Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,631,420	A→G	T230A (ACC→GCC)	slt →	soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,631,420	0	A	G	100.0%	229.5 / NA	65	T230A (ACC→GCC)	slt	soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/34);  total (31/34)

AGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGA  >  NC_000913/4631289‑4631556                                                                                                                                    |                                                                                                                                            agCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTcgcg                                                                                                                                     >  1:322169/1‑140 (MQ=255)   cGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTcgcgcg                                                                                                                                   <  2:1240148/140‑1 (MQ=255)   cGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTcgcgcg                                                                                                                                   <  2:711902/140‑1 (MQ=255)    gTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGt                                                                                                                                  >  1:598268/1‑140 (MQ=255)      aTCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑c                                                                                                                             >  1:888861/1‑140 (MQ=255)       tCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑Ca                                                                                                                            >  2:311942/1‑140 (MQ=255)                 gATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGa                                                                                                                  <  2:888861/140‑1 (MQ=255)                  aTGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGAc                                                                                                                 >  1:429290/1‑140 (MQ=255)                     aaaGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGa                                                                                                              <  2:598268/140‑1 (MQ=255)                           ggTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTAc                                                                                                        <  2:322169/140‑1 (MQ=255)                             tAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTAccc                                                                                                      >  1:727143/1‑140 (MQ=255)                                     gCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATg                                                                                              >  1:1177946/1‑140 (MQ=255)                                     gCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATg                                                                                              >  2:269970/1‑140 (MQ=255)                                      ccTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATgg                                                                                             <  2:244509/140‑1 (MQ=255)                                           tAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCA‑TTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGcggcgg                                                                                       <  2:947870/140‑1 (MQ=255)                                              cAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTg                                                                                     <  2:608105/140‑1 (MQ=255)                                               aGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTgg                                                                                    >  2:948752/1‑140 (MQ=255)                                               aGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTgg                                                                                    >  2:256978/1‑140 (MQ=255)                                                 tGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCg                                                                                  <  2:429290/140‑1 (MQ=255)                                                 tGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCg                                                                                  >  2:416828/1‑140 (MQ=255)                                                    tGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACCGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGttt                                                                               >  2:1110958/1‑140 (MQ=255)                                                     ggcagggcagATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTg                                                                              >  2:94059/1‑140 (MQ=255)                                                        agggcagATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCa                                                                           <  1:1110958/140‑1 (MQ=255)                                                                tgcctgccGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGgcg                                                                   >  2:729706/1‑140 (MQ=255)                                                                tgcctgccGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGgcg                                                                   <  2:978991/140‑1 (MQ=255)                                                                    tgccGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTACAGCAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGgcgc                                                                  >  1:270206/1‑140 (MQ=255)                                                                    tgccGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGc                                                               >  2:929052/1‑140 (MQ=255)                                                                     gccGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCa                                                              <  1:1218459/140‑1 (MQ=255)                                                                     gccGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCa                                                              <  2:739392/140‑1 (MQ=255)                                                                     gccGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCa                                                              <  1:311942/140‑1 (MQ=255)                                                                      ccGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAg                                                             <  1:948752/140‑1 (MQ=255)                                                                       cGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAgg                                                            <  1:416828/140‑1 (MQ=255)                                                                              cAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTga                                                     <  2:727143/140‑1 (MQ=255)                                                                                 aCTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGaa                                                  >  1:649352/1‑140 (MQ=255)                                                                                   tATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATg                                                         >  1:935554/1‑131 (MQ=255)                                                                                   tATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATg                                                         <  2:935554/131‑1 (MQ=255)                                                                                   tATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATg                                                <  1:971446/140‑1 (MQ=255)                                                                                     tCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCa                                              >  1:965019/1‑140 (MQ=255)                                                                                     tCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCa                                              >  1:892019/1‑140 (MQ=255)                                                                                     tCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCa                                              >  2:903836/1‑140 (MQ=255)                                                                                      cGCCTCGGCAATCATTTCACTGGCGAACAAGCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCAc                                             <  2:879086/140‑1 (MQ=255)                                                                                      cGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCAc                                             <  2:237619/140‑1 (MQ=255)                                                                                      cGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCAc                                             <  1:256978/140‑1 (MQ=255)                                                                                       gCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACg                                            <  1:1171684/140‑1 (MQ=255)                                                                                             gCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCtgat                                      >  1:1129099/1‑140 (MQ=255)                                                                                             gCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCtgat                                      <  1:382802/140‑1 (MQ=255)                                                                                             gCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCtgat                                      >  2:498566/1‑140 (MQ=255)                                                                                                            gCGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGc                       >  1:647727/1‑140 (MQ=255)                                                                                                             cGAACAACCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGcc                      >  2:161389/1‑140 (MQ=255)                                                                                                               aacaacCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCa                    >  2:204222/1‑140 (MQ=255)                                                                                                                   acCCTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGgcg                <  1:498566/140‑1 (MQ=255)                                                                                                                     ccTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCgca              <  2:1177946/140‑1 (MQ=255)                                                                                                                     ccTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCgca              <  1:161389/140‑1 (MQ=255)                                                                                                                      cTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCgcag             <  2:1191345/140‑1 (MQ=255)                                                                                                                      cTAATACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCgcag             <  2:965019/140‑1 (MQ=255)                                                                                                                      cTAATACGGTACTGGCATTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCgcag             <  2:1129099/140‑1 (MQ=255)                                                                                                                         aTACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGc                       <  2:865898/127‑1 (MQ=255)                                                                                                                         aTACGGTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGc                       >  1:865898/1‑127 (MQ=255)                                                                                                                              gTACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCCGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTaa     >  1:855715/1‑140 (MQ=255)                                                                                                                               tACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCgcag             <  1:938437/131‑1 (MQ=255)                                                                                                                               tACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCgcag             >  2:938437/1‑131 (MQ=255)                                                                                                                               tACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAAt    >  2:403109/1‑140 (MQ=255)                                                                                                                               tACTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAAt    >  1:837841/1‑140 (MQ=255)                                                                                                                               gaCTGGCCTTCGCGTGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAAt    <  1:318116/139‑1 (MQ=255)                                                                                                                                 cTGGCCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGa  <  1:1017897/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                            AGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTA‑‑‑CAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGA  >  NC_000913/4631289‑4631556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑