Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	577,732	A→G	I40M (ATA→ATG)	rrrD →	DLP12 prophage; lysozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	577,732	0	A	G	100.0%	176.4 / NA	50	I40M (ATA→ATG)	rrrD	DLP12 prophage; lysozyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/27);  total (23/27)

GGCTGCGAGAGGTGAATAATGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATACCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAATGCAAAGCACTCTTGAATAAAGACCTTGCCACTGTCGCC  >  NC_000913/577595‑577861                                                                                                                                          |                                                                                                                                  ggCTGCGAGAGGTGAATAATGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTAcatgcc                                                                                                                                 >  1:309129/1‑140 (MQ=255) ggCTGCGAGAGGTGAATAATGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTAcatgcc                                                                                                                                 >  2:4985/1‑140 (MQ=255)  gCTGCGAGAGGTGAATAATGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTAcatgcca                                                                                                                                <  2:611036/140‑1 (MQ=255)      cGAGAGGTGAATAATGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACa                                                                                                                            >  1:99232/1‑140 (MQ=255)      cGAGAGGTGAATAATGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACa                                                                                                                            >  1:528811/1‑140 (MQ=255)          agGTGAATAATGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGa                                                                                                                        <  2:99232/140‑1 (MQ=255)          agGTGAATAATGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGa                                                                                                                        <  2:33343/140‑1 (MQ=255)               aataatGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATAttg                                                                                                                   <  2:1038906/140‑1 (MQ=255)               aataatGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATAttg                                                                                                                   <  2:322845/140‑1 (MQ=255)               aataatGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATAttg                                                                                                                   <  1:865684/140‑1 (MQ=255)                ataatGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATAttgt                                                                                                                  >  2:121111/1‑140 (MQ=255)                 taatGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATAttgtt                                                                                                                 <  2:571577/140‑1 (MQ=255)                 taatGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATAttgtt                                                                                                                 <  1:892074/140‑1 (MQ=255)                      ccTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGgtgt                                                                                                            >  2:10414/1‑140 (MQ=255)                       cTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGTGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTa                                                                                                           <  2:353969/140‑1 (MQ=255)                       cTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTa                                                                                                           >  2:540787/1‑140 (MQ=255)                         ccATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATAttgtt                                                                                                                 >  2:188231/1‑132 (MQ=255)                         ccATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATAttgtt                                                                                                                 <  1:188231/132‑1 (MQ=255)                         ccATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATg                                                                                                         >  2:353810/1‑140 (MQ=255)                           atcatTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGa                                                                                                       <  1:539555/140‑1 (MQ=255)                           atcatTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGa                                                                                                       <  1:121111/140‑1 (MQ=255)                               ttACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGt                                                                                                   <  2:197480/140‑1 (MQ=255)                                tACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTa                                                                                                  >  1:308074/1‑140 (MQ=255)                                    aaaaGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTc                                                                                              <  1:540787/140‑1 (MQ=255)                                         ccGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACg                                                                                           <  1:649000/138‑1 (MQ=255)                                         ccGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACg                                                                                           >  2:649000/1‑138 (MQ=255)                                             tgctgctgcTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGacaca                                                                                     <  1:180480/140‑1 (MQ=255)                                                   tgctATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGaa                                                                               >  2:358135/1‑140 (MQ=255)                                                            tggCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGAcatca                                                                      >  1:60633/1‑140 (MQ=255)                                                             ggCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGAcatcat                                                                     >  1:1125480/1‑140 (MQ=255)                                                              gCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATg                                                                    >  2:359271/1‑140 (MQ=255)                                                               cGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGc                                                                   <  1:898530/140‑1 (MQ=255)                                                                ggAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTCGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCt                                                                  <  2:1175726/140‑1 (MQ=255)                                                                            ataGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGta                                                      <  2:1030599/140‑1 (MQ=255)                                                                            ataGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGta                                                      <  2:60633/140‑1 (MQ=255)                                                                            ataGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGta                                                      <  1:4985/140‑1 (MQ=255)                                                                               gCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATAc                                                   >  1:923053/1‑140 (MQ=255)                                                                                 aTCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCa                                                 <  1:10414/140‑1 (MQ=255)                                                                                    aGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAg                                              >  1:247536/1‑140 (MQ=255)                                                                                     gtgtTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGc                                             >  2:1157450/1‑140 (MQ=255)                                                                                          aaTCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAAt                                        >  1:725219/1‑140 (MQ=255)                                                                                          aaTCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAAt                                        >  1:926622/1‑140 (MQ=255)                                                                                                tGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAATGCAAAg                                  >  2:412662/1‑140 (MQ=255)                                                                                                  gCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAATGCAAAg                                  <  2:319884/138‑1 (MQ=255)                                                                                                  gCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAATGCAAAg                                  >  1:319884/1‑138 (MQ=255)                                                                                                  gCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAATGCAAAGCa                                <  1:412662/140‑1 (MQ=255)                                                                                                  gCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAATGCAAAGCa                                <  1:359271/140‑1 (MQ=255)                                                                                                      aaGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAATGCAAAGCActct                            <  1:358135/140‑1 (MQ=255)                                                                                                       aGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAATGCAAAGCa                                >  1:182132/1‑135 (MQ=255)                                                                                                       aGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAATGCAAAGCa                                <  2:182132/135‑1 (MQ=255)                                                                                                       aGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAATGCAAAGCACTCtt                           <  2:247536/140‑1 (MQ=255)                                                                                                                   ggTCTGGAAGGTGTCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAATGCAAAGCACTCTTGAATAAAGACCt               >  2:506415/1‑140 (MQ=255)                                                                                                                                tCAGCTACATGCCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAATGCAAAGCACTCTTGAATAAAGACCTTGCCACTGTCGcc  >  2:42150/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                  GGCTGCGAGAGGTGAATAATGCCTCCATCATTACGAAAAGCCGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGGCCCAAGTGGTAACGATGGTCTGGAAGGTGTCAGCTACATACCATACAAAGATATTGTTGGTGTATGGACTGTATGTCACGGACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCAAAGCAGAATGCAAAGCACTCTTGAATAAAGACCTTGCCACTGTCGCC  >  NC_000913/577595‑577861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑