Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,269,142	A→G	D243G (GAC→GGC)	yhaC →	uncharacterized protein YhaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,269,142	0	A	G	100.0%	317.8 / NA	89	D243G (GAC→GGC)	yhaC	uncharacterized protein YhaC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/47);  total (42/47)

TTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTG  >  NC_000913/3269006‑3269278                                                                                                                                         |                                                                                                                                         tttGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCAt                                                                                                                                       <  1:390739/140‑1 (MQ=255) tttGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCAt                                                                                                                                       <  1:626876/140‑1 (MQ=255)     ttAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTAcc                                                                                                                                   >  2:637035/1‑140 (MQ=255)     ttAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTAcc                                                                                                                                   >  1:604729/1‑140 (MQ=255)     ttAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTAcc                                                                                                                                   >  2:497987/1‑140 (MQ=255)         ttCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCgaag                                                                                                                               <  1:255517/140‑1 (MQ=255)         ttCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCgaag                                                                                                                               >  2:1055468/1‑140 (MQ=255)          tCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCgaaga                                                                                                                              <  1:1112276/140‑1 (MQ=255)          tCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCgaaga                                                                                                                              <  1:11791/140‑1 (MQ=255)                  aaaCGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTaa                                                                                                                      <  2:1126190/140‑1 (MQ=255)                   aaCGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTaaa                                                                                                                     >  1:370873/1‑140 (MQ=255)                   aaCGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTaaa                                                                                                                     >  1:706946/1‑140 (MQ=255)                   aaCGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTaaa                                                                                                                     >  2:1164712/1‑140 (MQ=255)                        cacaATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTa                                                                                                                >  1:1253452/1‑140 (MQ=255)                        cacaATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTa                                                                                                                >  1:942690/1‑140 (MQ=255)                        cacaATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTa                                                                                                                >  2:733661/1‑140 (MQ=255)                             tctcCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTAAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATgaga                                                                                                           >  2:1069160/1‑140 (MQ=255)                                 cTGGAATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTg                                                                                                       <  2:620430/140‑1 (MQ=255)                                   gggATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCa                                                                                                     <  1:649842/140‑1 (MQ=255)                                        tattTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATtat                                                                                                <  1:1075259/140‑1 (MQ=255)                                        tattTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATtat                                                                                                <  1:109508/140‑1 (MQ=255)                                         attTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTCTTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATg                                                                                                              <  2:1126894/125‑1 (MQ=255)                                         attTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATg                                                                                                              >  1:1126894/1‑125 (MQ=255)                                         attTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATtata                                                                                               >  2:840931/1‑140 (MQ=255)                                         attTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATtata                                                                                               >  1:328549/1‑140 (MQ=255)                                         attTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATtata                                                                                               >  1:528487/1‑140 (MQ=255)                                         attTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATtata                                                                                               >  1:52671/1‑140 (MQ=255)                                          tttACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATaa                                                                                              <  1:395075/140‑1 (MQ=255)                                          tttACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATaa                                                                                              <  2:328549/140‑1 (MQ=255)                                              cTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATc                                                                                          <  2:738805/140‑1 (MQ=255)                                               tAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCt                                                                                         >  2:769106/1‑140 (MQ=255)                                               tAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCt                                                                                         >  2:139093/1‑140 (MQ=255)                                                   aaTAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATAc                                                                                     >  2:811241/1‑140 (MQ=255)                                                    aTAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATtata                                                                                               >  1:1260888/1‑129 (MQ=255)                                                    aTAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATtata                                                                                               <  2:1260888/129‑1 (MQ=255)                                                    aTAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATAcc                                                                                    <  2:894911/140‑1 (MQ=255)                                                    aTAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATAcc                                                                                    <  1:840931/140‑1 (MQ=255)                                                    aTAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTACGAGATTTGCAATTATAAAATCTATAcc                                                                                    <  2:468479/140‑1 (MQ=255)                                                        cGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAgg                                                                                <  1:1055468/140‑1 (MQ=255)                                                         gCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAggg                                                                               <  2:52671/140‑1 (MQ=255)                                                          ccTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGc                                                                              <  1:1215211/140‑1 (MQ=255)                                                          ccTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGc                                                                              <  2:470501/140‑1 (MQ=255)                                                          ccTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGc                                                                              <  2:1253452/140‑1 (MQ=255)                                                          ccTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGc                                                                              <  2:942690/140‑1 (MQ=255)                                                           cTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTG‑AATTATAAAATCTATACCAAGGGCaa                                                                            <  1:1204792/140‑1 (MQ=255)                                                            tGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCaa                                                                            <  2:706946/140‑1 (MQ=255)                                                                      aGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGa                                                                  <  1:769106/140‑1 (MQ=255)                                                                       gCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGAt                                                                 <  1:497987/140‑1 (MQ=255)                                                                        cATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATa                                                                >  1:836917/1‑140 (MQ=255)                                                                                atatGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGaa                                                        <  1:139093/140‑1 (MQ=255)                                                                                 tatGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAGTTATGAGATATGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGaaa                                                       >  2:1126901/1‑140 (MQ=255)                                                                                  atGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGaaaa                                                      >  2:990538/1‑140 (MQ=255)                                                                                  atGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGaaaa                                                      >  1:815634/1‑140 (MQ=255)                                                                                   tGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAAt                                                     >  2:797254/1‑140 (MQ=255)                                                                                     tATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGa                                                   >  1:933870/1‑140 (MQ=255)                                                                                     tATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGa                                                   >  1:1106563/1‑140 (MQ=255)                                                                                     tATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGa                                                   <  1:637035/140‑1 (MQ=255)                                                                                        tCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAAtt                                                >  1:691960/1‑140 (MQ=255)                                                                                           gaagaaAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAAc                                             >  2:1119674/1‑140 (MQ=255)                                                                                            aagaaAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACa                                            >  2:66409/1‑140 (MQ=255)                                                                                            aagaaAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACa                                            >  1:335251/1‑140 (MQ=255)                                                                                            aagaaAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACa                                            >  2:468067/1‑140 (MQ=255)                                                                                             agaaAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACaa                                           <  1:468067/140‑1 (MQ=255)                                                                                             agaaAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACaa                                           >  2:587440/1‑140 (MQ=255)                                                                                               aaaaaaTCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACaaaa                                         >  2:300059/1‑140 (MQ=255)                                                                                               aaaaaaTCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACaaaa                                         >  2:998533/1‑140 (MQ=255)                                                                                                aaaaaTCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAg                                        <  2:370873/140‑1 (MQ=255)                                                                                                aaaaaTCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAg                                        <  2:489443/140‑1 (MQ=255)                                                                                                aaaaaTCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAg                                        <  2:604729/140‑1 (MQ=255)                                                                                                 aaaaTCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGa                                       >  1:938582/1‑140 (MQ=255)                                                                                                  aaaTCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGaa                                      <  2:43325/140‑1 (MQ=255)                                                                                                  aaaTCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGaa                                      <  2:836917/140‑1 (MQ=255)                                                                                                   aaTCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGaaa                                     <  1:836539/140‑1 (MQ=255)                                                                                                    aTCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAat                                    <  1:1164712/140‑1 (MQ=255)                                                                                                             gTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAg                           <  2:335251/140‑1 (MQ=255)                                                                                                             gTCGAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAg                           <  1:990538/140‑1 (MQ=255)                                                                                                                gAAAGTTGTTTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTc                        >  1:906414/1‑140 (MQ=255)                                                                                                                    gttgttTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAg                           >  1:391847/1‑133 (MQ=255)                                                                                                                    gttgttTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAg                           <  2:391847/133‑1 (MQ=255)                                                                                                                     ttgttTAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGa                   <  2:111694/140‑1 (MQ=255)                                                                                                                        tttAAATGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGaaaa                >  1:1092096/1‑140 (MQ=255)                                                                                                                              tGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAAtcatca          <  2:938582/140‑1 (MQ=255)                                                                                                                              tGAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAAtcatca          <  2:511464/140‑1 (MQ=255)                                                                                                                               gAGCCTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAA‑TATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAAt        <  2:1032158/140‑1 (MQ=255)                                                                                                                                  ccTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTg      >  2:673451/1‑140 (MQ=255)                                                                                                                                  ccTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTg      >  1:185254/1‑140 (MQ=255)                                                                                                                                  ccTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTg      >  1:1156989/1‑140 (MQ=255)                                                                                                                                   cTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGc     <  2:185254/140‑1 (MQ=255)                                                                                                                                   cTGGAGGCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGc     <  1:340211/140‑1 (MQ=255)                                                                                                                                      gaggCATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTg  >  2:628724/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         TTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTACAGATAAATTAGAAAATGAATTAACAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTG  >  NC_000913/3269006‑3269278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑