Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,320,484	C→T	G301R (GGG→AGG)	trpD ←	anthranilate synthase subunit TrpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,320,484	0	C	T	100.0%	91.2 / NA	27	G301R (GGG→AGG)	trpD	anthranilate synthase subunit TrpD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/13);  total (14/13)

CACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCCACAGGCCGCGGCGACAAACGCACTGGCGGTAGAAATATTGATACTGTTGCTGCCGTCACCGCCAGTACCGACGATATCAGCA  >  NC_000913/1320352‑1320566                                                                                                                                     |                                                                                   cacCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGcc                                                                             >  2:465687/1‑140 (MQ=255) cacCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGcc                                                                             >  2:19237/1‑140 (MQ=255)      aaCTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCgcggc                                                                        >  2:318026/1‑140 (MQ=255)       aCTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATATCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCgcggcg                                                                       >  2:1162875/1‑140 (MQ=255)       aCTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCcg                                                                            <  1:1092915/135‑1 (MQ=255)       aCTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCcg                                                                            >  2:258173/1‑135 (MQ=255)       aCTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCcg                                                                            <  1:258173/135‑1 (MQ=255)       aCTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCcg                                                                            >  2:1092915/1‑135 (MQ=255)        cTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGa                                                                      >  2:1099757/1‑140 (MQ=255)            tCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACaaa                                                                  >  2:1074162/1‑140 (MQ=255)            tCCAGCGCCTGGCGCGATTTATCAGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACaaa                                                                  >  2:557579/1‑140 (MQ=255)                 cgcCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCAc                                                             >  1:146792/1‑140 (MQ=255)                  gcCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATATCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACt                                                            <  1:1162875/140‑1 (MQ=255)                   ccTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTg                                                           >  1:589122/1‑140 (MQ=255)                   ccTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTg                                                           <  2:1224868/140‑1 (MQ=255)                       gcgcGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTGGCgg                                                       <  1:465687/140‑1 (MQ=255)                         gcgATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGTCGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTGGCGGTa                                                     <  1:1099757/140‑1 (MQ=255)                          cgATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTGGCGGTAg                                                    <  1:318026/140‑1 (MQ=255)                           gATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTGGCGGTAGa                                                   <  2:1116098/140‑1 (MQ=255)                           gATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTGGCGGTAGa                                                   <  1:464161/140‑1 (MQ=255)                            aTTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTGGCGGTAGaa                                                  >  1:825079/1‑140 (MQ=255)                              ttATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTGGCGGTAGAAat                                                <  2:1153145/140‑1 (MQ=255)                              ttATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTGGCGGTAGAAat                                                <  1:1074162/140‑1 (MQ=255)                              ttATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTGGCGGTAGAAat                                                <  2:141943/140‑1 (MQ=255)                                         cATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTGGCGGTAGAAATATTGATACTGt                                     >  1:1103857/1‑140 (MQ=255)                                              cAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTGGCGGTAGAAATATTGATACTGTtgctg                                <  1:557579/140‑1 (MQ=255)                                                  aTTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTGGCGGTAGAAATATTGATACTGTTGCTGCCGt                            >  1:332012/1‑140 (MQ=255)                                                       ccccGACCGCCGCCAGCAGATCGGACGAACAAGTTTTACGGGAGACGCTAGGTTTGCGGTTTTCCCCCCCTTTCAGCCTACAGGCCGCGGCGAAAAACGCACGGGGGGTAGAAATATTGATACTGTTGCTGCCGTCACCg                       <  2:589122/138‑1 (MQ=255)                                                            aaCGCCGCCAGCAGATCGGACGAACCAGATCTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCTACAGGCCGCGGCGACAAACGCACTGGCGGTAGAAATATTTATACTGTTGCTGCCGTCACCCCCAGt                  >  1:1251949/1‑140 (MQ=255)                                                                            gggACAAACCAGTTTTACGGGAGCCGCTAGGGTTCCGGTTTTCCCCCCTTTTCACCCTCCAGGCCGCGGCGAAAACCCCCGTGGCGGTAAAAATATTAATACTGTTGCTGCCGTCACCGCCAGTACCGACGATATCAGCa  <  2:482060/139‑1 (MQ=255)                                                                                                                                     |                                                                                   CACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATTTACTGGAGACGCTACGGTTGCCGTGTTTCGCCACTTTCAGCCCACAGGCCGCGGCGACAAACGCACTGGCGGTAGAAATATTGATACTGTTGCTGCCGTCACCGCCAGTACCGACGATATCAGCA  >  NC_000913/1320352‑1320566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑