Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,016,495	G→A	A210T (GCC→ACC)	fliH →	flagellar biosynthesis protein FliH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,016,495	0	G	A	100.0%	260.0 / NA	74	A210T (GCC→ACC)	fliH	flagellar biosynthesis protein FliH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/41);  total (33/41)

GCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGCCTGCGGTAC  >  NC_000913/2016358‑2016629                                                                                                                                          |                                                                                                                                       gcgTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACAcc                                                                                                                                      >  2:493979/1‑140 (MQ=255) gcgTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTAAAGTTTGCATGGCTTGCGCTGGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACAcc                                                                                                                                      <  2:778636/140‑1 (MQ=255)  cgTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCa                                                                                                                                     >  2:682605/1‑140 (MQ=255)             atgatCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACt                                                                                                                          >  2:335778/1‑140 (MQ=255)               gatCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCg                                                                                                                        <  1:312510/140‑1 (MQ=255)               gatCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCg                                                                                                                        <  2:566376/140‑1 (MQ=255)                    gCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGc                                                                                                                   <  1:719868/140‑1 (MQ=255)                    gCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGc                                                                                                                   <  2:422533/140‑1 (MQ=255)                    gCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGc                                                                                                                   <  1:133187/140‑1 (MQ=255)                             ggATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAActct                                                                                                          >  1:760812/1‑140 (MQ=255)                              gatgatATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTg                                                                                                         <  2:184620/140‑1 (MQ=255)                                 gatATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCg                                                                                                      >  1:520140/1‑140 (MQ=255)                                    atGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCt                                                                                                   >  1:280170/1‑140 (MQ=255)                                     tGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTg                                                                                                  <  2:843834/140‑1 (MQ=255)                                      gCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTgg                                                                                                 >  1:1075690/1‑140 (MQ=255)                                       cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                                                                <  1:682605/140‑1 (MQ=255)                                       cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                                                                <  1:132814/140‑1 (MQ=255)                                       cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                                                                <  2:810931/140‑1 (MQ=255)                                       cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                                                                <  1:142266/140‑1 (MQ=255)                                       cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                                                                <  1:340922/140‑1 (MQ=255)                                             gcTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCAcc                                                                                          >  1:59510/1‑140 (MQ=255)                                               tACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAg                                                                                        <  2:59510/140‑1 (MQ=255)                                               tACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAg                                                                                        <  1:335778/140‑1 (MQ=255)                                                 ccTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGa                                                                                      <  2:858419/140‑1 (MQ=255)                                                 ccTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGa                                                                                      >  2:284005/1‑140 (MQ=255)                                                 ccTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGa                                                                                      >  1:110185/1‑140 (MQ=255)                                                  cTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAg                                                                                     <  2:520140/140‑1 (MQ=255)                                                   ttAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAgt                                                                                    <  2:411466/140‑1 (MQ=255)                                                    tAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAgtg                                                                                   <  2:760812/140‑1 (MQ=255)                                                           gCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTa                                                                              >  2:111655/1‑138 (MQ=255)                                                           gCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTa                                                                              <  1:111655/138‑1 (MQ=255)                                                           gCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAAt                                                                            >  2:340307/1‑140 (MQ=255)                                                           gCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAAt                                                                            >  1:846762/1‑140 (MQ=255)                                                           gCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAAt                                                                            >  1:256754/1‑140 (MQ=255)                                                            cATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCAcc                                                                                          >  1:205646/1‑125 (MQ=255)                                                            cATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCAcc                                                                                          <  2:205646/125‑1 (MQ=255)                                                                gctggcGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCa                                                                       <  2:645148/140‑1 (MQ=255)                                                                gctggcGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCa                                                                       <  2:846762/140‑1 (MQ=255)                                                                         tGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGa                                                              >  1:566644/1‑140 (MQ=255)                                                                           cGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACt                                                            >  1:700287/1‑140 (MQ=255)                                                                            ggggCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTc                                                           <  1:493979/140‑1 (MQ=255)                                                                              ggCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGc                                                         <  1:147633/140‑1 (MQ=255)                                                                                   tCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCt                                                    <  2:256754/140‑1 (MQ=255)                                                                                        cccTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCa                                               <  1:1106502/140‑1 (MQ=255)                                                                                          cTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             >  1:533217/1‑140 (MQ=255)                                                                                          cTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             <  1:284005/140‑1 (MQ=255)                                                                                             cATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             <  1:1136993/137‑1 (MQ=255)                                                                                             cATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             >  2:1136993/1‑137 (MQ=255)                                                                                             cATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTg                                          >  2:1052800/1‑140 (MQ=255)                                                                                             cATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTg                                          >  1:1016523/1‑140 (MQ=255)                                                                                             cATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTg                                          >  2:668977/1‑140 (MQ=255)                                                                                             cATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTg                                          >  1:123046/1‑140 (MQ=255)                                                                                              aTCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             <  1:856325/136‑1 (MQ=255)                                                                                              aTCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             >  2:856325/1‑136 (MQ=255)                                                                                               tCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             <  2:734628/135‑1 (MQ=255)                                                                                               tCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             >  1:734628/1‑135 (MQ=255)                                                                                                   ggcggcTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             <  2:1182072/131‑1 (MQ=255)                                                                                                   ggcggcTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             >  1:1182072/1‑131 (MQ=255)                                                                                                    gcggcTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACNAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             >  2:686937/1‑130 (MQ=255)                                                                                                    gcggcTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             <  1:686937/130‑1 (MQ=255)                                                                                                      ggcTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACttt                                 <  2:123046/140‑1 (MQ=255)                                                                                                         tGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGaa                              >  2:459961/1‑140 (MQ=255)                                                                                                              aGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCaa                         <  1:1052800/140‑1 (MQ=255)                                                                                                                  tcCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATg                     >  1:171021/1‑140 (MQ=255)                                                                                                                  tcCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATg                     <  2:700287/140‑1 (MQ=255)                                                                                                                  tcCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATg                     >  1:454087/1‑140 (MQ=255)                                                                                                                    cgccgATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGgc                   >  2:590795/1‑140 (MQ=255)                                                                                                                     gccgATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGgcg                  >  2:508071/1‑140 (MQ=255)                                                                                                                         aTGAAGGCGATCTCGACACCAGTGTCGCCACTCGTTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGt              >  2:1230056/1‑140 (MQ=255)                                                                                                                          tGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGtt             <  2:171021/140‑1 (MQ=255)                                                                                                                            aaGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGc           <  1:508071/140‑1 (MQ=255)                                                                                                                               gCGATCTCGACACCAGTGTCGCCACTCGTTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGCCTg        <  1:1230056/140‑1 (MQ=255)                                                                                                                               gCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGCCTg        <  1:590795/140‑1 (MQ=255)                                                                                                                                     tcGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGCCTGCGGTAc  <  1:1140067/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       GCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGCCTGCGGTAC  >  NC_000913/2016358‑2016629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑