Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,495,235	G→A	R325R (CGC→CGT)	ahr ←	aldehyde reductase, NADPH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,495,235	0	G	A	100.0%	188.5 / NA	54	R325R (CGC→CGT)	ahr	aldehyde reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/24);  total (30/24)

CGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACG  >  NC_000913/4495099‑4495367                                                                                                                                         |                                                                                                                                     cGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCacgc                                                                                                                                   <  1:888414/140‑1 (MQ=255) cGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCacgc                                                                                                                                   >  1:737749/1‑140 (MQ=255)   aaCAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGcac                                                                                                                                 >  2:963419/1‑140 (MQ=255)        tGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCt                                                                                                                            >  1:733522/1‑140 (MQ=255)                ggCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGcg                                                                                                                    >  2:298984/1‑140 (MQ=255)                  cTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCacgc                                                                                                                                   >  1:342257/1‑123 (MQ=255)                  cTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCacgc                                                                                                                                   <  2:342257/123‑1 (MQ=255)                  cTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGcgtc                                                                                                                  >  1:1261727/1‑140 (MQ=255)                    gtgCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGcgtcgt                                                                                                                <  2:733522/140‑1 (MQ=255)                    gtgCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGcgtcgt                                                                                                                >  1:499949/1‑140 (MQ=255)                    gtgCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGcgtcgt                                                                                                                >  1:784207/1‑140 (MQ=255)                      gCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTa                                                                                                              >  1:293010/1‑140 (MQ=255)                           cGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAAtttt                                                                                                         <  1:804452/140‑1 (MQ=255)                             aTACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCg                                                                                                       <  2:187166/140‑1 (MQ=255)                             aTACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCg                                                                                                       <  2:737749/140‑1 (MQ=255)                                  aCAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATc                                                                                                  <  2:551367/140‑1 (MQ=255)                                  aCAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATc                                                                                                  <  2:1062533/140‑1 (MQ=255)                                         aGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATAGCGTCGTTAATTTTCGACATCGGGAACa                                                                                           <  2:1052250/140‑1 (MQ=255)                                                 tgCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCggtg                                                                                   >  2:1134606/1‑140 (MQ=255)                                                  gCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCggtgg                                                                                  <  2:238929/140‑1 (MQ=255)                                                  gCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCggtgg                                                                                  <  1:517309/140‑1 (MQ=255)                                                  gCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCggtgg                                                                                  >  1:796321/1‑140 (MQ=255)                                                  gCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCggtgg                                                                                  >  2:517309/1‑140 (MQ=255)                                                   cGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCggtggt                                                                                 >  2:491032/1‑140 (MQ=255)                                                     aaaGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATTGGGAACAGTTCGGTGGTCg                                                                               >  1:982186/1‑140 (MQ=255)                                                     aaaGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCg                                                                               >  1:1019001/1‑140 (MQ=255)                                                     aaaGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCg                                                                               >  2:514615/1‑140 (MQ=255)                                                      aaGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCgg                                                                              <  2:323698/140‑1 (MQ=255)                                                        gagGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGgcg                                                                            <  1:828310/140‑1 (MQ=255)                                                             cAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAAcc                                                                       <  2:1166078/140‑1 (MQ=255)                                                                cgcCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTtg                                                                    >  1:1145578/1‑140 (MQ=255)                                                                 acagcAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCCACCTtgc                                                                   >  1:1104887/5‑140 (MQ=255)                                                                  ccTCAGATCAGCGCTGCGGATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTtgct                                                                  >  2:223355/1‑140 (MQ=255)                                                                          cAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTgcgggcgg                                                          <  2:790574/140‑1 (MQ=255)                                                                             cgcTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCaa                                                       >  1:975520/1‑140 (MQ=255)                                                                             cgcTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCaa                                                       >  1:607151/1‑140 (MQ=255)                                                                             cgcTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCaa                                                       <  1:514615/140‑1 (MQ=255)                                                                             cgcTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCaa                                                       >  1:347071/1‑140 (MQ=255)                                                                                  cGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTTACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGc                                                  >  2:70337/1‑140 (MQ=255)                                                                                  cGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAAcac                                                  >  2:10299/1‑138 (MQ=255)                                                                                   gAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCa                                                 <  1:491032/140‑1 (MQ=255)                                                                                   gAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCa                                                 <  1:11871/140‑1 (MQ=255)                                                                                   gAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCa                                                 <  1:298984/140‑1 (MQ=255)                                                                                           ttCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTa                                         <  2:784207/140‑1 (MQ=255)                                                                                             cAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACg                                       <  1:1134606/140‑1 (MQ=255)                                                                                             cAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACg                                       <  1:211211/140‑1 (MQ=255)                                                                                              aaaaaTCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGc                                      <  2:1145578/140‑1 (MQ=255)                                                                                              aaaaaTCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACACATCAGCTTACGc                                      <  1:10299/140‑1 (MQ=255)                                                                                                                  acGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGt                  >  2:693764/1‑140 (MQ=255)                                                                                                                  acGCGGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGt                  >  1:1120052/1‑140 (MQ=255)                                                                                                                   cgcgGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTa                 >  2:601486/1‑140 (MQ=255)                                                                                                                   cgcgGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTa                 >  2:491577/1‑140 (MQ=255)                                                                                                                   cgcgGTAACGCGCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTa                 >  1:409972/1‑140 (MQ=255)                                                                                                                           cgcgCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAAc          <  2:646715/139‑1 (MQ=255)                                                                                                                           cgcgCCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAAc          >  1:646715/1‑139 (MQ=255)                                                                                                                             cgcCTTACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCag       <  2:1261727/140‑1 (MQ=255)                                                                                                                                  tACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACg  >  1:818773/1‑140 (MQ=255)                                                                                                                                  tACCGTCACGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCAGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACg  >  1:315699/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     CGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCGGCGCAACCTTGCTGCGGGCGGCAAAACGCATCAGCTTACGCAGCTCATAAGGCGTGCCGGTAGCAGAACCAGAGACG  >  NC_000913/4495099‑4495367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑