Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,727,492	G→A	G278G (GGG→GGA)	nemA →	N‑ethylmaleimide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,727,492	0	G	A	100.0%	197.7 / NA	56	G278G (GGG→GGA)	nemA	N‑ethylmaleimide reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/26);  total (30/26)

TTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATT  >  NC_000913/1727356‑1727626                                                                                                                                         |                                                                                                                                       ttCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCGCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGt                                                                                                                                     <  2:773716/140‑1 (MQ=255) ttCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGt                                                                                                                                     <  1:712986/140‑1 (MQ=255) ttCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGt                                                                                                                                     <  1:219578/140‑1 (MQ=255) ttCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGt                                                                                                                                     <  2:163630/140‑1 (MQ=255)   cgcgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCGCTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGa                                                                                                                                   >  2:137874/1‑140 (MQ=255)    gcgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCGCTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGaa                                                                                                                                  <  1:137874/140‑1 (MQ=255)    gcgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGaa                                                                                                                                  <  1:671886/140‑1 (MQ=255)    gcgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGaa                                                                                                                                  <  1:931212/140‑1 (MQ=255)                tCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGt                                                                                                                                     <  2:291176/125‑1 (MQ=255)                tCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGt                                                                                                                                     >  1:291176/1‑125 (MQ=255)                       tttCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTcc                                                                                                               <  1:41540/140‑1 (MQ=255)                              aaCACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGaaaa                                                                                                        >  2:1080634/1‑140 (MQ=255)                              aaCACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGaaaa                                                                                                        >  2:298924/1‑140 (MQ=255)                                   aGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAc                                                                                                   >  1:1006099/1‑140 (MQ=255)                                   aGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAc                                                                                                   >  2:202745/1‑140 (MQ=255)                                     aTAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAcgc                                                                                                 >  1:925119/1‑140 (MQ=255)                                      tAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAcgcg                                                                                                <  2:612151/140‑1 (MQ=255)                                        aCGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGcc                                                                                              >  1:1244554/1‑140 (MQ=255)                                          ggCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCg                                                                                            >  2:357597/1‑140 (MQ=255)                                           gCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGt                                                                                           >  1:500280/1‑140 (MQ=255)                                              cGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTc                                                                                        >  1:1049972/1‑140 (MQ=255)                                                aaTGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCa                                                                                      <  1:901283/140‑1 (MQ=255)                                                aaTGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCa                                                                                      <  1:357597/140‑1 (MQ=255)                                                aaTGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCa                                                                                      <  2:500280/140‑1 (MQ=255)                                                            gATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTAt                                                                          <  2:1049972/140‑1 (MQ=255)                                                              tGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCg                                                                        >  2:337197/1‑140 (MQ=255)                                                                   tGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                                                                   <  2:1115769/140‑1 (MQ=255)                                                                     tATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                                                                   >  2:1065172/1‑138 (MQ=255)                                                                     tATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                                                                   <  1:1065172/138‑1 (MQ=255)                                                                       tCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGgcg                                                                     >  2:266450/1‑134 (MQ=255)                                                                       tCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGgcg                                                                     <  1:266450/134‑1 (MQ=255)                                                                          gATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCAt                                                            <  1:337197/140‑1 (MQ=255)                                                                           aTTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATa                                                           <  1:298924/140‑1 (MQ=255)                                                                             tGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATaca                                                         >  1:603930/1‑140 (MQ=255)                                                                             tGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATaca                                                         >  1:446369/1‑140 (MQ=255)                                                                              gAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATacac                                                        <  2:925119/140‑1 (MQ=255)                                                                               aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGgcg                                                                     >  2:1227477/1‑126 (MQ=255)                                                                               aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGgcg                                                                     <  1:1227477/126‑1 (MQ=255)                                                                               aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                                                                   <  1:820934/128‑1 (MQ=255)                                                                               aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATAcaca                                                       >  2:353970/1‑140 (MQ=255)                                                                               aacaacTGGGCAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                                                                   >  2:820934/1‑128 (MQ=255)                                                                                     tGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATa                                                           >  1:783089/1‑130 (MQ=255)                                                                                     tGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATa                                                           <  2:783089/130‑1 (MQ=255)                                                                                     tGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGaa                                                 >  1:352682/1‑140 (MQ=255)                                                                                                 gCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACg                                     <  1:202745/140‑1 (MQ=255)                                                                                                          aTCTGCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGc                            >  1:61922/1‑140 (MQ=255)                                                                                                                 tatGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGGTCGGCAAAGGGt                     >  1:1262250/1‑140 (MQ=255)                                                                                                                 tatGTCAGAACCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGt                     >  1:318201/1‑140 (MQ=255)                                                                                                                         aaCCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGAt             >  1:1157968/1‑140 (MQ=255)                                                                                                                         aaCCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGAt             >  2:327523/1‑140 (MQ=255)                                                                                                                         aaCCAGATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGAt             <  2:318201/140‑1 (MQ=255)                                                                                                                              gATTGGGCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGt        >  1:268338/1‑140 (MQ=255)                                                                                                                                   ggCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCAt   >  1:1120377/1‑140 (MQ=255)                                                                                                                                   ggCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCAt   >  1:816523/1‑140 (MQ=255)                                                                                                                                    gCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGGTCGGCAAAGGGTTAATTGATGCGGTGGCAtt  <  2:1262250/140‑1 (MQ=255)                                                                                                                                    gCGGGAGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCAtt  >  2:634761/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATT  >  NC_000913/1727356‑1727626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑