Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	241,471	(C)5→6	coding (1833/2445 nt)	fadE ←	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	241,466	1	.	C	100.0%	252.7 / NA	71	D613G (GAT→GGT)	fadE	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (38/33);  total (38/33)

CCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATAT‑CCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCG  >  NC_000913/241335‑241597                                                                                                                                     |                                                                                                                                    ccatcGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATAT‑ccccccag                                                                                                                             <  2:769873/140‑4 (MQ=255)   atcGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTGAAATAT‑ccccccagac                                                                                                                           >  2:697494/1‑135 (MQ=255)   atcGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATAT‑ccccccagac                                                                                                                           <  2:80297/140‑6 (MQ=255)   atcGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATAT‑ccccccagac                                                                                                                           <  2:503851/140‑6 (MQ=255)                ccTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATgcg                                                                                                               <  1:367090/140‑1 (MQ=255)                ccTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATgcg                                                                                                               >  1:1217669/1‑140 (MQ=255)                  tGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCt                                                                                                             <  1:1099284/140‑1 (MQ=255)                  tGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCt                                                                                                             <  1:1257179/140‑1 (MQ=255)                          cgcATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGGCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGt                                                                                                     >  2:880758/1‑140 (MQ=255)                            cATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGttt                                                                                                   >  1:598083/1‑140 (MQ=255)                              tCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCa                                                                                                 <  1:978162/140‑1 (MQ=255)                                 tGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGc                                                                                              <  1:524158/140‑1 (MQ=255)                                 tGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGc                                                                                              <  2:269042/140‑1 (MQ=255)                                  gTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCt                                                                                             >  2:1020690/1‑140 (MQ=255)                                      gCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGGGCTCGCGACGTTTCAGGCTgccg                                                                                         >  2:387548/1‑140 (MQ=255)                                       ccccAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTgccgc                                                                                        >  1:183072/1‑140 (MQ=255)                                        cccAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTgccgcc                                                                                       >  1:86774/1‑140 (MQ=255)                                        cccAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTgccgcc                                                                                       >  2:241860/1‑140 (MQ=255)                                           aGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAg                                                                                    >  2:171098/1‑140 (MQ=255)                                           aGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAg                                                                                    >  2:1130771/1‑140 (MQ=255)                                           aGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAg                                                                                    >  1:400609/1‑140 (MQ=255)                                           aGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAg                                                                                    >  2:375739/1‑140 (MQ=255)                                           aGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAg                                                                                    >  1:605839/1‑140 (MQ=255)                                             tGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCa                                                                                  >  2:1077437/1‑140 (MQ=255)                                             tGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCa                                                                                  >  1:1168747/1‑140 (MQ=255)                                             ggCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCa                                                                                  <  2:740483/139‑1 (MQ=255)                                                  cAGCGGCAGGTCGGCTTCATTACGACCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGcc                                                                             >  2:374634/1‑140 (MQ=255)                                                    gCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCAt                                                                           >  2:404552/1‑140 (MQ=255)                                                    gCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCAt                                                                           >  2:999052/1‑140 (MQ=255)                                                      ggCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCATCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCg                                                                         <  2:1168747/140‑1 (MQ=255)                                                       gCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCga                                                                        >  2:120459/1‑140 (MQ=255)                                                       gCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCga                                                                        >  1:363298/1‑140 (MQ=255)                                                         aGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCgaga                                                                      <  1:999052/140‑1 (MQ=255)                                                         aGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCgaga                                                                      <  1:241860/140‑1 (MQ=255)                                                             cGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATc                                                                  <  2:106965/140‑1 (MQ=255)                                                                cTTCATTACGGCCTTCGTCGTCATAACGGCTCAGCACGGCAGAGGCGAGGGAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTCCCATCGAGACATCAGa                                                               >  2:1127438/1‑140 (MQ=255)                                                                cTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGa                                                               >  2:823335/1‑140 (MQ=255)                                                                        cGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGGGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAggg                                                       <  1:387548/140‑1 (MQ=255)                                                                          gCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCg                                                     >  1:1150118/1‑140 (MQ=255)                                                                             ttCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAgg                                                  >  1:300257/1‑140 (MQ=255)                                                                              tcgtcgTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGt                                                 >  2:428265/1‑140 (MQ=255)                                                                              tcgtcgTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGt                                                 >  2:432259/1‑140 (MQ=255)                                                                              tcgtcgTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGt                                                 <  2:300257/140‑1 (MQ=255)                                                                               cgtcgtCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGtt                                                >  1:148675/1‑140 (MQ=255)                                                                                   gtcATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGgcg                                            >  2:791542/1‑140 (MQ=255)                                                                                     cATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCt                                          <  2:177936/140‑1 (MQ=255)                                                                                         aCGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAgg                                      <  2:715339/140‑1 (MQ=255)                                                                                         aCGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAgg                                      <  2:363298/140‑1 (MQ=255)                                                                                         aCGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAgg                                      <  2:1150118/140‑1 (MQ=255)                                                                                          cGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGc                                     >  2:1251077/1‑140 (MQ=255)                                                                                               cAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGc                                     <  2:436807/135‑1 (MQ=255)                                                                                               cAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGc                                     >  1:436807/1‑135 (MQ=255)                                                                                               cAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGtt                                 >  2:145878/1‑139 (MQ=255)                                                                                               cAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGtt                                 <  1:145878/139‑1 (MQ=255)                                                                                               cAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTc                                <  1:375739/140‑1 (MQ=255)                                                                                               cAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTc                                >  2:523897/1‑140 (MQ=255)                                                                                                aGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCa                               >  2:671686/1‑140 (MQ=255)                                                                                                aGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCa                               <  1:1077437/140‑1 (MQ=255)                                                                                                 gCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAg                              <  1:171098/140‑1 (MQ=255)                                                                                                 gCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAg                              <  1:340230/140‑1 (MQ=255)                                                                                                 gCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAg                              <  2:183072/140‑1 (MQ=255)                                                                                                 gCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAg                              <  1:1198625/140‑1 (MQ=255)                                                                                                  cACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGtt                                 >  1:813258/1‑136 (MQ=255)                                                                                                  cACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGtt                                 <  2:813258/136‑1 (MQ=255)                                                                                                   aCGGCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAGGt                            <  2:1217669/140‑1 (MQ=255)                                                                                                     ggCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAgg                                      >  1:16800/1‑128 (MQ=255)                                                                                                     ggCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAgg                                      <  2:16800/128‑1 (MQ=255)                                                                                                      gCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAGGTGCt                         <  1:671686/140‑1 (MQ=255)                                                                                                      gCAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGACGCTCAGGCGGTTCAGGTGCt                         <  2:86774/140‑1 (MQ=255)                                                                                                       cAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAGGt                            <  2:393401/136‑1 (MQ=255)                                                                                                       cAGAGGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAGGt                            >  1:393401/1‑136 (MQ=255)                                                                                                          agGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAGGTGCTGAta                     <  1:823335/140‑1 (MQ=255)                                                                                                          agGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAGGTGCTGAta                     >  2:1226900/1‑140 (MQ=255)                                                                                                          agGCGAGGTAGAGCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAGGTGCTGAta                     >  1:205168/1‑140 (MQ=255)                                                                                                                      gCTGGCTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAGGTGCTGATAGTAGCGTTTAGt         >  2:1251054/1‑140 (MQ=255)                                                                                                                           cTTAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCAt    <  1:1251077/140‑1 (MQ=255)                                                                                                                             tAAAATATCCCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCg  <  2:400609/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    CCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCGAGGTAGAGCTGGCTTAAAATAT‑CCCCCAGACGGGCCGAGATGCGCTCGCGACGTTTCAGGCTGCCGCCCAGCACTGCCATCGAGACATCAGAAAGCAGGGCGAGGTTGGCGCTCAGGCGGTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCG  >  NC_000913/241335‑241597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑