Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913646,7470TG27.8% 75.6 / 10.1 36T255P (ACC→CCC) citGtriphosphoribosyl‑dephospho‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base T (0/26);  new base G (10/0);  total (10/26)
Fisher's exact test for biased strand distribution p-value = 3.93e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGC  >  NC_000913/646616‑646879
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cAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGc                                                                                                                              <  2:684630/140‑1 (MQ=255)
   gTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGcccc                                                                                                                           <  2:1227094/140‑1 (MQ=255)
    tGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGgcccc                                                                                                                          >  1:1213344/1‑140 (MQ=255)
      cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGcccccct                                                                                                                        >  1:42717/1‑138 (MQ=255)
                tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTTAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCCTTTTTtaataa                                                                                                              >  2:61611/1‑140 (MQ=255)
                        tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTgcg                                                                                                      <  2:790930/140‑1 (MQ=255)
                        tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCCTTTTTCTAAAATAtttttg                                                                                                      >  2:999588/1‑137 (MQ=255)
                                  cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTTGCATATTCCCATTTTAAAATAATTTTTTTCCCTCCCGCTg                                                                                            >  1:1161422/1‑140 (MQ=255)
                                  cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAATGCCCCCCTTTTTCAATAATGTTTTCGCCTCccgccg                                                                                            >  1:111340/1‑138 (MQ=255)
                                    ggTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAATGCCCCCCTTTTTCAATAATATTTTCGCCTCGCGCTGtt                                                                                          >  2:1080917/1‑139 (MQ=255)
                                     gTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGCCCCCCTTTTTTAATAATGTTTTTGCCTCCCGCTGTAg                                                                                         >  2:1218592/1‑140 (MQ=255)
                                      tAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGc                                                                                        <  2:42717/140‑1 (MQ=255)
                                        aGGATCAGTAGGTCAGCACTGCCGCCTGGACTTAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCa                                                                                      <  1:61611/140‑1 (MQ=255)
                                               gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAgg                                                                               <  1:317001/140‑1 (MQ=255)
                                               gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAgg                                                                               <  1:1015668/140‑1 (MQ=255)
                                                     cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGGTTCCCCCCTTTTTTCATAATTTTTTCGCCTCCCCCCGTTGCCCGCGCCGGcccccc                                                                         >  2:866923/1‑140 (MQ=255)
                                                     cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGACCCCCTTTTTTAATAAAATTTTTGCCCCGCGCCGTAGCCAGCGCAGGcccccc                                                                         >  2:1090860/1‑140 (MQ=255)
                                                      aGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCt                                                                        <  1:1054642/140‑1 (MQ=255)
                                                        cACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGCCCCCCTTTTTTAATAATGTTTTTGCCCCCCGCCGTAGCCAGCGCCGGccccccccc                                                                      >  2:1084493/1‑137 (MQ=255)
                                                          cTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATTCCCCCCTTTTTTAATAAATTTTTCGCCCCCCGCTGTTGCCAGCGCCGGccccccccccc                                                                    >  2:219250/1‑135 (MQ=255)
                                                          cTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGGGGGGTGCCCCCCTTTTTTTATAATATTTTGGCCTCCCGCCGTGGCCCGCGCACGccccccccccc                                                                    >  2:317001/1‑135 (MQ=255)
                                                             ccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCCCGCTGTAGCCAGCGCAGGCCCCCCCCGCcccc                                                                 >  2:803939/1‑140 (MQ=255)
                                                                    gACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGAt                                                          <  1:803939/140‑1 (MQ=255)
                                                                     aCTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATg                                                         <  1:927549/140‑1 (MQ=255)
                                                                      cTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGc                                                        <  1:612404/140‑1 (MQ=255)
                                                                      cTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGc                                                        <  1:811315/140‑1 (MQ=255)
                                                                                gttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTa                                              <  1:1216189/140‑1 (MQ=255)
                                                                                gttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTa                                              <  2:645589/140‑1 (MQ=255)
                                                                                           tCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGAt                                   <  2:565528/140‑1 (MQ=255)
                                                                                             ccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCg                                 <  1:999588/140‑1 (MQ=255)
                                                                                                gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGCCCCCCTTTTTTAATAAAGTTTTTGCCCCCCGCCGTGGGCCGGGCAGGCCCCCC‑CCCCCCCCCCCGGCGCAAAGTTGGGATTGCCGCTGGTCTcccc                             >  2:360943/1‑138 (MQ=255)
                                                                                                 tCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt                             <  2:344928/140‑1 (MQ=255)
                                                                                                 tCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt                             <  1:596465/140‑1 (MQ=255)
                                                                                                  cGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGGCCCCCTTTTTTAATAATGTTTTTGCCCCGCGCCGTAGGCAGCGCAGGGCCCCCCCCCCCCCCCGCGGTGCAAAGGTTGGATCGCCCGTGGTTGCCATc                            >  2:205808/1‑140 (MQ=255)
                                                                                                     aCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt                         <  1:124879/140‑1 (MQ=255)
                                                                                                     aCTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATTTTTTCGCCCCCCGCCGTAGCCCGCGCAGGCCCCCCCCCCCCCCCCCCCCTGCCAAGGTGGTATTGCCCGTGATCGCCCTCAGt                         >  2:977550/1‑140 (MQ=255)
                                                                                                     aCTGCCGGAGATAATCGAGATCGGCGCGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt                         <  1:881994/140‑1 (MQ=255)
                                                                                                       tGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg                       <  1:432972/140‑1 (MQ=255)
                                                                                                       tGCCGGAGATAATCGAGATCGGCGGGGGGTTGAATGCCCCCCTTTTTTAATAATTTTTTTGCCCCGCGCGGGAGCCAGCGCCGGGCCCCCCCCCCCCCCCCCGGTGGCACAGTTGTATTGCCGTTGGTTGCCCTCacgag                       >  2:124879/1‑136 (MQ=255)
                                                                                                         ccGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCCTTTTTTAATAAAATTTTTGCCCCCCCCCGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCCCGGTGCAACGTTGGGATCGCCGTTGATCGCCCTCCGTAggg                     >  2:881994/1‑139 (MQ=255)
                                                                                                           ggAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGG‑CCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa                  <  1:360943/140‑1 (MQ=255)
                                                                                                            gagaTAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa                  <  1:808616/140‑1 (MQ=255)
                                                                                                                   tCGAGATCGGCGGGGGGTTGAATGCCCCCCTTTTTTAATAATGTTTTCGCCTCGCGCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGGTGCAACGGTGGGATCGCCGTTTGTCGCCCTCAGTAGGGGGAAGGGAta           >  2:790599/1‑139 (MQ=255)
                                                                                                                            gcggGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc  <  2:259132/140‑1 (MQ=255)
                                                                                                                            gcggGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc  <  1:790599/140‑1 (MQ=255)
                                                                                                                            gcggGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc  <  1:335594/140‑1 (MQ=255)
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CAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGC  >  NC_000913/646616‑646879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑