Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,401,710	T→C	V541V (GTA→GTG)	csrD ←	regulator of CsrB and CsrC decay

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,401,710	0	T	C	100.0%	225.3 / NA	64	V541V (GTA→GTG)	csrD	regulator of CsrB and CsrC decay
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/31);  total (33/31)

TGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTAACGTATCGCGCAGCC  >  NC_000913/3401573‑3401843                                                                                                                                          |                                                                                                                                      tGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCAc                                                                                                                                     >  2:87392/1‑140 (MQ=255) tGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCAc                                                                                                                                     <  2:5826/140‑1 (MQ=255)   aaCCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCAccc                                                                                                                                   <  1:968062/140‑1 (MQ=255)      cagcagCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTa                                                                                                                                <  1:85503/140‑1 (MQ=255)        gcagcTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTaaa                                                                                                                              >  1:595699/1‑140 (MQ=255)         cagcTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAg                                                                                                                             >  2:607426/1‑140 (MQ=255)                                      ttCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAgg                                                                                                       <  1:496892/133‑1 (MQ=255)                                      ttCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAgg                                                                                                       >  2:496892/1‑133 (MQ=255)                                      ttCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTaaa                                                                                                <  1:1051271/140‑1 (MQ=255)                                       tCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAAc                                                                                               <  2:1025271/140‑1 (MQ=255)                                       tCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAAc                                                                                               <  2:595699/140‑1 (MQ=255)                                        cTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTa                                                                                                  >  2:529377/1‑136 (MQ=255)                                        cTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTa                                                                                                  <  1:529377/136‑1 (MQ=255)                                        cTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACAAATAACAGGTTGTAAACg                                                                                              >  1:320207/1‑140 (MQ=255)                                         tGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAgg                                                                                                       >  2:1248876/1‑130 (MQ=255)                                         tGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAgg                                                                                                       <  1:1248876/130‑1 (MQ=255)                                         tGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGa                                                                                             <  2:23393/140‑1 (MQ=255)                                              aGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGat                                                                                        <  2:472754/140‑1 (MQ=255)                                                ccccGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAAc                                                                                               >  2:434757/1‑131 (MQ=255)                                                ccccGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAAc                                                                                               <  1:434757/131‑1 (MQ=255)                                                 cccGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATg                                                                                     <  2:1199627/140‑1 (MQ=255)                                                 cccGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATg                                                                                     <  2:1155047/140‑1 (MQ=255)                                                     gATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGa                                                                                 >  2:652857/1‑140 (MQ=255)                                                      aTGGAGCATGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGAc                                                                                <  1:929653/140‑1 (MQ=255)                                                          aGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACt                                                                                           <  2:123813/125‑1 (MQ=255)                                                          aGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACt                                                                                           >  1:123813/1‑125 (MQ=255)                                                          aGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGt                                                                                    <  2:384290/132‑1 (MQ=255)                                                          aGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGt                                                                                    >  2:920814/1‑132 (MQ=255)                                                          aGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGt                                                                                    >  1:384290/1‑132 (MQ=255)                                                          aGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGt                                                                                    <  1:920814/132‑1 (MQ=255)                                                          aGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTAc                                                                            >  1:146755/1‑140 (MQ=255)                                                               gAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCgg                                                                       >  2:87491/1‑140 (MQ=255)                                                                aGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGc                                                                      <  1:652857/140‑1 (MQ=255)                                                                aGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGc                                                                      >  2:12713/1‑140 (MQ=255)                                                                    aCTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCctct                                                                  >  1:765254/1‑140 (MQ=255)                                                                    aCTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCctct                                                                  >  1:1217088/1‑140 (MQ=255)                                                                     cTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTg                                                                 >  1:901807/1‑140 (MQ=255)                                                                     cTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTg                                                                 >  2:450223/1‑140 (MQ=255)                                                                      tCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGc                                                                <  2:901807/140‑1 (MQ=255)                                                                      tCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGc                                                                <  1:450223/140‑1 (MQ=255)                                                                          cATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGt                                                            >  2:800807/1‑140 (MQ=255)                                                                             tAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCa                                                         <  1:600973/140‑1 (MQ=255)                                                                               aGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGc                                                                >  2:708426/1‑131 (MQ=255)                                                                               aGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGc                                                                <  1:708426/131‑1 (MQ=255)                                                                               aGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCa                                                         >  2:412025/1‑138 (MQ=255)                                                                               aGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCa                                                         <  1:412025/138‑1 (MQ=255)                                                                                 ttCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCaataa                                                     <  1:607426/140‑1 (MQ=255)                                                                                         tCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGt                                             >  2:1075064/1‑140 (MQ=255)                                                                                             aCTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTc                                         >  1:233087/1‑140 (MQ=255)                                                                                                   aCTTACCAGCGTCAGACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTtgtg                                   >  2:697418/1‑140 (MQ=255)                                                                                                         cAGCGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGAttttt                             >  1:630892/1‑140 (MQ=255)                                                                                                            cGTCAGACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTcac                          <  1:697418/140‑1 (MQ=255)                                                                                                            cGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTcac                          >  2:231233/1‑140 (MQ=255)                                                                                                            cGTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTcac                          >  2:1175388/1‑140 (MQ=255)                                                                                                             gTCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTcaca                         <  2:233087/140‑1 (MQ=255)                                                                                                              tCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACAt                        >  1:998548/1‑140 (MQ=255)                                                                                                              tCAAACCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACAt                        >  1:986134/1‑140 (MQ=255)                                                                                                                  aCCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCa                    <  2:1089473/140‑1 (MQ=255)                                                                                                                  aCCAGCCTGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCa                    >  1:1089473/1‑140 (MQ=255)                                                                                                                         tGGTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTAACGt             <  2:146755/140‑1 (MQ=255)                                                                                                                          ggTTGACGGCTACCCGCACCCCTAAAGCATTCACTAAACAAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTAACGTa            <  2:320207/140‑1 (MQ=255)                                                                                                                            ttGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTAACGTATc          >  2:1154884/1‑140 (MQ=255)                                                                                                                            ttGACGGCTACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTAACGTATc          >  2:1044131/1‑140 (MQ=255)                                                                                                                                    tACCCGCACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTAACGTATCGCGCAGcc  <  2:1217088/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TGAACCAGCAGCTGGTTCTCCGTTCGCTTCTCAATGTTTCTGACCAGCCCCGGATGGAGCTTGAGTAACTCAACATTAAGTTCTTTGATCCAACTGGTACTTACCAGCGTCAAACCAGCCTGGTTGACGGCTACCCGTACCCCTAAAGCATTCACTAAACGAATAACAGGTTGTAAACGACTGATATGTTGACCTACATCGGCCTCTGCAAGTTCAATAATTATGCGTTTTCGTTGTGATTTTTCACATTGCATTAACGTATCGCGCAGCC  >  NC_000913/3401573‑3401843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑