Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,551,477	C→T	A333T (GCC→ACC)	malP ←	maltodextrin phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,551,477	0	C	T	100.0%	230.1 / NA	66	A333T (GCC→ACC)	malP	maltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/31);  total (35/31)

CGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGCGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGAT  >  NC_000913/3551341‑3551611                                                                                                                                         |                                                                                                                                       cGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTc                                                                                                                                     >  2:333830/1‑140 (MQ=255)   aGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCAt                                                                                                                                   >  1:557128/1‑140 (MQ=255)   aGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCAt                                                                                                                                   >  2:752928/1‑140 (MQ=255)      aTTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATccc                                                                                                                                <  2:94465/140‑1 (MQ=255)        tAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAg                                                                                                                              <  1:124447/140‑1 (MQ=255)        tAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAg                                                                                                                              <  2:762372/140‑1 (MQ=255)               gTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCt                                                                                                                       >  1:385569/1‑140 (MQ=255)               gTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCt                                                                                                                       >  2:497927/1‑140 (MQ=255)                 taataaTCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTgg                                                                                                                     >  1:841067/1‑140 (MQ=255)                  aataatCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGt                                                                                                                    <  2:614256/140‑1 (MQ=255)                  aataatCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGt                                                                                                                    <  1:333830/140‑1 (MQ=255)                           cATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCt                                                                                                                       >  1:1030215/1‑128 (MQ=255)                           cATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCt                                                                                                                       <  2:1030215/128‑1 (MQ=255)                               tgGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAg                                                                                                       <  1:277551/140‑1 (MQ=255)                                 gcgcGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCa                                                                                                     >  1:598096/1‑140 (MQ=255)                                        aGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCgcag                                                                                              <  2:598096/140‑1 (MQ=255)                                        aGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCgcag                                                                                              <  2:151688/140‑1 (MQ=255)                                        aGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCgcag                                                                                              <  1:752928/140‑1 (MQ=255)                                        aGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCgcag                                                                                              <  1:1060369/140‑1 (MQ=255)                                          tAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCgcagca                                                                                            >  1:1134768/1‑140 (MQ=255)                                           aaGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGcagcag                                                                                           <  1:267094/140‑1 (MQ=255)                                                 ttCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTgg                                                                                     <  2:385569/140‑1 (MQ=255)                                                  tCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGa                                                                                    >  1:959286/1‑140 (MQ=255)                                                      cAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATcg                                                                                <  2:557128/140‑1 (MQ=255)                                                      cAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATcg                                                                                <  2:959286/140‑1 (MQ=255)                                                        gTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATcgcg                                                                              >  2:106051/1‑140 (MQ=255)                                                        gTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATcgcg                                                                              >  2:1003992/1‑140 (MQ=255)                                                          ttCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGAt                                                                            <  1:846995/140‑1 (MQ=255)                                                           tCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATa                                                                           >  1:1179664/1‑140 (MQ=255)                                                             acaTCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGt                                                                         <  2:841067/140‑1 (MQ=255)                                                                  ccAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTtgggt                                                                    >  1:562196/1‑140 (MQ=255)                                                                  ccAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTtgggt                                                                    <  1:1003992/140‑1 (MQ=255)                                                                   cAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTtgggtg                                                                   >  2:1065754/1‑140 (MQ=255)                                                                   cAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTtgggtg                                                                   >  1:874594/1‑140 (MQ=255)                                                                   cAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTtgggtg                                                                   >  1:438540/1‑140 (MQ=255)                                                                   cAGCGTCCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTtgggtg                                                                   >  1:223029/1‑140 (MQ=255)                                                                     gCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGt                                                                         <  1:1225499/132‑1 (MQ=255)                                                                     gCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGt                                                                         >  2:1225499/1‑132 (MQ=255)                                                                         tCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATc                                                             >  1:1082048/1‑140 (MQ=255)                                                                             gcgcTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTc                                                         <  2:1134768/140‑1 (MQ=255)                                                                               gcTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAg                                                       <  2:1179664/140‑1 (MQ=255)                                                                                    tGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGaa                                                  <  2:562196/140‑1 (MQ=255)                                                                                      gCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATa                                                <  1:618350/140‑1 (MQ=255)                                                                                           aGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATaa                                               <  1:518474/136‑1 (MQ=255)                                                                                           aGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATaa                                               >  2:518474/1‑136 (MQ=255)                                                                                                aTGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAAt                                      >  1:284334/1‑140 (MQ=255)                                                                                                       gtgtAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAg                               >  2:871259/1‑140 (MQ=255)                                                                                                       gtgtAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAg                               >  2:724331/1‑140 (MQ=255)                                                                                                       gtgtAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAg                               >  1:344642/1‑140 (MQ=255)                                                                                                        tgtAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGt                              <  1:157632/140‑1 (MQ=255)                                                                                                         gtAAGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGtt                             >  1:1150548/1‑140 (MQ=255)                                                                                                            aGCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGt                          >  2:1147976/1‑140 (MQ=255)                                                                                                             gCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTg                         >  1:307274/1‑140 (MQ=255)                                                                                                             gCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTg                         <  1:497927/140‑1 (MQ=255)                                                                                                             gCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTg                         >  2:971291/1‑140 (MQ=255)                                                                                                             gCGAAAGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTg                         >  1:135324/1‑140 (MQ=255)                                                                                                                  aGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGt                          >  2:470877/1‑134 (MQ=255)                                                                                                                  aGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGt                          <  2:873374/134‑1 (MQ=255)                                                                                                                  aGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGt                          <  1:470877/134‑1 (MQ=255)                                                                                                                  aGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGtt                    >  1:498049/1‑140 (MQ=255)                                                                                                                  aGTTTTGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTAAATAACTTCGTAATCCGCCAGTTCGt                          >  1:873374/1‑134 (MQ=255)                                                                                                                       tGCTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGc               <  1:871259/140‑1 (MQ=255)                                                                                                                         cTGGTAATGGCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGccc             <  2:284334/140‑1 (MQ=255)                                                                                                                                  gCCCAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAgatg    <  2:1150548/140‑1 (MQ=255)                                                                                                                                   cccAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAgatga   >  2:303570/1‑140 (MQ=255)                                                                                                                                    ccAGGTGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAgatgat  >  2:487563/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CGAGTATTAATTTCGTTAATAATCTGCATGTGGCGCGGCAGTAAGCCTTTCACCAGTTTCACATCCCAGCGTTCCAGCGCTTCTGGCATCAGGGTATGGTTGGTGTAAGCGAAAGTTTTGCTGGTAATGGCCCAGGCGTCATCCCAGCTCATCTGGTGCTCATCGATCAGCACGCGCAGCAGTTCTGGAATCGCGATAGTTGGGTGGGTATCGTTCAGCTGAATAACTTCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGAT  >  NC_000913/3551341‑3551611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑