Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	163,050	T→C	S316P (TCC→CCC)	hrpB →	putative ATP‑dependent RNA helicase HrpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	163,050	0	T	C	100.0%	160.5 / NA	46	S316P (TCC→CCC)	hrpB	putative ATP‑dependent RNA helicase HrpB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/18);  total (28/18)

GACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGC  >  NC_000913/162917‑163185                                                                                                                                      |                                                                                                                                        gACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGa                                                                                                                                   >  2:82093/1‑140 (MQ=255)    cAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGc                                                                                                                                >  1:1001734/1‑140 (MQ=255)             tGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCg                                                                                                                       <  2:371165/140‑1 (MQ=255)                 aCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGgcg                                                                                                                   >  2:291462/1‑140 (MQ=255)                 aCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGgcg                                                                                                                   >  1:166762/1‑140 (MQ=255)                      tttAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCg                                                                                                              >  1:343979/1‑140 (MQ=255)                      tttAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCg                                                                                                              >  1:563104/1‑140 (MQ=255)                       ttAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCgg                                                                                                             <  1:291462/140‑1 (MQ=255)                              ttGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCgg                                                                                                             <  2:842388/133‑1 (MQ=255)                              ttGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCgg                                                                                                             >  1:842388/1‑133 (MQ=255)                                   ggTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGcc                                                                                                 <  1:987343/140‑1 (MQ=255)                                       ttCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGt                                                                                             >  2:678436/1‑140 (MQ=255)                                        tCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTa                                                                                            >  1:1141641/1‑140 (MQ=255)                                           tCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCa                                                                                         >  1:568436/1‑140 (MQ=255)                                             tggtggtggATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGc                                                                                       <  1:474698/140‑1 (MQ=255)                                             tggtggtggATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGc                                                                                       <  1:1242060/140‑1 (MQ=255)                                             tggtggtggATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGc                                                                                       >  1:1216077/1‑140 (MQ=255)                                              ggtggtggATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGcc                                                                                      >  1:328581/1‑140 (MQ=255)                                                  gtggATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCa                                                                                  >  2:728123/1‑140 (MQ=255)                                                        tgtgCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAAt                                                                            <  1:678436/140‑1 (MQ=255)                                                        tgtgCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAAt                                                                            <  2:444560/140‑1 (MQ=255)                                                         gtgCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATc                                                                           >  1:262213/1‑140 (MQ=255)                                                             ccAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCa                                                                       >  2:374078/1‑140 (MQ=255)                                                              cAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCaa                                                                      <  1:728123/140‑1 (MQ=255)                                                                 gAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGa                                                                   >  2:855461/1‑140 (MQ=255)                                                                 gAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGa                                                                   >  2:184174/1‑140 (MQ=255)                                                                 gAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGa                                                                   >  2:81836/1‑140 (MQ=255)                                                                 gAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGACACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGa                                                                   >  2:700077/1‑140 (MQ=255)                                                                      tgtgGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAg                                                              >  1:593339/1‑140 (MQ=255)                                                                      tgtgGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCATCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAg                                                              >  2:120025/1‑140 (MQ=255)                                                                                           cACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCaaa                                         <  2:568436/140‑1 (MQ=255)                                                                                             cGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGt                                       <  2:262213/140‑1 (MQ=255)                                                                                                   ttACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCg                                 <  2:675657/140‑1 (MQ=255)                                                                                                    tACGCGACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCgg                                <  1:81836/140‑1 (MQ=255)                                                                                                      cgcgACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGGAGAACGCGCCGCGGCGCAAAGTGAACCGgag                              >  1:134454/1‑140 (MQ=255)                                                                                                      cgcgACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGgag                              <  2:1216077/140‑1 (MQ=255)                                                                                                      cgcgACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGgag                              >  1:487744/1‑140 (MQ=255)                                                                                                      cgcgACTGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCATCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGgag                              <  1:120025/140‑1 (MQ=255)                                                                                                            tGATTACTCAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTa                        >  2:1261900/1‑140 (MQ=255)                                                                                                                    cAACGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGa                <  2:328581/140‑1 (MQ=255)                                                                                                                     aaCGCGTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGAt               >  2:483437/1‑140 (MQ=255)                                                                                                                       cgcgTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCt             >  1:755880/1‑140 (MQ=255)                                                                                                                        gcgTTAGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCtt            >  2:489793/1‑140 (MQ=255)                                                                                                                             aGCCAGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCgg       <  2:1141641/140‑1 (MQ=255)                                                                                                                                 aGGCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTg   <  1:483437/140‑1 (MQ=255)                                                                                                                                  ggCACCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGc  >  2:699935/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        GACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGC  >  NC_000913/162917‑163185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑