Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,097,752	T→C	T184A (ACG→GCG)	wzzB ←	regulator of length of O‑antigen component of lipopolysaccharide chains

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,097,752	0	T	C	100.0%	301.1 / NA	85	T184A (ACG→GCG)	wzzB	regulator of length of O‑antigen component of lipopolysaccharide chains
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/45);  total (40/45)

ACGTATCTTGTGTGATATCTTCGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGTTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTGCGCCCTCTGCAGTTTG  >  NC_000913/2097617‑2097887                                                                                                                                        |                                                                                                                                        acGTATCTTGTGTGATATCTTCGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCtt                                                                                                                                     >  2:773510/1‑140 (MQ=255)  cGTATCTTGTGTGATATCTTCGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTa                                                                                                                                    <  2:137625/140‑1 (MQ=255)   gTATCTTGTGTGATATCTTCGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTaa                                                                                                                                   >  1:90604/1‑140 (MQ=255)           tgtgATATCTTCGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCt                                                                                                                           >  1:391514/1‑140 (MQ=255)           tgtgATATCTTCGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCt                                                                                                                           <  2:518027/140‑1 (MQ=255)            gtgATATCTTCGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTg                                                                                                                          <  2:90604/140‑1 (MQ=255)             tgATATCTTCGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGc                                                                                                                         >  1:856383/1‑140 (MQ=255)                tatCTTCGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAg                                                                                                                      >  1:1190281/1‑140 (MQ=255)                 atCTTCGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGt                                                                                                                     >  2:1104918/1‑140 (MQ=255)                  tCTTCGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGtt                                                                                                                    >  2:214667/1‑140 (MQ=255)                    ttCGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGtttt                                                                                                                  >  1:316571/1‑140 (MQ=255)                      cGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTc                                                                                                                <  1:1237532/140‑1 (MQ=255)                      cGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTc                                                                                                                <  2:477662/140‑1 (MQ=255)                      cGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTc                                                                                                                <  1:384252/140‑1 (MQ=255)                       gCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTcc                                                                                                               >  2:321216/1‑140 (MQ=255)                        ccAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCg                                                                                                              <  1:1050164/140‑1 (MQ=255)                           gTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTcc                                                                                                           <  1:473810/140‑1 (MQ=255)                           gTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTcc                                                                                                           <  2:440179/140‑1 (MQ=255)                            tCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTccc                                                                                                          >  1:33655/1‑140 (MQ=255)                                ttGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTc                                                                                                                <  2:283069/130‑1 (MQ=255)                                ttGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTc                                                                                                                >  1:283069/1‑130 (MQ=255)                                   aaTCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCagag                                                                                                      <  1:336302/137‑1 (MQ=255)                                   aaTCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCagag                                                                                                      >  2:336302/1‑137 (MQ=255)                                   aaTCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCaa                                                                                                   <  1:1015943/140‑1 (MQ=255)                                     tCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAAtg                                                                                                 <  1:214667/140‑1 (MQ=255)                                     tCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAAtg                                                                                                 <  2:1190281/140‑1 (MQ=255)                                     tCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAAtg                                                                                                 <  1:1104918/140‑1 (MQ=255)                                      cTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAAtgt                                                                                                <  2:430076/140‑1 (MQ=255)                                      cTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAAtgt                                                                                                >  1:58665/1‑140 (MQ=255)                                      cTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAAtgt                                                                                                <  1:773510/140‑1 (MQ=255)                                        gCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAAtgttg                                                                                              <  1:250057/140‑1 (MQ=255)                                         cGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAAtgttgt                                                                                             >  1:234867/1‑140 (MQ=255)                                                   aCCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATc                                                                                   <  1:632778/140‑1 (MQ=255)                                                   aCCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATc                                                                                   >  1:650237/1‑140 (MQ=255)                                                      tGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCtt                                                                                >  1:254425/1‑140 (MQ=255)                                                      tGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCtt                                                                                >  2:558256/1‑140 (MQ=255)                                                        cgcCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCtttt                                                                              >  2:659691/1‑140 (MQ=255)                                                        cgcCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCtttt                                                                              >  2:81477/1‑140 (MQ=255)                                                        cgcCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCtttt                                                                              >  1:804499/1‑140 (MQ=255)                                                           cTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTa                                                                           <  1:321216/140‑1 (MQ=255)                                                           cTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTa                                                                           <  1:456631/140‑1 (MQ=255)                                                           cTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTa                                                                           <  1:798419/140‑1 (MQ=255)                                                              aTTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTg                                                                                        <  2:926375/124‑1 (MQ=255)                                                              aTTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTg                                                                                        >  1:926375/1‑124 (MQ=255)                                                               ttCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGctc                                                                       <  1:366495/140‑1 (MQ=255)                                                                tCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGctct                                                                      <  1:657013/140‑1 (MQ=255)                                                                   cATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGa                                                                   <  2:322684/140‑1 (MQ=255)                                                                    aTACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGAt                                                                  <  2:254425/140‑1 (MQ=255)                                                                     tACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGAtt                                                                 <  1:1104315/140‑1 (MQ=255)                                                                          cAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACtt                                                            >  1:111013/1‑140 (MQ=255)                                                                          cAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACtt                                                            >  1:176075/1‑140 (MQ=255)                                                                            aCGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTa                                                          <  1:659691/140‑1 (MQ=255)                                                                               cTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCg                                                       <  2:58665/140‑1 (MQ=255)                                                                                  ccTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCa                                                    >  1:392067/1‑140 (MQ=255)                                                                                  ccTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCa                                                    >  1:1238380/1‑140 (MQ=255)                                                                                  ccTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCa                                                    >  2:259319/1‑140 (MQ=255)                                                                                  ccTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTCCTAGCTCTTGATTCACTTTATCGTCa                                                    >  1:604009/1‑140 (MQ=255)                                                                                   cTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCaa                                                   <  1:546844/140‑1 (MQ=255)                                                                                       aTCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTg                                               <  2:176075/140‑1 (MQ=255)                                                                                            aCGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGaa                                          >  2:1225808/1‑140 (MQ=255)                                                                                            aCGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGaa                                          <  1:259319/140‑1 (MQ=255)                                                                                                aTACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTa                                      >  1:929480/1‑140 (MQ=255)                                                                                                aTACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTa                                      <  1:1225808/140‑1 (MQ=255)                                                                                                aTACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTa                                      >  1:1014816/1‑140 (MQ=255)                                                                                                aTACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGCCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAAc                                                  >  1:153656/1‑128 (MQ=255)                                                                                                aTACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGCCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAAc                                                  <  2:153656/128‑1 (MQ=255)                                                                                                       gATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGcc                               <  2:111013/140‑1 (MQ=255)                                                                                                       gATCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGcc                               <  1:81477/140‑1 (MQ=255)                                                                                                        aTCTTTCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTg                                               <  1:892459/123‑1 (MQ=255)                                                                                                        aTCTTTCTGCTCCTGCGCGACGACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTg                                               >  2:892459/1‑123 (MQ=255)                                                                                                            ttCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACtt                          <  2:392067/140‑1 (MQ=255)                                                                                                             tCTGCTCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTc                         >  1:244693/1‑140 (MQ=255)                                                                                                                  tCCTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTtg                    >  2:839582/1‑140 (MQ=255)                                                                                                                   ccTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTtgt                   <  1:521250/140‑1 (MQ=255)                                                                                                                    cTGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTtgtg                  >  1:457245/1‑140 (MQ=255)                                                                                                                     tGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTgc                 <  1:827600/140‑1 (MQ=255)                                                                                                                     tGCGCGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTgc                 <  2:1217475/140‑1 (MQ=255)                                                                                                                      gcgcGACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTgcg                >  1:363124/1‑140 (MQ=255)                                                                                                                       cgcgACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTgcgc               >  1:894059/1‑140 (MQ=255)                                                                                                                       cgcgACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTgc                 >  2:606680/1‑138 (MQ=255)                                                                                                                       cgcgACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTgc                 <  1:606680/138‑1 (MQ=255)                                                                                                                        gcgACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTGCGcc              <  1:849027/140‑1 (MQ=255)                                                                                                                        gcgACTACTTCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTGCGcc              <  1:505166/140‑1 (MQ=255)                                                                                                                                 tCCTGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTGCGCCCTCTGCAGt     <  2:804499/140‑1 (MQ=255)                                                                                                                                    tGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTGCGCCCTCTGCAGTTTg  >  2:488249/1‑140 (MQ=255)                                                                                                                                    tGCGCTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTGCGCCCTCTGCAGTTTg  >  2:1151842/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        ACGTATCTTGTGTGATATCTTCGCCAGTCTGTTGAATCTGCGGTTTTGTCACCTGCGCCTGATTCGCATACTGCAACGCTTCCTGAATCTGACGGATACGCAGATCTTTCTGCTCCTGCGCGACTACTTCCTGCGTTCTTAAAGAGTCCTGCAAGTTTTTCCGTCCCAGAGCAATGTTGTCTTTGAGATCCTTTTCTAGCTCTTGATTCACTTTATCGTCAACTTGCTGAATGTATTGGGCCAACTTCATTTGTGCGCCCTCTGCAGTTTG  >  NC_000913/2097617‑2097887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑