Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,201,683	T→C	K2K (AAA→AAG)	zraP ←	zinc responsive, periplasmic protein with chaperone activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,201,683	0	T	C	100.0%	263.7 / NA	75	K2K (AAA→AAG)	zraP	zinc responsive, periplasmic protein with chaperone activity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/41);  total (34/41)

ACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGA  >  NC_000913/4201547‑4201814                                                                                                                                         |                                                                                                                                    ccGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTc                                                                                                                                  <  2:1009630/139‑1 (MQ=255) aCGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTc                                                                                                                                  <  2:1067437/140‑1 (MQ=255) aCGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTc                                                                                                                                  <  1:869661/140‑1 (MQ=255) aCGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTc                                                                                                                                  <  2:487866/140‑1 (MQ=255)    ctgtctgtTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATg                                                                                                                               >  1:140289/1‑140 (MQ=255)      gtctgtTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGt                                                                                                                             <  2:250987/140‑1 (MQ=255)         tgttgtTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTaa                                                                                                                          <  1:70882/140‑1 (MQ=255)         tgttgtTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCACCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTaa                                                                                                                          <  2:707869/140‑1 (MQ=255)             gttCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAAtcct                                                                                                                      >  1:831480/1‑140 (MQ=255)              ttCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAAtcctc                                                                                                                     >  2:462420/1‑140 (MQ=255)               tCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAAtcctcc                                                                                                                    >  1:701420/1‑140 (MQ=255)                cGCTGGTCAAAGGCGCGGCCTTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCa                                                                                                                   >  2:739290/1‑140 (MQ=255)                    ggTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTgg                                                                                                               <  2:142616/140‑1 (MQ=255)                    ggTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTgg                                                                                                               <  1:411191/140‑1 (MQ=255)                     gTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTg                                                                                                                <  1:586100/138‑1 (MQ=255)                     gTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTg                                                                                                                >  2:586100/1‑138 (MQ=255)                     gTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGt                                                                                                              >  1:33571/1‑140 (MQ=255)                              gcgGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACt                                                                                                     <  1:74673/140‑1 (MQ=255)                               cgGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATTGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACtt                                                                                                    <  2:190000/140‑1 (MQ=255)                                ggCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTc                                                                                                   <  2:1030063/140‑1 (MQ=255)                                 gCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCg                                                                                                  <  2:831480/140‑1 (MQ=255)                                  cATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCgg                                                                                                 <  2:95695/140‑1 (MQ=255)                                    ttttGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGt                                                                                               <  2:488301/140‑1 (MQ=255)                                          tgcCACGTACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGGAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCa                                                                                         <  1:739290/140‑1 (MQ=255)                                          tgcCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCa                                                                                         <  1:223641/140‑1 (MQ=255)                                            ccACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCtt                                                                                                        >  1:254497/1‑123 (MQ=255)                                            ccACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCtt                                                                                                        <  2:254497/123‑1 (MQ=255)                                            ccACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATc                                                                                       >  1:521477/1‑140 (MQ=255)                                                      tgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGt                                                                                               <  2:531875/122‑1 (MQ=255)                                                      tgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGt                                                                                               >  1:531875/1‑122 (MQ=255)                                                      tgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCa                                                                                         <  2:1134748/128‑1 (MQ=255)                                                      tgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCa                                                                                         >  1:1134748/1‑128 (MQ=255)                                                      tgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCa                                                                             >  1:437944/1‑140 (MQ=255)                                                      tgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCa                                                                             >  2:1149033/1‑140 (MQ=255)                                                      tgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCa                                                                             >  1:997068/1‑140 (MQ=255)                                                      tgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCa                                                                             >  1:1247263/1‑140 (MQ=255)                                                      tgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTACCa                                                                             >  1:129140/1‑140 (MQ=255)                                                        tcagccgTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAAc                                                                           >  1:502294/4‑140 (MQ=255)                                                        tCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAAc                                                                           >  1:317225/1‑140 (MQ=255)                                                          cgccgTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGa                                                                         >  1:334051/1‑140 (MQ=255)                                                           gccgTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGAt                                                                        <  2:33571/140‑1 (MQ=255)                                                            ccgTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATg                                                                       <  2:701420/140‑1 (MQ=255)                                                            ccgTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATg                                                                       >  1:1011411/1‑140 (MQ=255)                                                              gTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAACTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGaa                                                                     <  2:850623/140‑1 (MQ=255)                                                               tGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGaaa                                                                    <  2:608147/140‑1 (MQ=255)                                                               tGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGaaa                                                                    <  1:462420/140‑1 (MQ=255)                                                                gAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAAc                                                                   >  2:941924/1‑140 (MQ=255)                                                                 aGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACg                                                                  <  2:1247263/140‑1 (MQ=255)                                                                   cAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCt                                                                <  1:495806/140‑1 (MQ=255)                                                                    aaaTGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTg                                                               <  1:252458/140‑1 (MQ=255)                                                                        gCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGatat                                                           <  2:793798/140‑1 (MQ=255)                                                                        gCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGatat                                                           <  1:1051387/140‑1 (MQ=255)                                                                        gCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGatat                                                           <  1:794814/140‑1 (MQ=255)                                                                         cAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTACCAACGATGAAACGCTGATATg                                                          <  2:129140/140‑1 (MQ=255)                                                                         cAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCAGTGCCAACGATGAAACGCTGATATg                                                          <  2:140289/140‑1 (MQ=255)                                                                           gATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGata                                                            <  2:963271/136‑1 (MQ=255)                                                                           gATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGata                                                            >  1:963271/1‑136 (MQ=255)                                                                           gATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGAc                                                        >  1:921761/1‑140 (MQ=255)                                                                                cGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTa                                                   <  2:502294/140‑1 (MQ=255)                                                                                  aTCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGAc                                                        >  2:242478/1‑133 (MQ=255)                                                                                  aTCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGAc                                                        <  1:242478/133‑1 (MQ=255)                                                                                   tCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATc                                                >  2:958482/1‑140 (MQ=255)                                                                                      cATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTgg                                             >  2:333385/1‑140 (MQ=255)                                                                                        tCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCa                                           >  1:859547/1‑140 (MQ=255)                                                                                         cGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCAt                                          >  2:953812/1‑140 (MQ=255)                                                                                               tGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATaaa                                    <  2:437944/140‑1 (MQ=255)                                                                                                    aaaGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGt                               <  1:787175/140‑1 (MQ=255)                                                                                                         gcCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTaaaaa                          >  1:644741/1‑140 (MQ=255)                                                                                                         gcCATCATTACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTaaaaa                          >  1:1240124/1‑140 (MQ=255)                                                                                                                 tACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGc                  <  2:859547/140‑1 (MQ=255)                                                                                                                 tACCAGGGCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGc                  <  2:356810/140‑1 (MQ=255)                                                                                                                       ggCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCctgctg            >  2:794882/1‑140 (MQ=255)                                                                                                                       ggCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCctgctg            >  1:1238953/1‑140 (MQ=255)                                                                                                                       ggCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCctgctg            >  2:372963/1‑140 (MQ=255)                                                                                                                        gCAATTTTCGTGTTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCtgctgc           >  1:18688/1‑140 (MQ=255)                                                                                                                                 gtgtTCCGCTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGa  >  2:12819/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    ACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGA  >  NC_000913/4201547‑4201814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑