Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,242,448	A→G	R211R (CGT→CGC)	ldcA ←	murein L,D‑carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,242,448	0	A	G	100.0%	292.9 / NA	82	R211R (CGT→CGC)	ldcA	murein L,D‑carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/51);  total (31/51)

CGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGA  >  NC_000913/1242311‑1242583                                                                                                                                          |                                                                                                                                        cGGGAACGCCAAAACGCGGACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCg                                                                                                                                       >  2:1256476/1‑140 (MQ=255)      aCGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGa                                                                                                                                  <  2:251157/140‑1 (MQ=255)      aCGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGa                                                                                                                                  <  2:1148081/140‑1 (MQ=255)      aCGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGa                                                                                                                                  <  1:591012/140‑1 (MQ=255)              aCGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGaaa                                                                                                                          <  1:752217/140‑1 (MQ=255)                   tACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTg                                                                                                                     <  1:845939/140‑1 (MQ=255)                   tACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTg                                                                                                                     <  2:763598/140‑1 (MQ=255)                    acacTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGc                                                                                                                    <  2:86952/140‑1 (MQ=255)                     cacTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCt                                                                                                                   <  1:176760/140‑1 (MQ=255)                          aCTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGaaa                                                                                                                          >  2:600641/1‑128 (MQ=255)                          aCTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGaaa                                                                                                                          <  1:600641/128‑1 (MQ=255)                          aCTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTa                                                                                                              >  2:1022072/1‑140 (MQ=255)                                ggTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCt                                                                                                        >  1:616566/1‑140 (MQ=255)                                ggTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCt                                                                                                        <  2:980754/140‑1 (MQ=255)                                   tGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTcc                                                                                                     <  1:647922/140‑1 (MQ=255)                                   tGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTcc                                                                                                     <  1:1103833/140‑1 (MQ=255)                                     tAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAg                                                                                                   <  1:710377/140‑1 (MQ=255)                                     tAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAg                                                                                                   <  2:616566/140‑1 (MQ=255)                                      aaCCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAg                                                                                                   >  2:33720/1‑139 (MQ=255)                                      aaCCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAg                                                                                                   <  1:33720/139‑1 (MQ=255)                                      aaCCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGc                                                                                                  >  1:803708/1‑140 (MQ=255)                                        ccTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCAc                                                                                                <  1:1022072/140‑1 (MQ=255)                                          tGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAg                                                                                                   <  2:1182505/135‑1 (MQ=255)                                          tGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAg                                                                                                   >  1:1182505/1‑135 (MQ=255)                                            cGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGa                                                                                            >  1:1002148/1‑140 (MQ=255)                                            cGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGa                                                                                            >  1:467077/1‑140 (MQ=255)                                               cATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATa                                                                                         <  2:891061/140‑1 (MQ=255)                                                      aTTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTtct                                                                                  <  1:160100/140‑1 (MQ=255)                                                      aTTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTtct                                                                                  >  1:582189/1‑140 (MQ=255)                                                      aTTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTtct                                                                                  >  2:80109/1‑140 (MQ=255)                                                        tGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCa                                                                                >  2:1099461/1‑140 (MQ=255)                                                         gggCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCaa                                                                               >  2:1138499/1‑140 (MQ=255)                                                         gggCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCaa                                                                               >  2:347348/1‑140 (MQ=255)                                                          ggCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAAt                                                                              >  1:144807/1‑140 (MQ=255)                                                            cGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAAttt                                                                            >  1:73975/1‑140 (MQ=255)                                                             gTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAAtttt                                                                           >  2:1164785/1‑140 (MQ=255)                                                              tgctgcCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAAttttt                                                                          >  1:311449/1‑140 (MQ=255)                                                              tgctgcCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAAttttt                                                                          >  2:920270/1‑140 (MQ=255)                                                              tgctgcCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAAttttt                                                                          >  1:287907/1‑140 (MQ=255)                                                              tgctgcCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAAttttt                                                                          >  2:1002915/1‑140 (MQ=255)                                                                   ccGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCAt                                                                     <  2:144807/140‑1 (MQ=255)                                                                   ccGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCAt                                                                     <  1:1002915/140‑1 (MQ=255)                                                                   ccGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCAt                                                                     <  1:347348/140‑1 (MQ=255)                                                                   ccGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCAt                                                                     <  1:728067/140‑1 (MQ=255)                                                                   ccGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCAt                                                                     <  1:1164785/140‑1 (MQ=255)                                                                   ccGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCACGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCAt                                                                     <  2:137369/140‑1 (MQ=255)                                                                   ccGCTAAAGCTACCGAGAATAATCGCCTTCTAACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCAt                                                                     <  2:1221866/140‑1 (MQ=255)                                                                     gCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATcc                                                                   <  1:643062/140‑1 (MQ=255)                                                                      cTAAAGCTACCGAGAATCATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTATTATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                  <  2:1120415/140‑1 (MQ=255)                                                                      cTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAGTATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                  <  1:143013/140‑1 (MQ=255)                                                                      cTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                  <  2:800529/140‑1 (MQ=255)                                                                      cTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                  <  2:803708/140‑1 (MQ=255)                                                                      cTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                  <  2:56850/140‑1 (MQ=255)                                                                      cTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                  <  1:1256476/140‑1 (MQ=255)                                                                      cTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                  <  1:1099461/140‑1 (MQ=255)                                                                      cTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                  <  2:799138/140‑1 (MQ=255)                                                                      cTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                  <  2:311449/140‑1 (MQ=255)                                                                      cTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                  <  1:80109/140‑1 (MQ=255)                                                                      cTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                  <  1:920270/140‑1 (MQ=255)                                                                      cTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                  <  2:1005430/140‑1 (MQ=255)                                                                        aaaGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATcc                                                                   >  2:883383/1‑137 (MQ=255)                                                                        aaaGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATcc                                                                   <  1:883383/137‑1 (MQ=255)                                                                                cGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCg                                                        <  1:635934/140‑1 (MQ=255)                                                                                cGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCg                                                        >  1:151315/1‑140 (MQ=255)                                                                                 gagaATAATCGCCTTCTGACGTGGCAAAATTCCCGAATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGa                                                       <  2:886726/140‑1 (MQ=255)                                                                                         tCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTAcc                                                         <  1:51202/130‑1 (MQ=255)                                                                                         tCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTAcc                                                         >  2:51202/1‑130 (MQ=255)                                                                                                         cAAAATTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGAt                               <  2:467077/140‑1 (MQ=255)                                                                                                           aaaTTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTg                             <  1:535006/140‑1 (MQ=255)                                                                                                            aaTTCCCGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGc                            <  2:287907/140‑1 (MQ=255)                                                                                                                 ccGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTccc                       <  2:151315/140‑1 (MQ=255)                                                                                                                  cGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCTACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGcc                           >  1:1018470/1‑135 (MQ=255)                                                                                                                  cGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCTACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGcc                           <  2:1018470/135‑1 (MQ=255)                                                                                                                  cGCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTcccc                      >  2:1078690/1‑140 (MQ=255)                                                                                                                   gCATGATAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCca                     <  2:582189/140‑1 (MQ=255)                                                                                                                        aTAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCca                     <  1:318514/135‑1 (MQ=255)                                                                                                                        aTAGAGCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCca                     >  2:318514/1‑135 (MQ=255)                                                                                                                            agCTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGcc            >  2:902800/1‑140 (MQ=255)                                                                                                                              cTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCtt          <  2:402341/140‑1 (MQ=255)                                                                                                                              cTGCAACAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCtt          >  1:402341/1‑140 (MQ=255)                                                                                                                                   aCAGCATGCGTTCGACCCGGAAAGGGTGCTCGTTAACATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGcc     >  2:341034/1‑140 (MQ=255)                                                                                                                                      gCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGa  >  2:549330/1‑140 (MQ=255)                                                                                                                                      gCATGCGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGa  >  2:398316/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGA  >  NC_000913/1242311‑1242583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑