Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,594,256	(C)8→9	pseudogene (3831/5422 nt)	yneO ←	AIDA‑I family autotransporter YneO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,594,248	1	.	C	100.0%	210.2 / NA	61	pseudogene (3839/5422 nt)	yneO	AIDA‑I family autotransporter YneO
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (35/26);  total (35/26)

AGCGACACGCAGTGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTG  >  NC_000913/1594111‑1594379                                                                                                                                           |                                                                                                                                     aGCGACACGCAGTGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑‑cc                                                                                                                                   >  2:1080302/1‑140 (MQ=255)                cccccccAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATc                                                                                                                     >  2:1226368/1‑140 (MQ=255)                   ccccAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCcgt                                                                                                                  >  1:440362/1‑140 (MQ=255)                    cccAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCcgtc                                                                                                                 >  2:1133576/1‑140 (MQ=255)                    cccAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCcgtc                                                                                                                 >  2:1177665/1‑140 (MQ=255)                     ccAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCcgtcg                                                                                                                <  1:1080302/140‑1 (MQ=255)                       aGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTa                                                                                                              >  1:614289/1‑140 (MQ=255)                            tAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTg                                                                                                         >  2:686796/1‑140 (MQ=255)                                       tCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCgg                                                                                              <  2:1168679/140‑1 (MQ=255)                                            tGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCg                                                                                         >  1:20910/1‑140 (MQ=255)                                                 tggtgACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCgttgtt                                                                                    <  1:573679/140‑1 (MQ=255)                                                 tggtgACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCgttgtt                                                                                    >  1:571034/1‑140 (MQ=255)                                                   gtgACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTaa                                                                                  <  2:20910/140‑1 (MQ=255)                                                   gtgACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTaa                                                                                  <  2:571034/140‑1 (MQ=255)                                                   gtgACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTaa                                                                                  >  1:612669/1‑140 (MQ=255)                                                                 cATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCg                                                                    >  2:684603/1‑140 (MQ=255)                                                                  aTCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCCCGGAATCGTTGTTAAGAGTCAGGATCCCgg                                                                   >  1:194814/1‑140 (MQ=255)                                                                            tGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGttt                                                         >  1:902230/1‑140 (MQ=255)                                                                              ttATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTg                                                       >  1:777150/1‑140 (MQ=255)                                                                                aTGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTc                                                     >  2:73729/1‑140 (MQ=255)                                                                                aTGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTc                                                     >  1:563433/1‑140 (MQ=255)                                                                                   gATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTcaca                                                  >  2:789295/1‑140 (MQ=255)                                                                                        tAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCacc                                             >  2:790165/1‑140 (MQ=255)                                                                                        tAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCacc                                             >  2:531617/1‑140 (MQ=255)                                                                                        tAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCacc                                             >  1:1116370/1‑140 (MQ=255)                                                                                         aaTGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccg                                            <  2:440362/140‑1 (MQ=255)                                                                                           tgtgTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgct                                          <  1:541245/140‑1 (MQ=255)                                                                                             tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctac                                        <  1:531617/140‑1 (MQ=255)                                                                                             tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctac                                        <  1:1005954/140‑1 (MQ=255)                                                                                              gtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctacc                                       >  2:1043795/1‑140 (MQ=255)                                                                                               ttGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctacc                                       >  2:655237/1‑140 (MQ=255)                                                                                               ttGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctaccg                                      >  2:983375/1‑140 (MQ=255)                                                                                               ttGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctaccg                                      >  2:936537/1‑140 (MQ=255)                                                                                               ttGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctaccg                                      <  2:194814/140‑1 (MQ=255)                                                                                               ttGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctaccg                                      >  2:778229/1‑140 (MQ=255)                                                                                                     tGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgct                                          >  1:167127/1‑130 (MQ=255)                                                                                                     tGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgct                                          <  2:167127/130‑1 (MQ=255)                                                                                                     tGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATa                                <  1:1133576/140‑1 (MQ=255)                                                                                                     tGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATa                                <  1:1177665/140‑1 (MQ=255)                                                                                                       ccATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCa                                               >  2:759830/1‑123 (MQ=255)                                                                                                       ccATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCa                                               <  1:759830/123‑1 (MQ=255)                                                                                                                 ggCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATc                    >  2:442859/1‑140 (MQ=255)                                                                                                                 ggCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATc                    >  1:299852/1‑140 (MQ=255)                                                                                                                  gCAGAGTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCg                   <  1:73729/140‑1 (MQ=255)                                                                                                                    agagTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGcc                 >  1:117167/1‑140 (MQ=255)                                                                                                                      agTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGcc                 <  2:855792/138‑1 (MQ=255)                                                                                                                      agTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGcc                 >  1:855792/1‑138 (MQ=255)                                                                                                                      agTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCa               >  2:292948/1‑140 (MQ=255)                                                                                                                      agTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCa               >  1:794767/1‑140 (MQ=255)                                                                                                                      agTCGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCa               >  2:1160267/1‑140 (MQ=255)                                                                                                                         cGGAACCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCt            >  1:36957/1‑140 (MQ=255)                                                                                                                            aaCCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACg         <  2:563433/140‑1 (MQ=255)                                                                                                                            aaCCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACg         <  2:614289/140‑1 (MQ=255)                                                                                                                            aaCCGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACg         <  1:469332/140‑1 (MQ=255)                                                                                                                               cGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTc      <  1:686796/140‑1 (MQ=255)                                                                                                                               cGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTc      <  1:442859/140‑1 (MQ=255)                                                                                                                               cGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTc      <  1:778229/140‑1 (MQ=255)                                                                                                                               cGAAAGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTc      <  1:936537/140‑1 (MQ=255)                                                                                                                                 aaaGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAg    <  2:299852/140‑1 (MQ=255)                                                                                                                                 aaaGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAg    >  1:907519/1‑140 (MQ=255)                                                                                                                                  aaGCAATTTCCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAg    <  1:655237/140‑1 (MQ=255)                                                                                                                                   aGCAATTTC‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTg  <  2:777150/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                     AGCGACACGCAGTGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTG  >  NC_000913/1594111‑1594379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑