Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	478,313	A→G	V102A (GTT→GCT)	pdeB ←	c‑di‑GMP phosphodiesterase PdeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	478,313	0	A	G	100.0%	338.2 / NA	95	V102A (GTT→GCT)	pdeB	c‑di‑GMP phosphodiesterase PdeB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/49);  total (46/49)

CATGGCGACCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGT  >  NC_000913/478177‑478449                                                                                                                                         |                                                                                                                                         cATGGCGACCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCgt                                                                                                                                       <  2:964429/140‑1 (MQ=255)  aTGGCGACCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCgtt                                                                                                                                      >  2:1136732/1‑140 (MQ=255)       gACCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTAt                                                                                                                                 <  1:997700/140‑1 (MQ=255)       gACCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTAt                                                                                                                                 <  2:817699/140‑1 (MQ=255)       gACCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTAt                                                                                                                                 <  2:1196489/140‑1 (MQ=255)       gACCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTAt                                                                                                                                 <  2:467400/140‑1 (MQ=255)        aCCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATc                                                                                                                                >  1:560598/1‑140 (MQ=255)        aCCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATc                                                                                                                                >  2:439430/1‑140 (MQ=255)        aCCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATc                                                                                                                                >  2:604275/1‑140 (MQ=255)          cATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTAt                                                                                                                                 >  2:415306/1‑137 (MQ=255)          cATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTAt                                                                                                                                 <  1:415306/137‑1 (MQ=255)          cATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGa                                                                                                                              >  1:989282/1‑140 (MQ=255)                  ggATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGcc                                                                                                                      <  1:158818/140‑1 (MQ=255)                    aTAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCgtt                                                                                                                                      <  1:166682/122‑1 (MQ=255)                    aTAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCgtt                                                                                                                                      >  2:166682/1‑122 (MQ=255)                    aTAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCAc                                                                                                                    <  2:1141855/140‑1 (MQ=255)                    aTAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCAc                                                                                                                    >  2:789143/1‑140 (MQ=255)                    aTAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCAc                                                                                                                    <  2:1093025/140‑1 (MQ=255)                    aTAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCAc                                                                                                                    <  2:918216/140‑1 (MQ=255)                    aTAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCAc                                                                                                                    <  1:789143/140‑1 (MQ=255)                    aTAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCAc                                                                                                                    <  1:604275/140‑1 (MQ=255)                    aTAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCAc                                                                                                                    >  1:1093025/1‑140 (MQ=255)                    aTAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCAc                                                                                                                    <  1:1136732/140‑1 (MQ=255)                      aaTGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGc                                                                                                                       >  2:854937/1‑135 (MQ=255)                      aaTGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGc                                                                                                                       <  1:854937/135‑1 (MQ=255)                      aaTGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACtt                                                                                                                  >  2:745094/1‑140 (MQ=255)                      aaTGCCTAAATCGTTATGCGATGTT‑ACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGc                                                                                                                       <  1:1109354/134‑1 (MQ=255)                      aaTGCCTAAATCGTTATGCGATGTT‑ACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGc                                                                                                                       >  2:1109354/1‑134 (MQ=255)                       aTGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTc                                                                                                                 >  1:337949/1‑140 (MQ=255)                       aTGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTc                                                                                                                 <  2:312244/140‑1 (MQ=255)                       aTGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTc                                                                                                                 >  1:381063/1‑140 (MQ=255)                        tGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGc                                                                                                                       >  1:59976/1‑133 (MQ=255)                        tGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGc                                                                                                                       <  2:59976/133‑1 (MQ=255)                        tGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTcc                                                                                                                >  2:323153/1‑140 (MQ=255)                        tGCCTAAATCGTTATGCGACGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTcc                                                                                                                >  1:947936/1‑140 (MQ=255)                         gCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCt                                                                                                               <  2:381063/140‑1 (MQ=255)                         gCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTCCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCt                                                                                                               <  2:54825/140‑1 (MQ=255)                             aaaTCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAat                                                                                                           >  2:481865/1‑140 (MQ=255)                              aaTCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAata                                                                                                          >  1:808052/1‑140 (MQ=255)                              aaTCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAata                                                                                                          >  2:418039/1‑140 (MQ=255)                               aTCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAatat                                                                                                         <  2:519924/140‑1 (MQ=255)                                   ttATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCg                                                                                                     <  1:481865/140‑1 (MQ=255)                                    tATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCt                                                                                                               <  2:55803/129‑1 (MQ=255)                                    tATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCt                                                                                                               >  1:55803/1‑129 (MQ=255)                                    tATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGa                                                                                                    >  1:433364/1‑140 (MQ=255)                                    tATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGa                                                                                                    >  2:666408/1‑140 (MQ=255)                                     aTGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGAt                                                                                                   <  1:323153/140‑1 (MQ=255)                                     aTGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGAt                                                                                                   <  1:302231/140‑1 (MQ=255)                                     aTGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGAt                                                                                                   <  1:420539/140‑1 (MQ=255)                                     aTGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGAt                                                                                                   <  2:337949/140‑1 (MQ=255)                                     aTGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGAt                                                                                                   <  2:560598/140‑1 (MQ=255)                                        cGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAAc                                                                                                >  2:842777/1‑140 (MQ=255)                                                   aGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATAcc                                                                                     <  2:989282/140‑1 (MQ=255)                                                     acacGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCg                                                                                   >  1:397096/1‑140 (MQ=255)                                                     acacGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCg                                                                                   <  2:765404/140‑1 (MQ=255)                                                      cacGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCTGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGa                                                                                  <  2:545322/140‑1 (MQ=255)                                                      cacGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCTGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGa                                                                                  >  1:545322/1‑140 (MQ=255)                                                       acGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGAc                                                                                 <  1:25069/140‑1 (MQ=255)                                                         gATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGc                                                                               <  1:860787/140‑1 (MQ=255)                                                          ttAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGCGACGAACACTGGGTAGCGTTGTTATCGATATAAGCCCCTTCCTGAATATAGCGATAACTGTAAGATACCCGTCGCa                                                                              <  2:1261469/139‑1 (MQ=255)                                                          aTAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCa                                                                              >  2:594336/1‑140 (MQ=255)                                                          aTAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCa                                                                              <  1:745094/140‑1 (MQ=255)                                                            aaCCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCAtt                                                                            >  1:437107/1‑140 (MQ=255)                                                              ccATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTc                                                                          <  1:418039/140‑1 (MQ=255)                                                               cATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCAttccc                                                                         <  2:437107/140‑4 (MQ=255)                                                               cATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTcc                                                                         <  2:947936/140‑1 (MQ=255)                                                                aTCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCa                                                                        <  2:1050720/140‑1 (MQ=255)                                                                   tttCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATga                                                                     >  1:22218/1‑140 (MQ=255)                                                                   tttCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATga                                                                     >  1:464093/1‑140 (MQ=255)                                                                          aTTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGc                                                              <  1:560554/140‑1 (MQ=255)                                                                           ttttACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCt                                                             <  1:1062413/140‑1 (MQ=255)                                                                           ttttACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCt                                                             <  2:540897/140‑1 (MQ=255)                                                                             ttACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTc                                                           <  2:22218/140‑1 (MQ=255)                                                                                         gggAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAgc                                               >  1:460284/1‑140 (MQ=255)                                                                                               gTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTg                                         >  2:476942/1‑140 (MQ=255)                                                                                                  tCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCa                                      >  1:1257225/1‑140 (MQ=255)                                                                                                   cGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGTCAt                                     <  2:808052/140‑1 (MQ=255)                                                                                                        ggACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCtt                                          <  1:424494/130‑1 (MQ=255)                                                                                                        ggACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCtt                                          >  2:424494/1‑130 (MQ=255)                                                                                                         gACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTcc                               >  1:421622/1‑140 (MQ=255)                                                                                                         gACTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAACCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTcc                               <  1:58709/140‑1 (MQ=255)                                                                                                          aCTTTCATGCTCCAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCa                              >  1:949250/1‑140 (MQ=255)                                                                                                                      cAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAgcg                  <  2:1053963/140‑1 (MQ=255)                                                                                                                      cAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAgcg                  <  2:460284/140‑1 (MQ=255)                                                                                                                      cAGAGACGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAgcg                  >  1:509118/1‑140 (MQ=255)                                                                                                                        gagaCGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCa                >  2:108973/1‑140 (MQ=255)                                                                                                                        gagaCGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCa                >  2:1194643/1‑140 (MQ=255)                                                                                                                        gagaCGAACACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCa                >  2:30523/1‑140 (MQ=255)                                                                                                                              aaCACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTc          <  2:404787/140‑1 (MQ=255)                                                                                                                              aaCACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTc          >  2:868638/1‑140 (MQ=255)                                                                                                                              aaCACTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTc          >  2:224655/1‑140 (MQ=255)                                                                                                                                cacTGGGGAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTccc        >  2:577147/1‑140 (MQ=255)                                                                                                                                     gggAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTtgtg   >  2:112103/1‑140 (MQ=255)                                                                                                                                     gggAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTtgtg   >  1:354317/1‑140 (MQ=255)                                                                                                                                     gggAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTtgtg   >  2:528569/1‑140 (MQ=255)                                                                                                                                      ggAGCGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTgtgt  <  2:949250/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CATGGCGACCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGT  >  NC_000913/478177‑478449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑