Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	827,858	A→G	M375T (ATG→ACG)	ybhF ←	putative ABC exporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	827,858	0	A	G	100.0%	109.1 / NA	32	M375T (ATG→ACG)	ybhF	putative ABC exporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/14);  total (18/14)

GTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCG  >  NC_000913/827721‑827987                                                                                                                                          |                                                                                                                                  gTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCAcgtc                                                                                                                                 >  1:1224934/1‑140 (MQ=255) gTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCAcgtc                                                                                                                                 >  2:783395/1‑140 (MQ=255)                 aCCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCg                                                                                                                 >  1:213524/1‑140 (MQ=255)                            aaaaTTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCcgcg                                                                                                      <  2:1224934/140‑1 (MQ=255)                                     gcgcCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCGCCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGc                                                                                             <  1:783395/140‑1 (MQ=255)                                     gcgcCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGc                                                                                             <  1:1238395/140‑1 (MQ=255)                                                    gaTGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCaaaa                                                                              >  2:804978/1‑140 (MQ=255)                                                     aTGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCaaaaa                                                                             >  2:534302/1‑140 (MQ=255)                                                       gCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAAtc                                                                           <  2:130464/140‑1 (MQ=255)                                                           gcgcgcTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTccc                                                                       <  2:800356/140‑1 (MQ=255)                                                                    aCCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTaa                                                              <  1:534302/140‑1 (MQ=255)                                                                              tctTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGtt                                                    >  1:829395/1‑140 (MQ=255)                                                                              tctTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAAGCTCCCCACGTTTAACGGCAAAGtt                                                    <  2:213524/140‑1 (MQ=255)                                                                                tttCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGa                                                  >  1:803746/1‑140 (MQ=255)                                                                                     gATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTg                                                   >  1:47736/1‑134 (MQ=255)                                                                                     gATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTg                                                   <  2:47736/134‑1 (MQ=255)                                                                                        ccatccCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGAc                                                 >  2:1193847/1‑133 (MQ=255)                                                                                        ccatccCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGAc                                                 <  1:1193847/133‑1 (MQ=255)                                                                                        ccatccCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCg                                          >  2:116330/1‑140 (MQ=255)                                                                                         catccCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCgg                                         >  2:1040653/1‑140 (MQ=255)                                                                                            ccccAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTgg                                      >  1:925154/1‑140 (MQ=255)                                                                                              ccAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTggcg                                    <  1:344242/140‑1 (MQ=255)                                                                                                aGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTggcggc                                  >  1:193182/1‑140 (MQ=255)                                                                                                     cAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAAt                             <  2:803746/140‑1 (MQ=255)                                                                                                       gcgcCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGAt                                            <  2:656731/123‑1 (MQ=255)                                                                                                       gcgcCTGGCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGAt                                            >  1:656731/1‑123 (MQ=255)                                                                                                             ggCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAAt                     >  2:200188/1‑140 (MQ=255)                                                                                                             ggCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAAt                     >  2:292165/1‑140 (MQ=255)                                                                                                              gCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAAtt                    >  2:126954/1‑140 (MQ=255)                                                                                                              gCCGGAAGTCGGCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAAtt                    >  2:280115/1‑140 (MQ=255)                                                                                                                        ggCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTgg              >  1:98749/1‑136 (MQ=255)                                                                                                                        ggCACCAGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTgg              <  2:98749/136‑1 (MQ=255)                                                                                                                              aGCAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCttt    <  2:193182/140‑1 (MQ=255)                                                                                                                                cAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCg  <  1:116330/140‑1 (MQ=255)                                                                                                                                cAAACCGCACGTCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCg  >  1:896716/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                  GTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCG  >  NC_000913/827721‑827987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑