Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,481,078	T→C	K28K (AAA→AAG)	kefG ←	protein required for KefB activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,481,078	0	T	C	100.0%	283.0 / NA	80	K28K (AAA→AAG)	kefG	protein required for KefB activity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/39);  total (41/39)

GTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCAT  >  NC_000913/3480944‑3481210                                                                                                                                       |                                                                                                                                     gTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAg                                                                                                                                 <  2:12070/140‑1 (MQ=255)   ataAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCa                                                                                                                               <  1:951193/140‑1 (MQ=255)     aaaGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGt                                                                                                                             >  1:426956/1‑140 (MQ=255)     aaaGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGt                                                                                                                             >  1:281323/1‑140 (MQ=255)     aaaGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCAC‑GGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTaa                                                                                                                                  <  2:328867/134‑1 (MQ=255)     aaaGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCAC‑GGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTaa                                                                                                                                  >  1:328867/1‑134 (MQ=255)      aaGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTa                                                                                                                            <  1:1211273/140‑1 (MQ=255)         agGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTAccc                                                                                                                         >  1:409101/1‑140 (MQ=255)          ggATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCg                                                                                                                        >  1:646032/1‑140 (MQ=255)          ggATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCg                                                                                                                        <  1:431145/140‑1 (MQ=255)           gATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAGAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCgg                                                                                                                       >  2:1186469/1‑140 (MQ=255)           gATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCgg                                                                                                                       >  1:57954/1‑140 (MQ=255)           gATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCgg                                                                                                                       >  1:861181/1‑140 (MQ=255)           gATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCgg                                                                                                                       >  1:1222067/1‑140 (MQ=255)           gATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCgg                                                                                                                       >  1:43101/1‑140 (MQ=255)            aTGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGt                                                                                                                      >  2:957257/1‑140 (MQ=255)                   aaGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCAc                                                                                                               >  1:425223/1‑140 (MQ=255)                   aaGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCAc                                                                                                               <  2:1109257/140‑1 (MQ=255)                    aGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCAcc                                                                                                              <  1:75864/140‑1 (MQ=255)                     gACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCg                                                                                                             >  1:802276/1‑140 (MQ=255)                         aTCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTc                                                                                                         >  1:269407/1‑140 (MQ=255)                           cACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCt                                                                                                       <  1:35446/140‑1 (MQ=255)                            aCCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTg                                                                                                      <  2:281323/140‑1 (MQ=255)                                  tGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTc                                                                                                <  2:460300/140‑1 (MQ=255)                                  tGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTc                                                                                                >  1:728225/1‑140 (MQ=255)                                      cgcgCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGa                                                                                            <  1:865072/140‑1 (MQ=255)                                       gcgcAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGAt                                                                                           <  2:437711/140‑1 (MQ=255)                                               aTGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATAc                                                                                   >  1:323702/1‑140 (MQ=255)                                                       tCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCaa                                                                           >  1:500203/1‑140 (MQ=255)                                                       tCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCaa                                                                           >  1:881337/1‑140 (MQ=255)                                                       tCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCaa                                                                           >  1:35792/1‑140 (MQ=255)                                                       tCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCaa                                                                           >  1:61971/1‑140 (MQ=255)                                                         aCGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCaaaa                                                                         >  2:999348/1‑140 (MQ=255)                                                                 tatCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCgct                                                                 >  2:664341/1‑140 (MQ=255)                                                                 tatCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCgct                                                                 <  2:861181/140‑1 (MQ=255)                                                                  atCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCgctg                                                                >  2:249504/1‑140 (MQ=255)                                                                   tCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCgctgg                                                               >  1:515517/1‑140 (MQ=255)                                                                   tCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCgctgg                                                               <  2:447452/140‑1 (MQ=255)                                                                     aaTAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCgctggct                                                             <  2:1069987/140‑1 (MQ=255)                                                                      aTAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCgctggctg                                                            <  1:999348/140‑1 (MQ=255)                                                                       tAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTga                                                           <  1:957257/140‑1 (MQ=255)                                                                       tAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTCGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTga                                                           >  1:867520/1‑140 (MQ=255)                                                                        aaaaaaaTCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTgag                                                          <  2:1240295/140‑1 (MQ=255)                                                                         aaaaaaTCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTgaga                                                         <  2:37860/140‑1 (MQ=255)                                                                           aaaaTCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGAca                                                       <  1:1186469/140‑1 (MQ=255)                                                                               tCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCg                                                                   >  1:205475/1‑124 (MQ=255)                                                                               tCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCg                                                                   <  2:205475/124‑1 (MQ=255)                                                                               tCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTCGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGAcatcat                                                   <  2:867520/140‑1 (MQ=255)                                                                                  ggATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATccc                                                >  2:677471/1‑140 (MQ=255)                                                                                    aTAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATccccc                                              <  2:515517/140‑1 (MQ=255)                                                                                     tAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCt                                             <  1:677471/140‑1 (MQ=255)                                                                                      aGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTc                                            <  2:426956/140‑1 (MQ=255)                                                                                          cgcgTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCgg                                        <  2:1222067/140‑1 (MQ=255)                                                                                           gcgTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTA‑CCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTa                                      <  1:1034152/140‑1 (MQ=255)                                                                                            cgTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTa                                      <  1:664341/140‑1 (MQ=255)                                                                                            cgTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTa                                      <  1:249504/140‑1 (MQ=255)                                                                                            cgTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTa                                      >  2:342326/1‑140 (MQ=255)                                                                                             gTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTAt                                     <  2:1014323/140‑1 (MQ=255)                                                                                             gTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTAt                                     >  1:1261456/1‑140 (MQ=255)                                                                                             gTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTAt                                     >  2:843668/1‑140 (MQ=255)                                                                                             gTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTAt                                     <  2:881337/140‑1 (MQ=255)                                                                                               aaaGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATgg                                   <  2:1261456/140‑1 (MQ=255)                                                                                                 aGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGtt                                 <  2:425223/140‑1 (MQ=255)                                                                                                  ggTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGttt                                <  2:57954/140‑1 (MQ=255)                                                                                                  ggTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGttt                                >  1:1165550/1‑140 (MQ=255)                                                                                                              tAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTc                    >  1:46544/1‑140 (MQ=255)                                                                                                               aaCATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCg                   <  1:342326/140‑1 (MQ=255)                                                                                                                  aTTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTg                <  2:728225/140‑1 (MQ=255)                                                                                                                     gCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGttt             <  1:315925/140‑1 (MQ=255)                                                                                                                     gCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGttt             <  1:610813/140‑1 (MQ=255)                                                                                                                      cTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGtttt            >  1:483018/1‑140 (MQ=255)                                                                                                                      cTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGtttt            >  1:261312/1‑140 (MQ=255)                                                                                                                       tGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTg           <  2:1165550/140‑1 (MQ=255)                                                                                                                        gAGCTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTgg          >  2:149491/1‑140 (MQ=255)                                                                                                                           cTGCGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCt       >  1:602455/1‑140 (MQ=255)                                                                                                                              cGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTAcc    >  2:58433/1‑140 (MQ=255)                                                                                                                              cGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTAcc    >  1:143028/1‑140 (MQ=255)                                                                                                                              cGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTAcc    >  1:530578/1‑140 (MQ=255)                                                                                                                              cGTGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTAcc    >  1:1110774/1‑140 (MQ=255)                                                                                                                                tGGCCGGCTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCAt  >  1:1220313/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     GTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCAT  >  NC_000913/3480944‑3481210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑