Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,041,989	A→G	intergenic (+74/+41)	appA → / ← etk	periplasmic phosphoanhydride phosphatase/multiple inositol‑polyphosphate phosphatase/protein‑tyrosine kinase Etk

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,041,989	0	A	G	100.0%	163.3 / NA	48	intergenic (+74/+41)	appA/etk	periplasmic phosphoanhydride phosphatase/multiple inositol‑polyphosphate phosphatase/protein‑tyrosine kinase Etk
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/23);  total (25/23)

TCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATCACACCATTGAGGATAGC  >  NC_000913/1041856‑1042119                                                                                                                                      |                                                                                                                                   tCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGaa                                                                                                                              <  1:1143361/140‑1 (MQ=255)     tGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAgg                                                                                                                          >  1:464952/1‑140 (MQ=255)      ggCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGc                                                                                                                         >  2:193246/1‑140 (MQ=255)           gTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCa                                                                                                                    <  2:464952/140‑1 (MQ=255)             tttACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCAcg                                                                                                                  >  2:1030589/1‑140 (MQ=255)                                aaGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCa                                                                                               >  2:845317/1‑140 (MQ=255)                                    aCGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGttt                                                                                           >  1:558171/1‑140 (MQ=255)                                        aTACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTAct                                                                                       <  1:845317/140‑1 (MQ=255)                                          aCCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTActct                                                                                     >  1:926179/1‑140 (MQ=255)                                               cGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTtctc                                                                                >  2:805663/1‑140 (MQ=255)                                                gTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCg                                                                               >  1:689093/1‑140 (MQ=255)                                                  gCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGACCTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGa                                                                             <  1:1030589/140‑1 (MQ=255)                                                  gCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGa                                                                             >  1:1087528/1‑140 (MQ=255)                                                  gCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGa                                                                             <  2:558171/140‑1 (MQ=255)                                                    aGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGt                                                                           <  2:1171481/140‑1 (MQ=255)                                                    aGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGt                                                                           <  1:694769/140‑1 (MQ=255)                                                    aGTTTGTAATCCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGt                                                                           >  2:1057590/1‑140 (MQ=255)                                                      tttGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTaa                                                                         <  1:312888/140‑1 (MQ=255)                                                                aTAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGa                                                                             >  1:608798/1‑126 (MQ=255)                                                                aTAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGa                                                                             <  2:608798/126‑1 (MQ=255)                                                                aTAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGt                                                               >  2:280305/1‑140 (MQ=255)                                                                aTAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGt                                                               >  1:1207232/1‑140 (MQ=255)                                                                         agCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTtat                                                      >  1:454019/1‑140 (MQ=255)                                                                         agCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTtat                                                      <  1:193246/140‑1 (MQ=255)                                                                              tCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCg                                                 >  1:572587/1‑140 (MQ=255)                                                                              tCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCg                                                 >  1:321954/1‑140 (MQ=255)                                                                               cAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGt                                                <  1:1057590/140‑1 (MQ=255)                                                                                  ttACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAAc                                             >  1:584471/1‑140 (MQ=255)                                                                                        gAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAg                                       <  2:572587/140‑1 (MQ=255)                                                                                        gAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAg                                       <  1:805663/140‑1 (MQ=255)                                                                                              tctGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGc                                 >  2:1247738/1‑140 (MQ=255)                                                                                                  aGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGgc                             >  2:384798/1‑140 (MQ=255)                                                                                                   ggCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGgcg                            <  1:706162/140‑1 (MQ=255)                                                                                                   ggCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGgcg                            >  2:331462/1‑140 (MQ=255)                                                                                                   ggCTGATGACAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGgcg                            <  1:280305/140‑1 (MQ=255)                                                                                                       gatgaCAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGtt                        >  1:907077/1‑140 (MQ=255)                                                                                                       gatgaCAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGtt                        >  2:445511/1‑140 (MQ=255)                                                                                                        atgaCAAACGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGttt                       >  2:620327/1‑140 (MQ=255)                                                                                                             aaaCGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATca                  <  2:907077/140‑1 (MQ=255)                                                                                                               aCGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATcaca                <  1:384798/140‑1 (MQ=255)                                                                                                               aCGAAGAACTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATcaca                <  1:331462/140‑1 (MQ=255)                                                                                                                     aaCTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATCACACCATTg          <  2:584471/140‑1 (MQ=255)                                                                                                                     aaCTGTCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATCACACCATTg          <  1:620327/140‑1 (MQ=255)                                                                                                                          tCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATcaca                <  2:504277/129‑1 (MQ=255)                                                                                                                          tCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATcaca                >  1:504277/1‑129 (MQ=255)                                                                                                                          tCTAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATCACACCATTGAGGAt     >  1:614283/1‑140 (MQ=255)                                                                                                                            tAATGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATCACACCATTGATGATAg   <  1:1247738/140‑1 (MQ=255)                                                                                                                             aaTGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATCACACCATTGAGGATAgc  <  2:614283/140‑1 (MQ=255)                                                                                                                             aaTGCGTAGGCCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATCACACCATTGAGGATAgc  <  2:454019/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   TCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATCACACCATTGAGGATAGC  >  NC_000913/1041856‑1042119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑