Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,548,132	G→A	A244A (GCG→GCA)	fdnG →	formate dehydrogenase N subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,548,132	0	G	A	100.0%	154.6 / NA	44	A244A (GCG→GCA)	fdnG	formate dehydrogenase N subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/20);  total (24/20)

CCAACGGTAGCAAGTCTTGCTCCAACATTTGGTCGCGGTGCGATGACCAACCACTGGGTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGC  >  NC_000913/1547995‑1548267                                                                                                                                          |                                                                                                                                        ccAACGGTAGCAAGTCTTGCTCCAACATTTGGTCGCGGTGCGATGACCAACCACTGGGTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAAt                                                                                                                                       >  1:456375/1‑140 (MQ=255)          gCAAGTCTTGCTCCAACATTTGGTCGCGGTGCGATGACCAACCACTGGGTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGaa                                                                                                                              >  2:369260/1‑140 (MQ=255)                cTTGCTCCAACATTTGGTCGCGGTGCGATGACCAACCACTGGGTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAaacaa                                                                                                                        >  1:80991/1‑140 (MQ=255)                  tGCTCCAACATTTGGTCGCGGTGCGATGACCAACCACTGGGTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAaacaaca                                                                                                                      >  1:12552/1‑140 (MQ=255)                    cTCCAACATTTGGTCGCGGTGCGATGACCAACCACTGGGTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAaacaacaac                                                                                                                    >  2:27322/1‑140 (MQ=255)                        aaCATTTGGTCGCGGTGCGATGACCAACCACTGGGTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAacgacg                                                                                                                <  1:27322/140‑1 (MQ=255)                         aCATTTGGTCGCGGTGCGATGACCAACCACTGGGTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGc                                                                                                               >  2:1140988/1‑140 (MQ=255)                              tGGTCGCGGTGCGATGACCAACCACTGGGTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCt                                                                                                          >  1:60398/1‑140 (MQ=255)                                                   ccaCTGGGTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGtt                                                                                     >  1:125574/1‑140 (MQ=255)                                                         ggTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGttt                                                                                    <  1:263169/135‑1 (MQ=255)                                                         ggTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGttt                                                                                    >  2:263169/1‑135 (MQ=255)                                                         ggTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTAcgc                                                                               >  1:648622/1‑140 (MQ=255)                                                          gTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTAcgcg                                                                              <  2:12552/140‑1 (MQ=255)                                                             gATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTAc                                                                           >  2:1234352/1‑140 (MQ=255)                                                             gATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTAc                                                                           >  2:335459/1‑140 (MQ=255)                                                              atatCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTAcc                                                                          >  2:516686/1‑140 (MQ=255)                                                               tatCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCg                                                                         <  2:456375/140‑1 (MQ=255)                                                                 tCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCt                                                                       >  2:928750/1‑140 (MQ=255)                                                                  cAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCtt                                                                      <  2:125574/140‑1 (MQ=255)                                                                     aaaCGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCtg                                                                   >  1:510671/1‑140 (MQ=255)                                                                       aCGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCtgtg                                                                 <  2:510671/140‑1 (MQ=255)                                                                         gCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGc                                                               <  1:489050/140‑1 (MQ=255)                                                                                  gtgatggtgatgGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTa                                                      <  1:369260/140‑1 (MQ=255)                                                                                   tgatggtgatggGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATAtttta                                                     >  2:1256700/1‑138 (MQ=255)                                                                                   tgatggtgatggGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTAc                                                     >  2:127279/1‑140 (MQ=255)                                                                                   tgatggtgatggGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTAc                                                     >  2:639160/1‑140 (MQ=255)                                                                                    gatggtgatggGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTAcg                                                    <  2:510641/140‑1 (MQ=255)                                                                                         tgatggGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTAcgcg                                                  >  2:1040746/1‑137 (MQ=255)                                                                                         tgatggGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTAcgcg                                                  <  1:1040746/137‑1 (MQ=255)                                                                                           atggGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTac                                             >  1:303413/1‑139 (MQ=255)                                                                                            tggGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTAtt                                            <  1:639160/140‑1 (MQ=255)                                                                                                cGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGtt                                        <  2:648622/140‑1 (MQ=255)                                                                                                  gTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTcc                                      <  1:1140988/140‑1 (MQ=255)                                                                                                          gctgAAGCGCATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGa                              <  2:80991/140‑1 (MQ=255)                                                                                                                 cgcATCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACg                       <  2:60398/140‑1 (MQ=255)                                                                                                                     tCCCGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACCCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTcc                   >  1:54420/1‑140 (MQ=255)                                                                                                                      cccGTCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTACTCGTTCCGGTACGGACATTACGTTCCt                  <  2:303413/140‑1 (MQ=255)                                                                                                                          tCGGTTTCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCt              >  2:1042041/1‑140 (MQ=255)                                                                                                                               ttCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGt         <  1:144778/140‑1 (MQ=255)                                                                                                                                tCCGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGtt        <  1:516686/140‑1 (MQ=255)                                                                                                                                 ccGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGttt       <  1:928750/140‑1 (MQ=255)                                                                                                                                 ccGCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGttt       >  1:256668/1‑140 (MQ=255)                                                                                                                                   gCTGGGCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTg     <  1:342656/140‑1 (MQ=255)                                                                                                                                      gggCAATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTAAGGTCCTGTCTGGCGGTTTtcgt  >  1:132835/1‑139 (MQ=255)                                                                                                                                          |                                                                                                                                        CCAACGGTAGCAAGTCTTGCTCCAACATTTGGTCGCGGTGCGATGACCAACCACTGGGTGGATATCAAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGTTTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCCCGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGC  >  NC_000913/1547995‑1548267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑