Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,965,878	G→A	A40T (GCC→ACC)	trxA →	thioredoxin 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,965,878	0	G	A	98.8%	273.2 / ‑5.9	82	A40T (GCC→ACC)	trxA	thioredoxin 1
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (42/39);  total (42/40)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTCCTGTGGAGTTATATATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAA  >  NC_000913/3965742‑3966011                                                                                                                                         |                                                                                                                                      tATTCCTGTGGAGTTATATATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCAccc                                                                                                                                    <  2:249726/140‑1 (MQ=255) tATTCCTGTGGAGTTATATATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCAccc                                                                                                                                    >  1:390390/1‑140 (MQ=255)   ttCCTGTGGAGTTATATATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCg                                                                                                                                  >  2:288350/1‑140 (MQ=255)        gtgGAGTTATATATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCt                                                                                                                             >  1:1059454/1‑140 (MQ=255)         tgGAGTTATATATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTg                                                                                                                            >  2:303859/1‑140 (MQ=255)         tgGAGTTATATATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTg                                                                                                                            >  2:358670/1‑140 (MQ=255)         tgGAGTTATATATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTg                                                                                                                            >  2:594640/1‑140 (MQ=255)                 tatatGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAAt                                                                                                                    >  1:169111/1‑140 (MQ=255)                    atGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGc                                                                                                                 <  2:42593/140‑1 (MQ=255)                    atGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGc                                                                                                                 <  1:594640/140‑1 (MQ=255)                    atGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGc                                                                                                                 <  1:86201/140‑1 (MQ=255)                      gAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACTCCGATTCTGGATGAAATCGCTg                                                                                                               >  1:153365/1‑140 (MQ=255)                      gAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGa                                                                                                                                 >  2:642640/1‑122 (MQ=255)                      gAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGa                                                                                                                                 <  1:642640/122‑1 (MQ=255)                      gAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTg                                                                                                               <  1:303859/140‑1 (MQ=255)                      gAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTg                                                                                                               >  2:375428/1‑140 (MQ=255)                      gAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTg                                                                                                               <  1:338944/140‑1 (MQ=255)                      gAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTg                                                                                                               >  2:944373/1‑140 (MQ=255)                       aGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGa                                                                                                              >  2:716976/1‑140 (MQ=255)                        gCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGc                                                                                                                 >  1:871915/1‑136 (MQ=255)                        gCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGc                                                                                                                 <  2:871915/136‑1 (MQ=255)                        gCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGAc                                                                                                             >  2:1213500/1‑140 (MQ=255)                        gCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGAc                                                                                                             >  2:1134076/1‑140 (MQ=255)                         cGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGaa                                                                                                                      >  1:610834/1‑130 (MQ=255)                         cGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGaa                                                                                                                      <  2:610834/130‑1 (MQ=255)                           aTAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGaa                                                                                                          >  2:884101/1‑140 (MQ=255)                           aTAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGaa                                                                                                          >  2:907539/1‑140 (MQ=255)                           aTAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGaa                                                                                                          >  1:2846/1‑140 (MQ=255)                           aTAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGaa                                                                                                          >  1:342301/1‑140 (MQ=255)                           aTAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGaa                                                                                                          >  2:848070/1‑140 (MQ=255)                           aTAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCAATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGaa                                                                                                          >  1:1213786/1‑140 (MQ=255)                              aaaTTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAatat                                                                                                       <  1:907539/140‑1 (MQ=255)                                         ctgactgaCGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACt                                                                                            <  1:358670/140‑1 (MQ=255)                                          tgactgacGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTg                                                                                           >  2:754109/1‑140 (MQ=255)                                            actgacGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGAc                                                                                         >  1:1077369/1‑140 (MQ=255)                                            actgacGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGAc                                                                                         >  1:333013/1‑140 (MQ=255)                                            actgacGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGAc                                                                                         <  2:393275/140‑1 (MQ=255)                                            actgacGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGAc                                                                                         >  2:328998/1‑140 (MQ=255)                                                acgacAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGtt                                                                                     >  1:204113/1‑140 (MQ=255)                                                 cgacAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTg                                                                                    <  1:604754/140‑1 (MQ=255)                                                 cgacAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTg                                                                                    <  1:288350/140‑1 (MQ=255)                                                   acAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCa                                                                                  <  1:1213500/140‑1 (MQ=255)                                                   acAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCa                                                                                  <  1:884101/140‑1 (MQ=255)                                                   acAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCa                                                                                  <  1:754109/140‑1 (MQ=255)                                                   acAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCa                                                                                  <  1:375428/140‑1 (MQ=255)                                                                 gATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGa                                                                    >  2:1017858/1‑140 (MQ=255)                                                                  aTGTACTCAAAGCGGACGGGGCGATCCTCGTCAATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGAt                                                                   <  2:1213786/140‑1 (MQ=255)                                                                     tACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCaa                                                                <  2:78943/140‑1 (MQ=255)                                                                     tACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCaa                                                                <  1:1024512/140‑1 (MQ=255)                                                                     tACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCaa                                                                <  1:929822/140‑1 (MQ=255)                                                                     tACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCaa                                                                <  2:342301/140‑1 (MQ=255)                                                                     tACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCCGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCaa                                                                >  1:232037/1‑140 (MQ=255)                                                                           aaGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCt                                                          >  1:883001/1‑140 (MQ=255)                                                                            aGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTg                                                         >  1:132802/1‑140 (MQ=255)                                                                            aGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTg                                                         >  1:991298/1‑140 (MQ=255)                                                                             gCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTgg                                                        <  1:328998/140‑1 (MQ=255)                                                                               ggACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCa                                                      >  2:1145821/1‑140 (MQ=255)                                                                               ggACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCa                                                      <  1:944373/140‑1 (MQ=255)                                                                                gACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCAc                                                     >  1:604143/1‑140 (MQ=255)                                                                                  cGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTg                                                   <  1:716976/140‑1 (MQ=255)                                                                                    gggCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTgcg                                                 <  2:68799/140‑1 (MQ=255)                                                                                    gggCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTgcg                                                 >  1:717501/1‑140 (MQ=255)                                                                                     ggCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTgcgc                                                <  2:390390/140‑1 (MQ=255)                                                                                      gCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGcc                                               <  2:883001/140‑1 (MQ=255)                                                                                        gATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGa                                             <  1:848070/140‑1 (MQ=255)                                                                                          tCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGaaa                                           <  2:904469/140‑1 (MQ=255)                                                                                            cTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAata                                         <  2:204113/140‑1 (MQ=255)                                                                                             tcgtcgATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAatat                                        <  2:333013/140‑1 (MQ=255)                                                                                                        tGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTgg                             <  2:232037/140‑1 (MQ=255)                                                                                                              gagTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATccc                       <  2:132802/140‑1 (MQ=255)                                                                                                               agTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCg                      >  1:937887/1‑140 (MQ=255)                                                                                                               agTGGTGCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCg                      >  1:853746/1‑140 (MQ=255)                                                                                                                gtggtgCGGTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGa                     <  1:981404/140‑1 (MQ=255)                                                                                                                        gTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTctgctg             <  2:996906/140‑1 (MQ=255)                                                                                                                        gTCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTctgctg             <  2:169111/140‑1 (MQ=255)                                                                                                                         tCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTctgctgc            >  2:521016/1‑140 (MQ=255)                                                                                                                         tCCGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTctgctgc            >  2:1198475/1‑140 (MQ=255)                                                                                                                           cGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATccc                       <  1:557476/127‑1 (MQ=255)                                                                                                                           cGTGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATccc                       >  2:557476/1‑127 (MQ=255)                                                                                                                             tGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGtt        >  2:810765/1‑140 (MQ=255)                                                                                                                             tGCAAAATGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGtt        >  2:623158/1‑140 (MQ=255)                                                                                                                                   aTGATCACCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCaaaaa  <  2:1077369/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TATTCCTGTGGAGTTATATATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAA  >  NC_000913/3965742‑3966011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑