Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,984	(G)7→8	coding (1041/1245 nt)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,977	1	.	G	100.0%	61.7 / NA	19	A345G (GCG→GGG)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (2/17);  total (2/17)

AATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGA  >  NC_000913/3240838‑3241105                                                                                                                                             |                                                                                                                                 aaTATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTCCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGc                                                                                                                                   <  1:238188/140‑1 (MQ=255)  atatGGCGGGCGCAGCAATCTCTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑g                                                                                                                                 <  1:472005/140‑1 (MQ=255)          ggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggt                                                                                                                         <  1:959724/140‑3 (MQ=255)             gcagcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggtctc                                                                                                                      <  1:1137374/140‑6 (MQ=255)                    tCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctgctgct                                                                                                                >  1:87340/1‑140 (MQ=255)                    tCACTATTACAGTGCTGACGCTGGCTGCGGATAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctgctgct                                                                                                                <  2:773572/140‑1 (MQ=255)                           tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCa                                                                                                         <  2:266642/140‑1 (MQ=255)                           tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCa                                                                                                         <  2:821891/140‑1 (MQ=255)                            aCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCAc                                                                                                        >  2:557709/1‑140 (MQ=255)                               gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                                                     >  2:574350/1‑140 (MQ=255)                                tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                    <  1:35614/140‑1 (MQ=255)                                tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACTGGc                                                                                                    >  1:785542/1‑140 (MQ=255)                                  ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCt                                                                                                  >  1:1120601/1‑140 (MQ=255)                                  ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCt                                                                                                  >  1:281320/1‑140 (MQ=255)                                    gACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACCGGCCTGt                                                                                                >  2:248956/1‑140 (MQ=255)                                     aCGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                                                               <  1:557709/140‑1 (MQ=255)                                       gctggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAat                                                                                             <  1:869129/140‑1 (MQ=255)                                            ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGtt                                                                                        <  2:778431/140‑1 (MQ=255)                                                     aTACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCCCTGGCCCGTAATATGTTTGGGAtttt                                                                               >  2:884114/1‑139 (MQ=255)                                                      tACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCg                                                                              <  2:362092/140‑1 (MQ=255)                                                        cGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                                            <  1:574350/140‑1 (MQ=255)                                                        cGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                                            <  1:325177/140‑1 (MQ=255)                                                        cGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                                            <  1:884114/140‑1 (MQ=255)                                                           tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                         <  2:1120601/140‑1 (MQ=255)                                                                aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGGTGATTCCCCTGGCCTGTAATATGTTCGGGATTTCGAACGATATCg                                                                    >  2:1003030/1‑140 (MQ=255)                                                                                   cGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGt                                                 <  1:111106/140‑1 (MQ=255)                                                                                     gcgcTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCg                                               <  2:281320/140‑1 (MQ=255)                                                                                              ttGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatca                                      >  1:957690/1‑140 (MQ=255)                                                                                                 aGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCg                                   <  1:163476/140‑1 (MQ=255)                                                                                                   cGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTTCTGCTTATCCCACTGGCCCGTAATATTTTTGGGATTTCGAAAGATATCGCCATGCAGGGGGTTGCCCTCGGCTTTTTCATCGGc                                 >  2:142904/1‑140 (MQ=255)                                                                                                    gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                                <  2:110395/140‑1 (MQ=255)                                                                                                     tggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACCGGCCTGTAATATGTTCGGGATTTCGAACGATATCGCCATGCAGGGGGGTGCCGGCGGGTTTTTCATCGGCgg                               >  2:163476/1‑139 (MQ=255)                                                                                                        tggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCCCCGGCCCGTAAAATGTTTGGGATTTCGAACGATATCGCCCTGCAGGGGGGTGCCGTCGGCTTTTTCATCCGCGGAtt                            >  2:874802/1‑140 (MQ=255)                                                                                                                    gtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCCCTGGCCTGTAATATGTTCGGGATTTTGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTg                >  2:494891/1‑140 (MQ=255)                                                                                                                     tgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCCCCGGCCCGGAATATGTTCGGGATTTTCAACGATATCGCCCTGCCGGGGGGTGGCGGCCGGTTTTTTATCGGCGGATTTCAGGGCTCtttc               >  2:796131/1‑138 (MQ=255)                                                                                                                     tgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGGGGGTGCCGTCGGCTTTATCATCGGCGGATTGCAGGACTCtttc               >  2:328104/1‑138 (MQ=255)                                                                                                                       ccTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACCGGCCTGTAATATGTTCGGGATTTTGAACGATATCGCCATGCAGGGGGGTGCCGTTGGCTTTTTCATCGGCGGATTTCAGGACTCTTGCGa             >  2:863505/1‑140 (MQ=255)                                                                                                                         tgtgGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGaaa           <  1:425831/140‑1 (MQ=255)                                                                                                                                  tCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACCGGCCTGTAATATGTTCGGGATTTCGGACGATATCGCCATTCAGGGGGTTGGCCTCGGCTTTATCATCGGCGGATTTCAGGACTCTTGCGAAAACgcgccgg  >  2:914990/1‑137 (MQ=255)                                                                                                                                             |                                                                                                                                 AATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGA  >  NC_000913/3240838‑3241105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑