Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	587,227	C→T	G252D (GGC→GAC)	ybcH ←	DUF4434 domain‑containing protein YbcH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	587,227	0	C	T	100.0%	300.8 / NA	85	G252D (GGC→GAC)	ybcH	DUF4434 domain‑containing protein YbcH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/43);  total (42/43)

TCGAGAATGCCGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGCCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCACGCCGCTGCCATCCTGTACCCA  >  NC_000913/587098‑587360                                                                                                                                  |                                                                                                                                      tCGAGAATGCCGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGc                                                                                                                             >  1:1148920/1‑140 (MQ=255) tCGAGAATGCCGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGc                                                                                                                             >  1:181022/1‑140 (MQ=255)  cGAGAATGCCGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCg                                                                                                                            >  1:403681/1‑140 (MQ=255)  cGAGAATGCCGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCg                                                                                                                            >  1:155863/1‑140 (MQ=255)  agagaATGCCGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCg                                                                                                                            >  1:957307/2‑140 (MQ=255)   gagaATGCCGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGa                                                                                                                           >  2:1186170/1‑140 (MQ=255)   gagaATGCCGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGa                                                                                                                           >  1:737847/1‑140 (MQ=255)        tGCCGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACaaaa                                                                                                                      <  2:403681/140‑1 (MQ=255)        tGCCGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACaaaa                                                                                                                      >  2:329888/1‑140 (MQ=255)        tGCCGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACaaaa                                                                                                                      >  2:170472/1‑140 (MQ=255)           cGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGt                                                                                                                   >  2:852214/1‑140 (MQ=255)           cGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGt                                                                                                                   >  2:422138/1‑140 (MQ=255)           cGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGt                                                                                                                   >  1:531090/1‑140 (MQ=255)             tGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTc                                                                                                                 >  1:479644/1‑140 (MQ=255)             tGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTc                                                                                                                 >  2:850512/1‑140 (MQ=255)               cgcgACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCAt                                                                                                               >  1:690353/1‑140 (MQ=255)                       gCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAAcg                                                                                                       <  2:232481/140‑1 (MQ=255)                           aTAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAAcgccgc                                                                                                   <  2:1216203/140‑1 (MQ=255)                               cgcAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGc                                                                                               <  1:1186170/140‑1 (MQ=255)                               cgcAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGc                                                                                               <  1:760070/140‑1 (MQ=255)                                 cAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAg                                                                                             >  2:438971/1‑140 (MQ=255)                                      agaAATAGAGAGTGTCTTTACTGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCg                                                                                        >  1:457634/1‑140 (MQ=255)                                      agaAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCg                                                                                        >  2:1146665/1‑140 (MQ=255)                                              agagTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTg                                                                                >  1:516869/1‑140 (MQ=255)                                              agagTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTg                                                                                <  1:74872/140‑1 (MQ=255)                                              agagTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTg                                                                                >  2:916103/1‑140 (MQ=255)                                                agTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGc                                                                              >  2:1179038/1‑140 (MQ=255)                                                agTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGc                                                                              >  1:609016/1‑140 (MQ=255)                                                agTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCAGGACTTTGGc                                                                              >  1:1174433/1‑140 (MQ=255)                                                  tgtCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCaa                                                                            >  2:1253157/1‑140 (MQ=255)                                                   gtCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAAt                                                                           <  1:852214/140‑1 (MQ=255)                                                   gtCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAAt                                                                           <  1:1059682/140‑1 (MQ=255)                                                     cTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAAt                                                                           >  2:843329/1‑138 (MQ=255)                                                     cTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAAt                                                                           <  1:843329/138‑1 (MQ=255)                                                      tttACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCgg                                                                        >  1:1164058/1‑140 (MQ=255)                                                        tACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGgcg                                                                      >  2:585366/1‑140 (MQ=255)                                                        tACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGgcg                                                                      <  1:766876/140‑1 (MQ=255)                                                          ccGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCt                                                                    >  2:3365/1‑140 (MQ=255)                                                          ccGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCt                                                                    <  2:690353/140‑1 (MQ=255)                                                          ccGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCt                                                                    <  2:737847/140‑1 (MQ=255)                                                            gCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTgg                                                                  >  2:146475/1‑140 (MQ=255)                                                             cAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGc                                                                 <  1:438971/140‑1 (MQ=255)                                                              aaGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGcc                                                                >  2:320704/1‑140 (MQ=255)                                                              aaGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGcc                                                                <  2:1254905/140‑1 (MQ=255)                                                              aaGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGcc                                                                <  1:422138/140‑1 (MQ=255)                                                              aaGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGcc                                                                <  1:320704/140‑1 (MQ=255)                                                              aaGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGc                                                                 >  2:414203/1‑139 (MQ=255)                                                              aaGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGc                                                                 <  1:414203/139‑1 (MQ=255)                                                               agagGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCt                                                                    >  1:194858/1‑135 (MQ=255)                                                               agagGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCt                                                                    <  2:194858/135‑1 (MQ=255)                                                                     aCGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAAt                                                         <  2:1164058/140‑1 (MQ=255)                                                                     aCGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAAt                                                         <  2:752866/140‑1 (MQ=255)                                                                     aCGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAAt                                                         <  1:1069945/140‑1 (MQ=255)                                                                     aCGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAAt                                                         >  1:1084894/1‑140 (MQ=255)                                                                          tCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGt                                                    <  1:585366/140‑1 (MQ=255)                                                                           cGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGtt                                                   >  1:909620/1‑140 (MQ=255)                                                                                  aGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTg                                            >  2:391943/1‑140 (MQ=255)                                                                                    cGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGtt                                          >  1:403588/1‑140 (MQ=255)                                                                                       ggCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACg                                               <  2:584397/132‑1 (MQ=255)                                                                                       ggCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACg                                               >  1:584397/1‑132 (MQ=255)                                                                                         gaaTTGCTGCGTCCGGTTTCGGGTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCg                                     <  2:611341/139‑1 (MQ=255)                                                                                          aaTTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCgg                                    <  2:531090/140‑1 (MQ=255)                                                                                          aaTTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCgg                                    <  1:850512/140‑1 (MQ=255)                                                                                          aaTTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCgg                                    <  2:75170/140‑1 (MQ=255)                                                                                          aaTTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCgg                                    <  1:1179038/140‑1 (MQ=255)                                                                                            tttCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTc                                  <  1:146475/140‑1 (MQ=255)                                                                                                   gTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGTGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTAt                           >  1:1202468/1‑140 (MQ=255)                                                                                                      cggtttcggtttCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGt                                   >  1:708652/1‑129 (MQ=255)                                                                                                      cggtttcggtttCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGt                                   <  2:708652/129‑1 (MQ=255)                                                                                                      cggtttcggtttCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCa                        <  2:1148920/140‑1 (MQ=255)                                                                                                      cggtttcggtttCGCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCa                        <  2:181022/140‑1 (MQ=255)                                                                                                         tttcggtttcgCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCAcgc                     <  2:957307/140‑1 (MQ=255)                                                                                                         tttcggtttcgCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCAcgc                     <  2:479644/140‑1 (MQ=255)                                                                                                         tttcggtttcgCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCAcgc                     <  1:170472/140‑1 (MQ=255)                                                                                                              gtttcgCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGTGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCACGCCGCTg                <  2:1202468/140‑1 (MQ=255)                                                                                                               tttcgCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCACGCCGCTGc               <  2:1174433/140‑1 (MQ=255)                                                                                                                ttcgCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCACGCCGCTGcc              <  2:155863/140‑1 (MQ=255)                                                                                                                ttcgCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCACGCCGCTGcc              <  2:457634/140‑1 (MQ=255)                                                                                                                ttcgCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCACGCCGCTGcc              <  1:1253157/140‑1 (MQ=255)                                                                                                                  cgCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCACGCCGCTGcc              >  1:442750/1‑138 (MQ=255)                                                                                                                  cgCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCACGCCGCTGcc              <  2:442750/138‑1 (MQ=255)                                                                                                                  cgCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCACGCCGCTGCCAt            >  1:500536/1‑140 (MQ=255)                                                                                                                   gCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCACGCCGCTGCCATc           <  1:686008/140‑1 (MQ=255)                                                                                                                   gCTGTAAAGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAATTTATCCACGCCGCTGCCATc           <  1:329888/140‑1 (MQ=255)                                                                                                                         aaGGTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCACGCCGCTGCCATCCTGTAc     >  1:1014331/1‑140 (MQ=255)                                                                                                                            gTTTTGTCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCACGCCGCTGCCATCCTGTACCCa  <  2:500536/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      TCGAGAATGCCGTGCGCGACGGGCAAATAGCGCAGAGAGAAATAGAGAGTGTCTTTACCGCAAGAGGAACGTTTCGCTAACAGCGAGGCAATTTCTGCGTCCGGTTTCGGTTTCGCTGTAAAGGTTTTGCCTTTGCCGGCGACAAAAAGTTCATAAACAACGCCGCTGGCAGGGGCGGGACTTTGGCAATCGGCGCTGGCCTGTAAATAACGTTCACGCTGTTCAGCGGTCAGTTTATCCACGCCGCTGCCATCCTGTACCCA  >  NC_000913/587098‑587360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑