Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,992	C→T	L177L (CTG→CTA)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,992	0	C	T	100.0%	157.0 / NA	46	L177L (CTG→CTA)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/21);  total (25/21)

GTAACGGTCGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAA  >  NC_000913/4629863‑4630128                                                                                                                                  |                                                                                                                                         gTAACGGTCGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAggcggc                                                                                                                                >  1:644182/1‑140 (MQ=255)  tAACGGTCGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGca                                                                                                                               <  2:881102/140‑1 (MQ=255)       gTCGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCATCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACg                                                                                                                          <  2:781220/140‑1 (MQ=255)       gTCGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACg                                                                                                                          >  1:781220/1‑140 (MQ=255)         cGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCt                                                                                                                        >  1:826258/1‑140 (MQ=255)            gggTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTAcg                                                                                                                     >  2:669435/1‑140 (MQ=255)                  tCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGcgacga                                                                                                               >  2:419799/1‑140 (MQ=255)                  tCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGcgacga                                                                                                               >  1:827826/1‑140 (MQ=255)                         aaCGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTcac                                                                                                        >  2:246222/1‑140 (MQ=255)                           cGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTcacca                                                                                                      <  2:736872/140‑1 (MQ=255)                           cGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTcacca                                                                                                      <  1:246222/140‑1 (MQ=255)                                ccTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGga                                                                                                 <  1:649197/140‑1 (MQ=255)                                        gTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAg                                                                                         >  1:950046/1‑140 (MQ=255)                                                   aGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTcgcg                                                                              >  2:165960/1‑140 (MQ=255)                                                      gTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTT‑‑‑CACCGGAGAGGTTAGCGATTTTCGCGTCCCAg                                                                        >  2:488463/1‑140 (MQ=255)                                                       ttCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTccc                                                                          >  1:495804/1‑140 (MQ=255)                                                            gccagGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTcc                                                                     >  1:958552/1‑140 (MQ=255)                                                             ccagGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCg                                                                    >  1:656000/1‑140 (MQ=255)                                                              cagGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTCTCGCGTCCCAGTCCgg                                                                   <  2:950046/140‑1 (MQ=255)                                                                                  cAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATccgccg                                               >  2:337556/1‑140 (MQ=255)                                                                                  cAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATccgccg                                               <  1:891112/140‑1 (MQ=255)                                                                                  cAGGTGGTGGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATccgccg                                               <  1:194088/140‑1 (MQ=255)                                                                                    ggtggtTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCa                                             <  1:795581/140‑1 (MQ=255)                                                                                    ggtggtTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCa                                             <  2:644182/140‑1 (MQ=255)                                                                                       ggttggtCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGc                                          <  2:656000/140‑1 (MQ=255)                                                                                        gttggtCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCt                                         >  2:666138/1‑140 (MQ=255)                                                                                            gtCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAg                                     >  2:1219592/1‑140 (MQ=255)                                                                                                gTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCa                                             <  1:381000/128‑1 (MQ=255)                                                                                                gTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCa                                             >  2:381000/1‑128 (MQ=255)                                                                                                   cgtcgAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCa                                             >  2:1192672/1‑125 (MQ=255)                                                                                                   cgtcgAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCa                                             <  1:1192672/125‑1 (MQ=255)                                                                                                     tcgAGCAGCAGCATGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGtt                            <  1:666138/140‑1 (MQ=255)                                                                                                            gcagcaTGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTa                     <  2:827826/140‑1 (MQ=255)                                                                                                                caTGTCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGAc                 <  2:958552/140‑1 (MQ=255)                                                                                                                    tCTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACcgtc             >  1:312064/1‑140 (MQ=255)                                                                                                                     cTGGTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCTATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGAACGTTCAGATTATGACcgtcg            >  2:859304/1‑140 (MQ=255)                                                                                                                       ggTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGAc                 <  1:721225/133‑1 (MQ=255)                                                                                                                       ggTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGAc                 >  2:721225/1‑133 (MQ=255)                                                                                                                       ggTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACcgtc             >  2:988841/1‑137 (MQ=255)                                                                                                                       ggTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACcgtc             <  1:988841/137‑1 (MQ=255)                                                                                                                       ggTTTTTCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTg          <  2:826258/140‑1 (MQ=255)                                                                                                                         tttttCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAg        <  1:1219592/140‑1 (MQ=255)                                                                                                                           tttCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGcc      >  1:352504/1‑140 (MQ=255)                                                                                                                             tCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACcgtc             <  2:684038/131‑1 (MQ=255)                                                                                                                             tCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACcgtc             >  1:684038/1‑131 (MQ=255)                                                                                                                             tCCAGTAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGGTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTg    >  2:888909/1‑140 (MQ=255)                                                                                                                               cagtagcagGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGaa  <  2:495804/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                         GTAACGGTCGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAA  >  NC_000913/4629863‑4630128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑