Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,852,745	T→C	H412R (CAT→CGT)	ydjE ←	putative transporter YdjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,852,745	0	T	C	100.0%	206.7 / NA	59	H412R (CAT→CGT)	ydjE	putative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/21);  total (38/21)

AATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGC  >  NC_000913/1852623‑1852877                                                                                                                             |                                                                                                                                     aaTTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCa                                                                                                                     >  1:442266/1‑140 (MQ=255) aaTTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCa                                                                                                                     <  2:1008021/140‑1 (MQ=255)        cTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACTGTGATCGA‑‑CCCATGACGTGTTAATAATGCCGCAAAGCCAt                                                                                                              >  2:1113866/1‑140 (MQ=255)        cTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCAt                                                                                                              <  2:603895/140‑1 (MQ=255)                  tctTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAg                                                                                                            >  1:361512/1‑132 (MQ=255)                  tctTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAg                                                                                                            <  2:361512/132‑1 (MQ=255)                  tctTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGAGTTAATAATGCCGCAACGCCATAGGGCGTGaa                                                                                                    >  2:546659/1‑140 (MQ=255)                    ttCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGa                                                                                                  <  2:930630/140‑1 (MQ=255)                     tCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGAc                                                                                                 >  1:15011/1‑140 (MQ=255)                     tCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGAc                                                                                                 >  2:35466/1‑140 (MQ=255)                         aCGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGcg                                                                                             >  1:1229339/1‑140 (MQ=255)                            acacCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGcgacg                                                                                          >  2:846778/1‑140 (MQ=255)                              acCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGAt                                                                                        >  2:134974/1‑140 (MQ=255)                                cTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGAtcc                                                                                      >  2:219861/1‑140 (MQ=255)                                cTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGAtcc                                                                                      >  1:1055930/1‑140 (MQ=255)                                   ccGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGAtccgtc                                                                                   >  1:1165985/1‑140 (MQ=255)                                        tttCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACg                                                                              <  2:1165985/140‑1 (MQ=255)                                           cGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCa                                                                           <  1:555760/140‑1 (MQ=255)                                                ccAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAAc                                                                      <  2:1152624/140‑1 (MQ=255)                                                ccAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAAc                                                                      <  1:35466/140‑1 (MQ=255)                                                ccAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAAc                                                                      <  2:442266/140‑1 (MQ=255)                                                                   gcgcACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACcgc                                                        >  2:148760/1‑135 (MQ=255)                                                                   gcgcACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACcgc                                                        <  1:148760/135‑1 (MQ=255)                                                                        caGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAGCGAAACCCGAACCGCGCAGGCGt                                                >  2:955258/1‑140 (MQ=255)                                                                           agCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATg                                           >  2:968106/1‑140 (MQ=255)                                                                             cAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCg                                         <  2:1229339/140‑1 (MQ=255)                                                                                   cataacaCCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGcc                                   >  2:739965/1‑140 (MQ=255)                                                                                     taacaCCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCaa                                 >  1:27331/1‑140 (MQ=255)                                                                                     taacaCCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCaa                                 >  2:946068/1‑140 (MQ=255)                                                                                     taacaCCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCaa                                 >  1:641480/1‑140 (MQ=255)                                                                                       acacCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAg                               >  2:316051/1‑140 (MQ=255)                                                                                         acCAAGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCt                             <  2:641480/140‑1 (MQ=255)                                                                                            aaGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCg                          >  2:521339/1‑140 (MQ=255)                                                                                             aGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCt                             >  1:1068608/1‑136 (MQ=255)                                                                                             aGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCt                             <  2:1068608/136‑1 (MQ=255)                                                                                             aGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTcc                           >  2:192827/1‑138 (MQ=255)                                                                                             aGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTcc                           <  1:192827/138‑1 (MQ=255)                                                                                             aGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCgg                         >  2:161949/1‑140 (MQ=255)                                                                                             aGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCgg                         >  2:349454/1‑140 (MQ=255)                                                                                             aGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCgg                         >  2:44656/1‑140 (MQ=255)                                                                                             aGTACCATAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCgg                         >  2:557026/1‑140 (MQ=255)                                                                                                    tAAACACCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATaa                  >  2:535920/1‑140 (MQ=255)                                                                                                         acCGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAAccgc             >  2:1223088/1‑140 (MQ=255)                                                                                                           cGTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAAccgccg           >  2:899837/1‑140 (MQ=255)                                                                                                            gTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGa          >  2:359359/1‑140 (MQ=255)                                                                                                            gTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGa          >  2:860824/1‑140 (MQ=255)                                                                                                            gTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGa          >  1:299607/1‑140 (MQ=255)                                                                                                            gTGATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGa          >  1:173564/1‑140 (MQ=255)                                                                                                              gATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGAcg        >  2:1173542/1‑140 (MQ=255)                                                                                                              gATCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGAcg        <  1:739965/140‑1 (MQ=255)                                                                                                               aTCGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGAcgc       >  2:471558/1‑140 (MQ=255)                                                                                                                 cGA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGa     <  1:349454/140‑1 (MQ=255)                                                                                                                  gA‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGaa    <  1:316051/140‑1 (MQ=255)                                                                                                                   a‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAg   >  2:955974/1‑140 (MQ=255)                                                                                                                   a‑‑CCCATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAg   >  2:113637/1‑140 (MQ=255)                                                                                                                    cccccATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAGCGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGaa    <  1:955258/138‑1 (MQ=255)                                                                                                                      cccATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGc  <  1:219861/140‑1 (MQ=255)                                                                                                                      cccATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGc  <  2:933575/140‑1 (MQ=255)                                                                                                                      cccATAACGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGc  <  1:1199860/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                     AATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGA‑‑CCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGC  >  NC_000913/1852623‑1852877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑