Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,318,580	A→G	V403V (GTT→GTC)	trpC ←	fused indole‑3‑glycerol phosphate synthase/phosphoribosylanthranilate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,318,580	0	A	G	100.0%	179.4 / NA	51	V403V (GTT→GTC)	trpC	fused indole‑3‑glycerol phosphate synthase/phosphoribosylanthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/29);  total (22/29)

AAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCACCG  >  NC_000913/1318449‑1318703                                                                                                                                    |                                                                                                                            aaaaCCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGCCTCTACAGCAAAATTAAAATCAAGCCCGGCGCAGCCGGTTTGTGCCGCTCCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGcc                                                                                                                     <  2:411148/140‑1 (MQ=255)    aCCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAg                                                                                                                  >  2:858713/1‑140 (MQ=255)     ccGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGc                                                                                                                 >  1:7744/1‑140 (MQ=255)      cGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCg                                                                                                                >  1:1092766/1‑140 (MQ=255)      cGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCg                                                                                                                >  1:752746/1‑140 (MQ=255)           ccAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGa                                                                                                           <  2:12037/140‑1 (MQ=255)           ccAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGa                                                                                                           <  2:1092766/140‑1 (MQ=255)           ccAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGa                                                                                                           <  2:1183156/140‑1 (MQ=255)           ccAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGa                                                                                                           <  2:30646/140‑1 (MQ=255)           ccAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGa                                                                                                           <  2:572304/140‑1 (MQ=255)                aGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCAtt                                                                                                      <  2:1073034/140‑1 (MQ=255)                    gTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAAt                                                                                                  <  1:858713/140‑1 (MQ=255)                    gTGCCTCTTTGATGCCCGTCTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAAt                                                                                                  >  2:698236/1‑140 (MQ=255)                     tGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCAtt                                                                                                      >  1:917933/1‑135 (MQ=255)                     tGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCAtt                                                                                                      <  2:917933/135‑1 (MQ=255)                     tGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATa                                                                                                 >  1:787242/1‑140 (MQ=255)                         tCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGa                                                                                             >  2:988662/1‑140 (MQ=255)                          cTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGAc                                                                                            <  2:752746/140‑1 (MQ=255)                          cTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGAc                                                                                            <  1:988662/140‑1 (MQ=255)                                tGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGAc                                                                                            <  1:782355/134‑1 (MQ=255)                                tGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGAc                                                                                            >  2:782355/1‑134 (MQ=255)                                    cGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAAc                                                                                  >  2:61397/1‑140 (MQ=255)                                     ggTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACg                                                                                 >  2:433843/1‑140 (MQ=255)                                      gTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTc                                                                                       <  1:678084/133‑1 (MQ=255)                                      gTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTc                                                                                       >  2:678084/1‑133 (MQ=255)                                      gTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGt                                                                                >  2:1068854/1‑140 (MQ=255)                                      gTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGt                                                                                <  2:7744/140‑1 (MQ=255)                                      gTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGt                                                                                >  1:568875/1‑140 (MQ=255)                                       ttGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGtt                                                                               >  1:400308/1‑140 (MQ=255)                                        tGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCAATTGACCATTTAATAGTGACCAGTCAAAACGTTg                                                                              <  1:729904/140‑1 (MQ=255)                                          cGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGcc                                                                            <  1:61397/140‑1 (MQ=255)                                          cGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGcc                                                                            <  2:875948/140‑1 (MQ=255)                                          cGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGcc                                                                            <  1:1068854/140‑1 (MQ=255)                                               ctACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCtt                                                                       <  1:939049/140‑1 (MQ=255)                                                tACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTc                                                                      <  1:753403/140‑1 (MQ=255)                                                        aaTTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTgg                                                              >  2:947574/1‑140 (MQ=255)                                                        aaTTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTgg                                                              >  2:188195/1‑140 (MQ=255)                                                          ttAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGcc                                                            >  2:600855/1‑140 (MQ=255)                                                          ttAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGcc                                                            >  2:1184864/1‑140 (MQ=255)                                                           tAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCg                                                           <  1:85736/140‑1 (MQ=255)                                                               aTCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGt                                                       <  1:747129/140‑1 (MQ=255)                                                                  aaGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTa                                                    <  1:600855/140‑1 (MQ=255)                                                                  aaGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTa                                                    >  1:1080929/1‑140 (MQ=255)                                                                    gTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTaaa                                                  <  2:300405/140‑1 (MQ=255)                                                                                cGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCa                                      <  1:433843/140‑1 (MQ=255)                                                                                cGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCa                                      <  1:188195/140‑1 (MQ=255)                                                                                 ggTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCaa                                     <  1:1184864/140‑1 (MQ=255)                                                                                                      gTTATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCgcgggcgg                >  1:1202779/1‑140 (MQ=255)                                                                                                       ttATCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGcgggcggg               <  2:568875/140‑1 (MQ=255)                                                                                                         aTCTGCGCCTAAGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCa             >  1:950496/1‑140 (MQ=255)                                                                                                                   aaGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCCAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCAcc   >  2:765395/1‑140 (MQ=255)                                                                                                                    aGCCCCCCGCCAGCAGGACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCACCg  <  2:1080929/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                            AAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAACGTTGCCAAGCGATTGACCATTTAATAGTGACCAGTCAAAACGTTGCCCGCTTCCACCCTGGCCGTTGTCTAAAACATATTTATCAACGTGCTGAAACTCGCGGGCGGGCAGGGTTTCACCG  >  NC_000913/1318449‑1318703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑