Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,950,998	T→C	V56A (GTT→GCT)	yecD →	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,950,998	0	T	C	100.0%	187.6 / NA	53	V56A (GTT→GCT)	yecD	putative hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/26);  total (27/26)

GATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAA  >  NC_000913/1950870‑1951134                                                                                                                                 |                                                                                                                                         gATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTg                                                                                                                               >  2:349249/1‑140 (MQ=255)     gATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGa                                                                                                                           <  1:785410/140‑1 (MQ=255)     gATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGa                                                                                                                           <  2:1227065/140‑1 (MQ=255)      aTTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGAt                                                                                                                          <  1:603394/140‑1 (MQ=255)      aTTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGAt                                                                                                                          <  2:188458/140‑1 (MQ=255)             agaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTg                                                                                                                   <  2:326869/140‑1 (MQ=255)              gaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGTCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGa                                                                                                                  <  1:924277/140‑1 (MQ=255)              gaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTTTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGa                                                                                                                  >  1:1010190/1‑140 (MQ=255)              gaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGa                                                                                                                  >  1:226319/1‑140 (MQ=255)                 ggCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGc                                                                                                               >  2:30372/1‑140 (MQ=255)                           cTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTTTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAg                                                                                                     <  2:1010190/140‑1 (MQ=255)                           cTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAg                                                                                                     <  2:226319/140‑1 (MQ=255)                                gCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGt                                                                                                >  2:530377/1‑140 (MQ=255)                                gCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGt                                                                                                >  1:867555/1‑140 (MQ=255)                                     tggtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATg                                                                                           >  1:1213946/1‑140 (MQ=255)                                         ccACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCC‑‑GTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCt                                                                                     >  2:525123/1‑140 (MQ=255)                                              tACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTATGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCAc                                                                                  <  1:972527/140‑1 (MQ=255)                                              tACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCAc                                                                                  <  1:832907/140‑1 (MQ=255)                                                 tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   >  2:796537/1‑136 (MQ=255)                                                 tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   >  2:21201/1‑136 (MQ=255)                                                 tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   <  1:21201/136‑1 (MQ=255)                                                 tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   >  2:608673/1‑136 (MQ=255)                                                 tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   <  1:608673/136‑1 (MQ=255)                                                 tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   <  1:796537/136‑1 (MQ=255)                                                 tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCgg                                                                               >  2:1130494/1‑140 (MQ=255)                                                 tGCCGATGAGGGAGTTAATCGCGCCGGGGAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCgg                                                                               >  2:1089174/1‑140 (MQ=255)                                                  gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   >  1:33123/1‑135 (MQ=255)                                                  gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   <  2:33123/135‑1 (MQ=255)                                                  gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGc                                                                              >  1:848683/1‑140 (MQ=255)                                                  gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGc                                                                              >  2:599026/1‑140 (MQ=255)                                                   ccGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCt                                                                             >  2:957161/1‑140 (MQ=255)                                                    cGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTa                                                                            >  1:26706/1‑140 (MQ=255)                                                    cGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTa                                                                            <  1:30372/140‑1 (MQ=255)                                                            tAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCC‑‑GTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGcc                                                                  <  1:525123/140‑1 (MQ=255)                                                             aGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGc                                                                   >  1:1142458/1‑140 (MQ=255)                                                                    cgcgCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGaaaa                                                            >  1:266503/1‑140 (MQ=255)                                                                     gcgcCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAAt                                                           >  2:302520/1‑140 (MQ=255)                                                                      cgcCGGGAAGCTGGCGGTGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAAtt                                                          >  1:241525/1‑140 (MQ=255)                                                                        ccGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtgg                                                        >  2:168739/1‑140 (MQ=255)                                                                        ccGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtgg                                                        >  1:463785/1‑140 (MQ=255)                                                                                cTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACa                                                <  1:349249/140‑1 (MQ=255)                                                                                 tGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACAt                                               <  1:1130494/140‑1 (MQ=255)                                                                                  ggcggcGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATc                                              <  2:26706/140‑1 (MQ=255)                                                                                     ggcgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCctg                                           <  1:599026/140‑1 (MQ=255)                                                                                     ggcgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCctg                                           <  2:867555/140‑1 (MQ=255)                                                                                       cgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCctgct                                         <  1:302520/140‑1 (MQ=255)                                                                                         aaaTTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtgg                                                        <  1:566982/123‑1 (MQ=255)                                                                                         aaaTTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtgg                                                        >  2:566982/1‑123 (MQ=255)                                                                                             ttCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTa                                   >  1:1259593/1‑140 (MQ=255)                                                                                              tCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAg                                  <  2:1142458/140‑1 (MQ=255)                                                                                                   ccAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCa                             <  2:848683/140‑1 (MQ=255)                                                                                                           cAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAg                     <  1:530377/140‑1 (MQ=255)                                                                                                                 gtgtTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGatat               >  1:1057952/1‑140 (MQ=255)                                                                                                                            gcgcgcTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAAtcatca    <  2:463785/140‑1 (MQ=255)                                                                                                                            gcgcgcTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAAtcatca    >  2:1111544/1‑140 (MQ=255)                                                                                                                             cgcgCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCaa   >  2:287722/1‑140 (MQ=255)                                                                                                                              gcgcTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCaaa  <  2:1213946/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                         GATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAA  >  NC_000913/1950870‑1951134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑