Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,660,928	T→C	V172A (GTT→GCT)	mdtF →	multidrug efflux pump RND permease MdtF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,660,928	0	T	C	100.0%	260.5 / NA	74	V172A (GTT→GCT)	mdtF	multidrug efflux pump RND permease MdtF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/37);  total (37/37)

AGCGTCGATAAGTCGAGCAGTAATATCCTGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGTTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGATTAAGGTGCAAAACAACCAGATTTCCGGTGG  >  NC_000913/3660795‑3661063                                                                                                                                      |                                                                                                                                        aGCGTCGATAAGTCGAGCAGTAATATCCTGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAg                                                                                                                                   >  1:1248584/1‑140 (MQ=255)     tCGATAAGTCGAGCAGTAATATCCTGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTa                                                                                                                               <  1:1012234/140‑1 (MQ=255)            gTCGAGCAGTAATATCCTGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCg                                                                                                                                 <  2:518835/131‑1 (MQ=255)            gTCGAGCAGTAATATCCTGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCg                                                                                                                                 >  1:518835/1‑131 (MQ=255)            gTCGAGCAGTAATATCCTGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAActct                                                                                                                        <  1:95474/140‑1 (MQ=255)             tCGAGCAGTAATATCCTGATGGTAGCGGCGTTTATTTCTGATAACGGTAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCtt                                                                                                                       <  2:270073/140‑1 (MQ=255)                 gCAGTAATATCCTGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTa                                                                                                                               <  2:622964/128‑1 (MQ=255)                 gCAGTAATATCCTGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTa                                                                                                                               >  1:622964/1‑128 (MQ=255)                 gCAGTAATATCCTGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGt                                                                                                                   <  1:572912/140‑1 (MQ=255)                   aGTAATATCCTGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTc                                                                                                                 <  2:1257344/140‑1 (MQ=255)                       atatCCTGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGa                                                                                                              <  1:1217416/139‑1 (MQ=255)                       atatCCTGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGa                                                                                                              >  2:1217416/1‑139 (MQ=255)                           ccTGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATg                                                                                                         <  1:441266/140‑1 (MQ=255)                             tGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGcc                                                                                                       >  1:94766/1‑140 (MQ=255)                               aTGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTc                                                                                                                 >  1:1007080/1‑128 (MQ=255)                               aTGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTc                                                                                                                 <  2:1007080/128‑1 (MQ=255)                               aTGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCAt                                                                                                     >  2:569541/1‑140 (MQ=255)                               aTGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCAt                                                                                                     >  2:186219/1‑140 (MQ=255)                               aTGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCAt                                                                                                     >  2:165162/1‑140 (MQ=255)                               aTGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCAt                                                                                                     >  1:230825/1‑140 (MQ=255)                               aTGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCAt                                                                                                     >  2:826301/1‑140 (MQ=255)                               aTGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCAt                                                                                                     >  1:227385/1‑140 (MQ=255)                                   tAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGt                                                                                                 >  1:923844/1‑140 (MQ=255)                                    aGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTa                                                                                                >  2:196627/1‑140 (MQ=255)                                     gcggcgTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTAt                                                                                               <  2:433745/140‑1 (MQ=255)                                      cggcgTTTATTTCTGATAACGGCAGCCTCAACCANTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATc                                                                                              >  1:162611/1‑140 (MQ=255)                                             tATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATctggctgg                                                                                       >  2:624441/1‑140 (MQ=255)                                                       aaCGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCaaaa                                                                             <  2:227385/140‑1 (MQ=255)                                                        aCGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCaaaaa                                                                            <  1:165162/140‑1 (MQ=255)                                                            cAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCa                                                                        <  1:561184/140‑1 (MQ=255)                                                             aGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaa                                                                       <  2:94766/140‑1 (MQ=255)                                                             aGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaa                                                                       <  1:186219/140‑1 (MQ=255)                                                             aGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaa                                                                       <  1:196627/140‑1 (MQ=255)                                                             aGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaa                                                                       <  2:923844/140‑1 (MQ=255)                                                              gCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaat                                                                      <  2:230825/140‑1 (MQ=255)                                                              gCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaat                                                                      <  1:461698/140‑1 (MQ=255)                                                                   aaCCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaataaata                                                                 <  1:618714/140‑1 (MQ=255)                                                                   aaCCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaataaata                                                                 <  2:874978/140‑1 (MQ=255)                                                                   aaCCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaataaata                                                                 <  2:744275/140‑1 (MQ=255)                                                                   aaCCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaataaata                                                                 <  2:1248584/140‑1 (MQ=255)                                                                   aaCCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaataaata                                                                 <  2:765681/140‑1 (MQ=255)                                                                   aaCCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaataaata                                                                 <  2:808925/140‑1 (MQ=255)                                                                   aaCCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaataaata                                                                 <  1:826301/140‑1 (MQ=255)                                                                         tACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaa                                                                       >  2:502267/1‑128 (MQ=255)                                                                         tACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCaa                                                                       <  1:502267/128‑1 (MQ=255)                                                                         tACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCt                                                           >  2:1178652/1‑140 (MQ=255)                                                                                     gACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGa                                               <  1:624441/140‑1 (MQ=255)                                                                                          tGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTc                                                  <  2:1006271/132‑1 (MQ=255)                                                                                          tGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTc                                                  >  1:1006271/1‑132 (MQ=255)                                                                                          tGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTa                                          >  1:3916/1‑140 (MQ=255)                                                                                          tGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTa                                          <  1:1178652/140‑1 (MQ=255)                                                                                                 tCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCa                                   <  1:569541/140‑1 (MQ=255)                                                                                                  cTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAg                                  >  2:638871/1‑140 (MQ=255)                                                                                                    aaTATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTc                                      >  1:441535/1‑134 (MQ=255)                                                                                                    aaTATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTc                                      <  2:441535/134‑1 (MQ=255)                                                                                                    aaTATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGAt                                >  2:280821/1‑140 (MQ=255)                                                                                                    aaTATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGAt                                >  1:1236956/1‑140 (MQ=255)                                                                                                    aaTATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGAt                                >  2:268167/1‑140 (MQ=255)                                                                                                    aaTATCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGAt                                >  2:885335/1‑140 (MQ=255)                                                                                                      tatCAAAGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGATTa                              <  2:162611/140‑1 (MQ=255)                                                                                                           aaGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGATTAAGGTg                         >  2:604478/1‑140 (MQ=255)                                                                                                           aaGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGATTAAGGTg                         >  2:856910/1‑140 (MQ=255)                                                                                                           aaGACCCGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGATTAAGGTg                         >  1:586950/1‑140 (MQ=255)                                                                                                               cccGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTcc                                     >  2:304595/1‑124 (MQ=255)                                                                                                               cccGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTcc                                     <  1:304595/124‑1 (MQ=255)                                                                                                               cccGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTc                                      >  2:770448/1‑123 (MQ=255)                                                                                                               cccGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTc                                      <  1:770448/123‑1 (MQ=255)                                                                                                               cccGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGATTAAGGTGCaaa                     >  2:563256/1‑140 (MQ=255)                                                                                                                 cGCTAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGATTAAGGTGCAAaac                   >  2:932331/1‑140 (MQ=255)                                                                                                                    tAAGCCGTACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGATTAAGGTGCAAaacaac                <  2:1236956/140‑1 (MQ=255)                                                                                                                           tACCGCGGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGATTAAGGTGCAAAACAACCAGAttt         >  2:1028820/1‑140 (MQ=255)                                                                                                                                cgGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGATTAAGGTGCAAAACAACCAGATTTCCggt    >  2:204655/1‑140 (MQ=255)                                                                                                                                cgGGCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGATTAAGGTGCAAAACAACCAGATTTCCggt    >  1:434041/1‑140 (MQ=255)                                                                                                                                  ggCGCTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGATTAAGGTGCAAAACAACCAGATTTCCggtgg  >  2:248086/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        AGCGTCGATAAGTCGAGCAGTAATATCCTGATGGTAGCGGCGTTTATTTCTGATAACGGCAGCCTCAACCAGTACGATATCGCGGACTATGTAGCGTCTAATATCAAAGACCCGCTAAGCCGTACCGCGGGCGTTGGTAGCGTACAACTCTTTGGTTCCGAGTATGCCATGCGTATCTGGCTGGACCCGCAAAAACTCAATAAATATAACCTGGTACCTTCCGATGTTATTTCCCAGATTAAGGTGCAAAACAACCAGATTTCCGGTGG  >  NC_000913/3660795‑3661063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑