Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	135,657	T→C	T270A (ACG→GCG)	speE ←	spermidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	135,657	0	T	C	100.0%	236.4 / NA	67	T270A (ACG→GCG)	speE	spermidine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/32);  total (35/32)

TAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGTCATGATACCGCCGTAATAGG  >  NC_000913/135521‑135791                                                                                                                                         |                                                                                                                                       tAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATg                                                                                                                                     >  2:1124820/1‑140 (MQ=255) tAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATg                                                                                                                                     <  1:932345/140‑1 (MQ=255) tAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATg                                                                                                                                     <  1:874423/140‑1 (MQ=255)     tACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGAtt                                                                                                                                 >  2:375676/1‑140 (MQ=255)      aCAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTg                                                                                                                                <  1:221823/140‑1 (MQ=255)        aaaaaCTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGcc                                                                                                                              >  1:386962/1‑140 (MQ=255)        aaaaaCTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGcc                                                                                                                              <  1:476264/140‑1 (MQ=255)         aaaaCTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGc                                                                                                                               >  1:1230574/1‑138 (MQ=255)         aaaaCTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGc                                                                                                                               <  2:1230574/138‑1 (MQ=255)          aaaCTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCgg                                                                                                                            >  2:159478/1‑140 (MQ=255)               cAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGt                                                                                                                       >  2:872728/1‑140 (MQ=255)                aGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGta                                                                                                                      >  1:1203391/1‑140 (MQ=255)                aGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGta                                                                                                                      >  1:1252876/1‑140 (MQ=255)                       tGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACgg                                                                                                               <  2:33777/140‑1 (MQ=255)                          tCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGG‑AAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCAtt                                                                                                           >  2:70441/1‑140 (MQ=255)                           cAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCAtt                                                                                                           <  2:386962/140‑1 (MQ=255)                                        ccATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGcc                                                                                                    >  1:244289/1‑134 (MQ=255)                                        ccATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGcc                                                                                                    <  2:244289/134‑1 (MQ=255)                                        ccATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGc                                                                                              >  2:943314/1‑140 (MQ=255)                                        ccATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGc                                                                                              >  1:737415/1‑140 (MQ=255)                                        ccATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGc                                                                                              >  1:322895/1‑140 (MQ=255)                                            gCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCgaga                                                                                          >  2:1216615/1‑140 (MQ=255)                                                 ttcagtttTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAAcg                                                                                     <  1:1216615/140‑1 (MQ=255)                                                    agtttTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACgcgc                                                                                  <  1:570395/140‑1 (MQ=255)                                                    agtttTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACgcgc                                                                                  <  2:1060321/140‑1 (MQ=255)                                                        ttttCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAgg                                                                                               >  1:1147838/1‑123 (MQ=255)                                                        ttttCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAgg                                                                                               <  2:1147838/123‑1 (MQ=255)                                                        ttttCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGa                                                                              <  2:322895/140‑1 (MQ=255)                                                          ttCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGaat                                                                            >  1:355161/1‑140 (MQ=255)                                                            cAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGaataa                                                                          <  2:671499/140‑1 (MQ=255)                                                                         tctcCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTgagag                                                             <  2:367478/140‑1 (MQ=255)                                                                             cTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATTCTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGGTgg                                                         >  2:1103202/1‑140 (MQ=255)                                                                                gggACGGCTGTGAAGCCAGTCAGTCTGGCCGAGACGGCGGTAAGGCCACAGCCGCCGCCCGGATTGCCGGCTTCGCATAACGGCATGTCAGCCCCCACGCGAGACAGCGCGCGGGGACAACCTCGGTTGAGAGAAGGCGt                                                      <  2:186022/139‑1 (MQ=255)                                                                                aGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGt                                                      <  1:98417/140‑1 (MQ=255)                                                                                aGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGt                                                      <  2:186023/140‑1 (MQ=255)                                                                                 ggACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTa                                                     <  2:1002784/140‑1 (MQ=255)                                                                                 ggACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTa                                                     <  2:909079/140‑1 (MQ=255)                                                                                   aCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAg                                                   <  1:559064/140‑1 (MQ=255)                                                                                   aCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAg                                                   <  2:714175/140‑1 (MQ=255)                                                                                   aCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAg                                                   <  1:159478/140‑1 (MQ=255)                                                                                    cGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAgg                                                  <  2:355161/140‑1 (MQ=255)                                                                                      gCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGcg                                                >  2:119432/1‑140 (MQ=255)                                                                                      gCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGcg                                                <  2:218143/140‑1 (MQ=255)                                                                                       cTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGcgt                                               >  2:406573/1‑140 (MQ=255)                                                                                       cTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGcgt                                               >  2:529882/1‑140 (MQ=255)                                                                                       cTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGcgt                                               >  1:581114/1‑140 (MQ=255)                                                                                       cTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGcgt                                               >  2:706998/1‑140 (MQ=255)                                                                                          tgAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAg                                                   >  1:1098573/1‑133 (MQ=255)                                                                                          tgAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAg                                                   <  2:1098573/133‑1 (MQ=255)                                                                                                cAGTGCGTCTTGCAGATACTGAGG‑AAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGt                                     <  1:70441/140‑1 (MQ=255)                                                                                                 aGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGt                                     >  1:745609/1‑140 (MQ=255)                                                                                                   tGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCg                                   >  2:572909/1‑140 (MQ=255)                                                                                                           gCAGATTCTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGGTGGCGTAAGGCGTCGTTATCTGTCGCCCATGCa                           <  1:1103202/140‑1 (MQ=255)                                                                                                           gCAGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCa                           <  1:872728/140‑1 (MQ=255)                                                                                                             aGATACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCaaa                         <  1:119432/140‑1 (MQ=255)                                                                                                                tACTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGt                      <  2:1203391/140‑1 (MQ=255)                                                                                                                 aCTGAGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGTc                     >  1:966711/1‑140 (MQ=255)                                                                                                                     aGGTAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGTCATGa                 >  2:783029/1‑140 (MQ=255)                                                                                                                      ggaaaGGAAAAAGTGGCGGCATGGATTGCGGGTTTTTAATAACGGCTTTTCAGGCAAGAGGCGAAAAAACGCGCTTAAATAATTTCGGTTGAGAGAGGGCGAAAGGCGTCTTTTTCTGTCGCCCATGAAAAAGTCATGAt                <  2:1098900/137‑1 (MQ=255)                                                                                                                        tAAGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGTCATGATAc              <  2:745609/140‑1 (MQ=255)                                                                                                                          aGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGTCATGATAccg            <  2:581114/140‑1 (MQ=255)                                                                                                                          aGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGTCATGATAccg            <  1:525489/140‑1 (MQ=255)                                                                                                                          aGGCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGTCATGATAccg            >  1:977392/1‑140 (MQ=255)                                                                                                                           ggCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATg                             >  2:571014/1‑122 (MQ=255)                                                                                                                           ggCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATg                             <  1:571014/122‑1 (MQ=255)                                                                                                                           ggCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGTCATGATAccgc           >  1:1252833/1‑140 (MQ=255)                                                                                                                           ggCAAAAGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGTCATGATAccgc           >  1:48490/1‑140 (MQ=255)                                                                                                                               aaaGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGTCATGAt                >  1:539063/1‑131 (MQ=255)                                                                                                                               aaaGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGTCATGATACCGCCGTa       >  2:868774/1‑140 (MQ=255)                                                                                                                               aaaGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGAAAAAGTCATGAt                <  2:539063/131‑1 (MQ=255)                                                                                                                                 aGCTGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGTCATGATACCGCCGTAAt     >  2:364536/1‑140 (MQ=255)                                                                                                                                    tGCCGCATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGTCATGATACCGCCGTAATAgg  >  2:128049/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCGTAAGGCGTCGTTATCTGTCGCCCATGCAAAAGTCATGATACCGCCGTAATAGG  >  NC_000913/135521‑135791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑