Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,532,980	A→G	S68S (AGT→AGC)	yjhP ←	KpLE2 phage‑like element; putative methyltransferase YjhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,532,980	0	A	G	100.0%	126.1 / NA	37	S68S (AGT→AGC)	yjhP	KpLE2 phage‑like element; putative methyltransferase YjhP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/16);  total (21/16)

ATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTT  >  NC_000913/4532844‑4533114                                                                                                                                         |                                                                                                                                       aTGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTc                                                                                                                                     <  1:895528/140‑1 (MQ=255)       cgcCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTTCCTGCGCGGTGAAGAGCGAGCTCATGTCg                                                                                                                               >  2:401620/1‑140 (MQ=255)                    cgccACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCg                                                                                                                  <  2:633107/140‑1 (MQ=255)                            cacaTTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCa                                                                                                          >  2:381339/1‑140 (MQ=255)                             acaTTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCAt                                                                                                         <  2:1031062/140‑1 (MQ=255)                                  tttCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATcc                                                                                                    <  2:1213194/140‑1 (MQ=255)                                   ttCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCAt                                                                                                         <  1:244745/134‑1 (MQ=255)                                   ttCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCAt                                                                                                         >  2:244745/1‑134 (MQ=255)                                   ttCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATccc                                                                                                   <  2:907124/140‑1 (MQ=255)                                   ttCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATccc                                                                                                   <  2:1134881/140‑1 (MQ=255)                                    tCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGTCGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCt                                                                                                  <  2:1106085/140‑1 (MQ=255)                                        ttGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGcc                                                                                              <  1:738351/140‑1 (MQ=255)                                         tGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGccc                                                                                             >  2:214418/1‑140 (MQ=255)                                               gTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGc                                                                                       >  2:84782/1‑140 (MQ=255)                                                                        aaaTGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGcc                                                              <  1:214418/140‑1 (MQ=255)                                                                        aaaTGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGcc                                                              <  1:326542/140‑1 (MQ=255)                                                                              aCGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTc                                                        <  1:75465/140‑1 (MQ=255)                                                                              aCGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTc                                                        >  2:1056194/1‑140 (MQ=255)                                                                               cgcgTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGcc                                                              >  1:216396/1‑133 (MQ=255)                                                                               cgcgTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGcc                                                              <  2:216396/133‑1 (MQ=255)                                                                               cgcgTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCa                                                       >  2:597242/1‑140 (MQ=255)                                                                               cgcgTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCa                                                       >  2:554407/1‑140 (MQ=255)                                                                                  gTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGa                                                    >  1:14994/1‑140 (MQ=255)                                                                                   ttCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGaa                                                   >  2:992925/1‑140 (MQ=255)                                                                                               gAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCg                                       <  1:84782/140‑1 (MQ=255)                                                                                                      tcCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCAt                                >  2:208689/1‑140 (MQ=255)                                                                                                          cgcgACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATgcgc                            >  2:707913/1‑140 (MQ=255)                                                                                                            cgACGCTTTGCCTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCgcag                          >  2:990475/1‑140 (MQ=255)                                                                                                                       cTGCGCGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCa               >  2:961071/1‑140 (MQ=255)                                                                                                                         gcgcGGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGc             >  1:77179/1‑140 (MQ=255)                                                                                                                          cgcgGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCg            >  1:992118/1‑140 (MQ=255)                                                                                                                          cgcgGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCg            <  1:990475/140‑1 (MQ=255)                                                                                                                           gcgGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGt           >  2:462117/1‑140 (MQ=255)                                                                                                                            cgGTGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTg          <  2:77179/140‑1 (MQ=255)                                                                                                                               tGAAGAGCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCg       >  2:1085917/1‑140 (MQ=255)                                                                                                                                    agCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACtt  >  2:149058/1‑140 (MQ=255)                                                                                                                                    agCGAGCTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACtt  >  2:962470/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTT  >  NC_000913/4532844‑4533114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑