (MQ=255)

                                                    tGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATAGCTGa                                                                                 >  2:326388/1‑140 (MQ=255)

                                                     gCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGaa                                                                                <  1:88033/140‑1 (MQ=255)

                                                           tgATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATAGCTGAAAAAGat                                                                          <  1:326388/140‑1 (MQ=255)

                                                              tAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGAtata                                                                       >  2:1214769/1‑140 (MQ=255)

                                                               aaaTAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATAc                                                                      <  2:148904/140‑1 (MQ=255)

                                                               aaaTAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATAc                                                                      <  1:636415/140‑1 (MQ=255)

                                                               aaaTAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATAc                                                                      <  2:446566/140‑1 (MQ=255)

                                                                    gTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACAt                                                                 <  1:110334/140‑1 (MQ=255)

                                                                    gTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACAt                                                                 <  2:910037/140‑1 (MQ=255)

                                                                     tAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATg                                                                >  2:74530/1‑140 (MQ=255)

                                                                     tAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATg                                                                >  2:1043926/1‑140 (MQ=255)

                                                                     tAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATg                                                                <  1:1043926/140‑1 (MQ=255)

                                                                     tAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATg                                                                >  1:1075524/1‑140 (MQ=255)

                                                                     tAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATg                                                                <  1:1089764/140‑1 (MQ=255)

                                                                       aTAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATa                                                                                                                     >  1:147868/1‑85 (MQ=255)

                                                                       aTAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATa                                                                                                                     <  2:147868/85‑1 (MQ=255)

                                                                       aTAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATAc                                                                      <  2:1230679/132‑1 (MQ=255)

                                                                       aTAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATAc                                                                      >  1:1230679/1‑132 (MQ=255)

                                                                                   ttATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTAcc                                                  >  2:1221176/1‑140 (MQ=255)

                                                                                    tATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCg                                                 >  2:786416/1‑140 (MQ=255)

                                                                                      tCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCgtg                                               <  2:1060483/140‑1 (MQ=255)

                                                                                       ccTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCgtgt                                              <  2:510700/140‑1 (MQ=255)

                                                                                         ttAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTAcc                                                  <  1:1229040/134‑1 (MQ=255)

                                                                                          tAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCaa                                           >  2:127587/1‑140 (MQ=255)

                                                                                          tAAGGATAATACCAGACTTAACCGGTGCAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCaa                                           >  1:613872/1‑140 (MQ=255)

                                                                                           aaGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAAt                                          >  1:1139733/1‑140 (MQ=255)

                                                                                            aGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAAtt                                         >  2:1175421/1‑140 (MQ=255)

                                                                                                 aataCCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTAt                                    >  1:107590/1‑140 (MQ=255)

                                                                                                   taCCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATcc                                  >  2:694265/1‑140 (MQ=255)

                                                                                                   taCCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATcc                                  >  1:263282/1‑140 (MQ=255)

                                                                                                     ccAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCAc                                >  1:1233151/1‑140 (MQ=255)

                                                                                                     ccAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCAc                                <  2:964959/140‑1 (MQ=255)

                                                                                                        gACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTc                                             >  1:221924/1‑124 (MQ=255)

                                                                                                        gACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTc                                             <  2:221924/124‑1 (MQ=255)

                                                                                                        gACTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTc                             >  2:71570/1‑140 (MQ=255)

                                                                                                         aCTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCa                            <  1:945110/140‑1 (MQ=255)

                                                                                                         aCTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCa                            >  2:945110/1‑140 (MQ=255)

                                                                                                          cTTAACCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCAt                           <  2:411799/140‑1 (MQ=255)

                                                                                                             aaCCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTg                        <  1:1214769/140‑1 (MQ=255)

                                                                                                              aCCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATc                                   <  2:59126/128‑1 (MQ=255)

                                                                                                              aCCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATc                                   >  1:59126/1‑128 (MQ=255)

                                                                                                              aCCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGt                       >  2:102191/1‑140 (MQ=255)

                                                                                                              aCCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGt                       >  1:841826/1‑140 (MQ=255)

                                                                                                              aCCGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGt                       >  1:150102/1‑140 (MQ=255)

                                                                                                               ccGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGtt                      <  2:841826/140‑1 (MQ=255)

                                                                                                               ccGGTGTAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGtt                      <  2:149695/140‑1 (MQ=255)

                                                                                                                   tgtAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTGTCCACTTCATTTGTTATGt                  >  2:550851/1‑140 (MQ=255)

                                                                                                                   tgtAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGt                  >  1:534056/1‑140 (MQ=255)

                                                                                                                   tgtAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGt                  >  1:846211/1‑140 (MQ=255)

                                                                                                                   tgtAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGt                  >  1:1068355/1‑140 (MQ=255)

                                                                                                                     tAAATATACTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGGCAATTACTATCCACTTCATTTGTTATGTCt                >  2:769963/1‑140 (MQ=255)

                                                                                                                            aCTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa         <  2:846211/140‑1 (MQ=255)

                                                                                                                            aCTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa         >  1:1076189/1‑140 (MQ=255)

                                                                                                                            aCTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa         <  1:102191/140‑1 (MQ=255)

                                                                                                                            aCTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa         <  2:600394/140‑1 (MQ=255)

                                                                                                                            aCTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa         <  2:534056/140‑1 (MQ=255)

                                                                                                                            aCTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa         <  1:1148572/140‑1 (MQ=255)

                                                                                                                            aCTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa         <  1:1175421/140‑1 (MQ=255)

                                                                                                                            aCTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa         <  2:1076189/140‑1 (MQ=255)

                                                                                                                            aCTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa         <  2:150102/140‑1 (MQ=255)

                                                                                                                            aCTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa         <  1:769963/140‑1 (MQ=255)

                                                                                                                            aCTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa         <  1:74530/140‑1 (MQ=255)

                                                                                                                             cTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTGTCCACTTCATTTGTTATGTCTTATCCCAc        <  1:550851/140‑1 (MQ=255)

                                                                                                                             cTGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCAc        <  1:359342/140‑1 (MQ=255)

                                                                                                                              tGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACg       <  1:88731/140‑1 (MQ=255)

                                                                                                                              tGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACg       <  1:537090/140‑1 (MQ=255)

                                                                                                                              tGCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACg       <  1:71570/140‑1 (MQ=255)

                                                                                                                               gCCAGGAGGGAG‑ATTATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATAc                                                                      <  2:1123059/76‑1 (MQ=255)

                                                                                                                               gCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATAc                                                                      >  1:1123059/1‑76 (MQ=255)

                                                                                                                               gCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa         >  1:679480/1‑137 (MQ=255)

                                                                                                                               gCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa         <  2:679480/137‑1 (MQ=255)

                                                                                                                               gCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACgg      >  1:169576/1‑140 (MQ=255)

                                                                                                                               gCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACg       >  1:608953/1‑139 (MQ=255)

                                                                                                                               gCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACg       <  2:280207/139‑1 (MQ=255)

                                                                                                                               gCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACg       >  1:280207/1‑139 (MQ=255)

                                                                                                                               gCCAGGAGGGAG‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACg       <  2:608953/139‑1 (MQ=255)

                                                                                                                                   ggagggag‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTAtt  <  1:694265/140‑1 (MQ=255)

                                                                                                                                   ggagggag‑AATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTAtt  <  2:730060/140‑1 (MQ=255)

GAGGACACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAATGGTGCGATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATT  >  NC_000913/1211757‑1212028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`