Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,101,039	(C)7→8	coding (585/720 nt)	yggN ←	putative EcfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,101,032	1	.	C	94.8%	187.9 / 3.2	58	coding (592/720 nt)	yggN	putative EcfF
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base C (22/33);  total (24/34)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01

AAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑CCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCGCG  >  NC_000913/3100894‑3101167                                                                                                                                            |                                                                                                                                         aaaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑c                                                                                                                                         <  2:804028/140‑1 (MQ=255) aaaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑c                                                                                                                                         <  1:1080443/140‑1 (MQ=255) aaaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑c                                                                                                                                         <  2:123873/140‑1 (MQ=255)  aaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑cc                                                                                                                                        <  2:1236009/140‑1 (MQ=255)     agagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑ccccc                                                                                                                                     <  1:468515/140‑1 (MQ=255)      gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGCAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑cccccc                                                                                                                                    >  2:1011851/1‑140 (MQ=255)      gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑cccccc                                                                                                                                    >  2:913969/1‑140 (MQ=255)      gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑cccccc                                                                                                                                    >  2:590254/1‑140 (MQ=255)      gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑cccccc                                                                                                                                    <  1:573871/140‑1 (MQ=255)      gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑cccccc                                                                                                                                    >  2:209734/1‑140 (MQ=255)           ttattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑ccccccccagg                                                                                                                               <  1:677060/140‑5 (MQ=255)           ttattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑ccccccccagg                                                                                                                               <  1:23628/140‑5 (MQ=255)            tattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑ccccccccaggc                                                                                                                              >  1:793562/1‑135 (MQ=255)                  aaaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCa                                                                                                                         <  1:24635/140‑1 (MQ=255)                     ttCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGca                                                                                                                      <  2:377738/140‑1 (MQ=255)                     ttCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGca                                                                                                                      >  1:377738/1‑140 (MQ=255)                     ttCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGca                                                                                                                      <  1:943316/140‑1 (MQ=255)                     ttCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGca                                                                                                                      <  1:600048/140‑1 (MQ=255)                        ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGca                                                                                                                      <  1:311635/137‑1 (MQ=255)                        ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGca                                                                                                                      >  2:311635/1‑137 (MQ=255)                         cGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACAtt                                                                                                                  <  1:209734/140‑1 (MQ=255)                         cGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACAtt                                                                                                                  <  1:1197531/140‑1 (MQ=255)                             cAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGt                                                                                                              <  2:40914/140‑1 (MQ=255)                              aGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTa                                                                                                             <  1:656356/140‑1 (MQ=255)                                     tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGtt                                                                                                     >  2:997045/1‑140 (MQ=255)                                     tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGtt                                                                                                     >  2:396954/1‑140 (MQ=255)                                     tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGtt                                                                                                     >  2:638374/1‑140 (MQ=255)                                     tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGtt                                                                                                     >  1:167234/1‑140 (MQ=255)                                          cTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑‑GGTTAccgcc                                                                                               >  2:687705/1‑140 (MQ=255)                                            aTCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTAccgccg                                                                                              <  1:997045/140‑1 (MQ=255)                                               ttCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCtt                                                                                           >  1:272738/1‑140 (MQ=255)                                                 ccAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑CCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTc                                                                                        >  2:238160/1‑140 (MQ=255)                                                  cAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTAccg                                                                                                 <  2:138181/131‑1 (MQ=255)                                                  cAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTAccg                                                                                                 >  1:138181/1‑131 (MQ=255)                                                    gagTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAg                                                                                      >  1:918497/1‑140 (MQ=255)                                                    gagTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAg                                                                                      <  2:695342/140‑1 (MQ=255)                                                       tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTAccgccg                                                                                              >  1:145687/1‑129 (MQ=255)                                                       tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTAccgccg                                                                                              <  2:145687/129‑1 (MQ=255)                                                         acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCg                                                                                 >  1:919024/1‑140 (MQ=255)                                                         acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCg                                                                                 <  1:785296/140‑1 (MQ=255)                                                         acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCg                                                                                 >  2:785296/1‑140 (MQ=255)                                                                 gCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCCGGCTTTCCCACCCCTTTTGTAATGGGGTAACCCCCCCTTTCAACCACCGCttcccc                                                                          >  2:1071396/1‑136 (MQ=255)                                                                 gCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                                                                         <  1:19045/140‑1 (MQ=255)                                                                 gCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                                                                         <  1:318316/140‑1 (MQ=255)                                                                  cTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCgcgc                                                                        <  2:1117922/140‑1 (MQ=255)                                                                     cAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCa                                                                     <  1:1011851/140‑1 (MQ=255)                                                                     cAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCa                                                                     <  1:590254/140‑1 (MQ=255)                                                                            cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAtt                                                              >  2:488376/1‑140 (MQ=255)                                                                              ttGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTaa                                                            <  2:817392/140‑1 (MQ=255)                                                                               tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAt                                                           >  2:897201/1‑140 (MQ=255)                                                                               tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAt                                                           >  1:106764/1‑140 (MQ=255)                                                                                         ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGt                                                 >  2:76773/1‑140 (MQ=255)                                                                                            gAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTaaa                                              <  1:396954/140‑1 (MQ=255)                                                                                                tCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc                                          <  1:31606/140‑1 (MQ=255)                                                                                                tCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc                                          <  1:663847/140‑1 (MQ=255)                                                                                                tCTTTTTCCTGCTTTTTCCACCCGGTTTGGAATGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTGCCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc                                          <  2:397030/140‑1 (MQ=255)                                                                                                    tttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCAcc                                      <  1:343975/140‑1 (MQ=255)                                                                                                          gCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑CCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGcc                               <  1:238160/140‑1 (MQ=255)                                                                                                           cTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGcc                               <  2:918497/140‑1 (MQ=255)                                                                                                            tttttCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCt                              <  2:70189/140‑1 (MQ=255)                                                                                                            tttttCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCt                              <  2:272738/140‑1 (MQ=255)                                                                                                                    cTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACt                      <  2:106764/140‑1 (MQ=255)                                                                                                                         tttGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTg                 >  1:959873/1‑140 (MQ=255)                                                                                                                                tGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑‑GGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCtt         <  1:687705/140‑1 (MQ=255)                                                                                                                                 gAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCtt         <  1:897201/140‑1 (MQ=255)                                                                                                                                  aGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACt                      <  1:1190654/126‑1 (MQ=255)                                                                                                                                  aGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACt                      >  2:1190654/1‑126 (MQ=255)                                                                                                                                  aGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTc        >  2:670096/1‑140 (MQ=255)                                                                                                                                  aGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTc        >  2:507200/1‑140 (MQ=255)                                                                                                                                      ttGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcg    <  1:638374/140‑1 (MQ=255)                                                                                                                                       tGCAG‑CCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGcgcg  >  1:1076641/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                         AAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑CCCCCCCAGGCTTCCCAGCACATTCTGTAAT‑GGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCGCG  >  NC_000913/3100894‑3101167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑