Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,744,504	G→A	V350I (GTT→ATT)	mdtK →	multidrug efflux pump MdtK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,744,504	0	G	A	100.0%	260.0 / NA	73	V350I (GTT→ATT)	mdtK	multidrug efflux pump MdtK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/42);  total (31/42)

AACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACG  >  NC_000913/1744377‑1744635                                                                                                                                |                                                                                                                                    aaCGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACgctgg                                                                                                                         >  1:389235/1‑140 (MQ=255) aaCGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACgctgg                                                                                                                         >  2:889086/1‑140 (MQ=255) aaCGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACgctgg                                                                                                                         >  1:1018782/1‑140 (MQ=255)   cGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACgctggct                                                                                                                       <  1:388484/140‑1 (MQ=255)     cTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTgc                                                                                                                     <  1:1114452/140‑1 (MQ=255)       ggATGCGCAAACCGCTGCGCGGTCCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTgcgc                                                                                                                   <  1:889086/140‑1 (MQ=255)              cAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGAt                                                                                                            >  2:221760/1‑140 (MQ=255)              cAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGAt                                                                                                            >  2:658198/1‑140 (MQ=255)                       gcgcGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTggc                                                                                                   <  2:981889/140‑1 (MQ=255)                        cgcgGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCAtt                                                                                                                <  2:69233/126‑1 (MQ=255)                        cgcgGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCAtt                                                                                                                >  1:69233/1‑126 (MQ=255)                                  gCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATc                                                                                        >  2:678867/1‑140 (MQ=255)                                   cTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCa                                                                                       <  1:658198/140‑1 (MQ=255)                                   cTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCa                                                                                       <  1:221760/140‑1 (MQ=255)                                        ggtgggtgTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGAttt                                                                                  <  1:1139964/140‑1 (MQ=255)                                            ggtgTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGa                                                                              >  1:59947/1‑140 (MQ=255)                                            ggtgTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGa                                                                              >  2:637190/1‑140 (MQ=255)                                            ggtgTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGa                                                                              >  2:919381/1‑140 (MQ=255)                                            ggtgTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGa                                                                              >  2:1077464/1‑140 (MQ=255)                                             gtgtCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGAc                                                                             <  1:713762/140‑1 (MQ=255)                                                 cTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCaa                                                                         >  1:1233126/1‑140 (MQ=255)                                                    tATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATcc                                                                      <  1:678867/140‑1 (MQ=255)                                                    tATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATcc                                                                      <  1:992290/140‑1 (MQ=255)                                                    tATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATcc                                                                      >  2:992290/1‑140 (MQ=255)                                                    tATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATcc                                                                      <  1:508022/140‑1 (MQ=255)                                                    tATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATcc                                                                      <  1:1187322/140‑1 (MQ=255)                                                            cccTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGAtt                                                              <  2:1233126/140‑1 (MQ=255)                                                            cccTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGAtt                                                              >  2:811704/1‑140 (MQ=255)                                                            cccTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGAtt                                                              >  2:1158383/1‑140 (MQ=255)                                                            cccTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGAt                                                               >  2:801516/1‑139 (MQ=255)                                                            cccTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGAt                                                               <  1:801516/139‑1 (MQ=255)                                                             ccTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTg                                                             <  1:1124519/140‑1 (MQ=255)                                                              cTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTgg                                                            >  2:789192/1‑140 (MQ=255)                                                               tGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATAGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGc                                                           >  1:144496/1‑140 (MQ=255)                                                                 aCGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAg                                                         >  2:1260525/1‑140 (MQ=255)                                                                 aCGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAg                                                         >  2:775990/1‑140 (MQ=255)                                                                  cGGCCATTTTCACGGTTTCACTGCGGGAGCAAATAGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGt                                                        <  2:144496/140‑1 (MQ=255)                                                                      cATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGa                                                    <  1:1158383/140‑1 (MQ=255)                                                                           tCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTg                                               <  1:789192/140‑1 (MQ=255)                                                                           tCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTg                                               <  2:1045534/140‑1 (MQ=255)                                                                           tCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTg                                               <  1:500850/140‑1 (MQ=255)                                                                             aCGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCg                                             <  1:775990/140‑1 (MQ=255)                                                                             aCGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCg                                             <  1:737046/140‑1 (MQ=255)                                                                             aCGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCg                                             <  2:1018782/140‑1 (MQ=255)                                                                              cGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGt                                            <  1:637190/140‑1 (MQ=255)                                                                              cGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGt                                            <  1:919381/140‑1 (MQ=255)                                                                                  ttCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGtt                                        >  1:48955/1‑140 (MQ=255)                                                                                   tCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCg                                             >  1:920410/1‑134 (MQ=255)                                                                                   tCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCg                                             <  2:920410/134‑1 (MQ=255)                                                                                   tCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTta                                       <  2:48955/140‑1 (MQ=255)                                                                                     aCTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTtata                                     <  1:1260525/140‑1 (MQ=255)                                                                                           ggAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCg                                             <  1:840002/126‑1 (MQ=255)                                                                                           ggAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCg                                             >  2:840002/1‑126 (MQ=255)                                                                                           ggAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGt                                            >  1:220519/1‑127 (MQ=255)                                                                                           ggAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGt                                            <  1:511615/127‑1 (MQ=255)                                                                                           ggAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGt                                            >  2:511615/1‑127 (MQ=255)                                                                                           ggAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGt                                            <  2:220519/127‑1 (MQ=255)                                                                                                  aTCGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTc                        <  2:505506/140‑1 (MQ=255)                                                                                                    cGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCa                      <  1:66895/140‑1 (MQ=255)                                                                                                    cGCCCTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCa                      <  1:985293/140‑1 (MQ=255)                                                                                                       ccTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCAttt                   <  1:1067738/140‑1 (MQ=255)                                                                                                       ccTGTTGTACAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCAttt                   <  1:447422/140‑1 (MQ=255)                                                                                                                cAACGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTa          >  1:1139356/1‑140 (MQ=255)                                                                                                                 aaCGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTAc         <  1:1077464/140‑1 (MQ=255)                                                                                                                   cGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTcc                       >  2:418550/1‑124 (MQ=255)                                                                                                                   cGACAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTcc                       <  1:418550/124‑1 (MQ=255)                                                                                                                     aCAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCtat              <  2:268472/131‑1 (MQ=255)                                                                                                                     aCAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCtat              >  1:268472/1‑131 (MQ=255)                                                                                                                     aCAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTatat            <  1:333318/133‑1 (MQ=255)                                                                                                                     aCAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTatat            >  2:333318/1‑133 (MQ=255)                                                                                                                     aCAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCttt     <  2:1139356/140‑1 (MQ=255)                                                                                                                     aCAATCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCttt     >  2:302114/1‑140 (MQ=255)                                                                                                                        aTCCCGAGATTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACg  >  1:1155329/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                    AACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACG  >  NC_000913/1744377‑1744635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑