Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	511,054	C→T	S109N (AGC→AAC)	copA ←	Cu(+) exporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	511,054	0	C	T	100.0%	209.8 / NA	59	S109N (AGC→AAC)	copA	Cu(+) exporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/29);  total (30/29)

CCGCCTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTGGGTGGCTTACA  >  NC_000913/510920‑511188                                                                                                                                       |                                                                                                                                       ccGCCTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATg                                                                                                                                   <  2:975295/140‑1 (MQ=255) ccGCCTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATg                                                                                                                                   <  1:740270/140‑1 (MQ=255)   gCCTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGcc                                                                                                                                 >  2:31278/1‑140 (MQ=255)   gCCTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGcc                                                                                                                                 >  2:59784/1‑140 (MQ=255)                    tgGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAActgct                                                                                                                <  2:888900/140‑1 (MQ=255)                           gCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTgtc                                                                                                         >  2:575753/1‑140 (MQ=255)                           gCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTgtc                                                                                                         >  1:359894/1‑140 (MQ=255)                                    aTCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTgtcatcgtcatc                                                                                                >  2:911097/1‑140 (MQ=255)                                     tCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGtcatcgtcatcg                                                                                               <  1:326475/140‑1 (MQ=255)                                           gcgcAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATcggcggcc                                                                                         >  2:272415/1‑138 (MQ=255)                                           gcgcAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTc                                                                                         >  2:722255/1‑140 (MQ=255)                                           gcgcAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTc                                                                                         <  2:879596/140‑1 (MQ=255)                                           gcgcAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTc                                                                                         >  2:504923/1‑140 (MQ=255)                                           gcgcAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTc                                                                                         >  1:879596/1‑140 (MQ=255)                                            cgcAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCg                                                                                        >  2:373887/1‑140 (MQ=255)                                            cgcAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCg                                                                                        >  2:22402/1‑140 (MQ=255)                                                gTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGc                                                                                    >  2:277751/1‑140 (MQ=255)                                                    gCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGa                                                                                <  2:977735/140‑1 (MQ=255)                                                      tCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAg                                                                              >  2:336442/1‑140 (MQ=255)                                                       ccGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAgc                                                                             <  2:1169528/140‑1 (MQ=255)                                                       ccGCCAGGTTTACCCGTGCCTGAGGCAGGCCTGGGACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCGGTGCACGTCGCTCAGCAGCAACTGCTGGCTGTCAGCGTCGTCGGCGGTCGCTGCCGGAAgc                                                                             <  2:283387/140‑1 (MQ=255)                                                                ttACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGaa                                                                    <  1:722255/140‑1 (MQ=255)                                                                  aCCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAAc                                                                  <  1:1215626/140‑1 (MQ=255)                                                                  aCCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGCGGCGGACCGAAGGGCCTCCGCAAc                                                                  >  2:263041/1‑140 (MQ=255)                                                                   cccGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAAcc                                                                 <  2:359894/140‑1 (MQ=255)                                                                   cccGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAAcc                                                                 >  2:1015417/1‑140 (MQ=255)                                                                        gCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGGAGCGCCCCCGAAACCGCTGt                                                            >  2:881126/1‑140 (MQ=255)                                                                        gCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAgcg                                                                            <  1:631170/124‑1 (MQ=255)                                                                        gCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAgcg                                                                            >  2:631170/1‑124 (MQ=255)                                                                        gCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCCGAAACCCCTGt                                                            >  1:1030281/1‑140 (MQ=255)                                                                        gCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGt                                                            >  1:725450/1‑140 (MQ=255)                                                                         ccTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTc                                                           >  1:784579/1‑140 (MQ=255)                                                                         ccTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTc                                                           >  1:556361/1‑140 (MQ=255)                                                                             aGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTg                                                       <  1:22402/140‑1 (MQ=255)                                                                                gACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCCGGAGCGCCCCAGAAACCGCCGTCAGAGCtt                                                    >  2:855020/1‑140 (MQ=255)                                                                                    cccGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGa                                                <  2:784579/140‑1 (MQ=255)                                                                                    cccGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGa                                                <  1:336442/140‑1 (MQ=255)                                                                                      cGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACg                                              <  1:575753/140‑1 (MQ=255)                                                                                                tGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGGAGCGCCCCAGAAAACGCCGTCTGTGCTTCCGCCGGGGTtgttga                                    >  2:1155752/1‑140 (MQ=255)                                                                                                   aGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCCGGAGCGCCTCAGAAAACGCTGTCAGTGCTTCCCACGGGGTTGATGACTc                                 >  2:573831/1‑140 (MQ=255)                                                                                                       cATTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGGAGCGCCTCAGGAACCGCTGGCAGGGGTTCCGCCGGGGGTTATGACTCcccc                             >  1:705589/1‑140 (MQ=255)                                                                                                        aTTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCa                            <  1:956656/140‑1 (MQ=255)                                                                                                        aTTTTGTACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCa                            <  1:228246/140‑1 (MQ=255)                                                                                                              tACGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGtt                      <  1:573831/140‑1 (MQ=255)                                                                                                               aCGCGGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGttt                     <  1:881126/140‑1 (MQ=255)                                                                                                                  cgGGTGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGc                  <  2:556361/140‑1 (MQ=255)                                                                                                                      tGACACAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCCCAGAAACCGCCGTCAGTGCTTCCGACGGGGTTGATGACTCCCCCCGCGGGTTAGCCctt              >  2:760445/1‑140 (MQ=255)                                                                                                                        acacAGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTgg            <  1:68427/140‑1 (MQ=255)                                                                                                                         cacaGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTggg           <  1:59784/140‑1 (MQ=255)                                                                                                                         cacaGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTggg           <  1:855020/140‑1 (MQ=255)                                                                                                                         cacaGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTggg           <  1:121547/140‑1 (MQ=255)                                                                                                                         cacaGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTggg           <  1:1155752/140‑1 (MQ=255)                                                                                                                         cacaGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTggg           <  1:1137673/140‑1 (MQ=255)                                                                                                                         cacaGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTggg           <  1:1015417/140‑1 (MQ=255)                                                                                                                           caGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTGGGTg         <  1:760445/140‑1 (MQ=255)                                                                                                                           caGCTGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTGGGTg         <  1:929843/140‑1 (MQ=255)                                                                                                                               tGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTGGGTGGCtt     >  1:30744/1‑140 (MQ=255)                                                                                                                               tGGCGCAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAAACGCTGTCAGTGCTTCCCACGGGGTTGATGACTCCGCCCGCGGGTTTGCCTTTTGGGGGGtt     >  2:929843/1‑140 (MQ=255)                                                                                                                                 gcgcAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGGTTGATGACTCCCCCCGCGGGTTAGCCTTTTGGGGGCtttc   >  2:68427/1‑138 (MQ=255)                                                                                                                                  cgcAGTTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCCCCGCCGGGAGCGCCTCAGAAAACGCTGTCAGTGCTTCCCCCGGGGTTTATGAATCCGCCAGCGGGTTTGCCTTTTGGTGGCTTACa  >  2:493384/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       CCGCCTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTGGGTGGCTTACA  >  NC_000913/510920‑511188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑