Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,119,684	T→C	T159A (ACG→GCG)	wzxC ←	M‑antigen undecaprenyl disphosphate flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,119,684	0	T	C	100.0%	128.2 / NA	39	T159A (ACG→GCG)	wzxC	M‑antigen undecaprenyl disphosphate flippase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/16);  total (23/16)

GCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAACAGCAGCGTTCTCACCGCACTATTGACCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGTACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCCCGTGGGGGATTACCACAAACGCCAGCGATAATGTTTTAAT  >  NC_000913/2119548‑2119816                                                                                                                                         |                                                                                                                                     gCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAACAGCAGCGTTCTCACCGCACTATTGACCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGcaca                                                                                                                                   >  2:992003/1‑140 (MQ=255)        agaAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAACAGCAGCGTTCTCACCGCACTATTGACCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAg                                                                                                                            <  2:1048284/140‑1 (MQ=255)                 gACCGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAACAGCAGCGTTCTCACCGCACTATTGACCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGc                                                                                                                   >  1:243348/1‑140 (MQ=255)                 gACCGGGGCGATAAATTTCGCGGCCAAAGTAGCCAAACAGCAGCGTTCTCACCGCACTATTGACCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGc                                                                                                                   >  1:941565/1‑140 (MQ=255)                   ccGGGGGGATAAATTTTGCGGCAAAAGTAGCCAAACAGCACCGTTCTCACCGCCCTATTGACCAGATAACCGGGGACCGCGGTCATCGCCAGCGGCGAGAAAGGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCAc                                                                                                                 <  2:992374/140‑1 (MQ=255)                    cGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAACAGCAGCGTTCTCACCGCACTATTGACCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCAcc                                                                                                                >  2:1067209/1‑140 (MQ=255)                       ggCGATAAATTTTGCGGCCAAAGTAGCCAAACAGCAGCGTTCTCACCGCACTATTGACCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACcgcg                                                                                                             <  2:243348/140‑1 (MQ=255)                       ggCGATAAATTTCGCGGCCAAAGTAGCCAAACAGCAGCGTTCTCACCGCACTATTGACCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACcgcg                                                                                                             <  2:941565/140‑1 (MQ=255)                                 tttGCGGCCAAAGTAGCCAAACAGCAGCGTTCTCACCGCACTATTGACCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGa                                                                                                   >  2:79552/1‑140 (MQ=255)                                  ttGCGGCCAAAGTAGCCAAACAGCAGCGTTCTCACCGCACTATTGACCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGAt                                                                                                  <  1:992003/140‑1 (MQ=255)                                                  cAAACAGCAGCGTTCTCACCGCACTATTGACCAGATAACCGAGGATCGCGGTCACCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTttgttg                                                                                  <  2:646235/140‑1 (MQ=255)                                                            cGTTCTCACCGCACTATTGACCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGct                                                                        >  2:21906/1‑140 (MQ=255)                                                                        aCTATTGACCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCCCCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCa                                                            <  1:158282/140‑1 (MQ=255)                                                                        aCTATTGACCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCa                                                            <  1:437330/140‑1 (MQ=255)                                                                               aCCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAAcgcgcg                                                     >  2:154313/1‑140 (MQ=255)                                                                               aCCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAAcgcgcg                                                     >  1:1245473/1‑140 (MQ=255)                                                                                    aTAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAAcg                                                         <  2:1216209/131‑1 (MQ=255)                                                                                    aTAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAAcg                                                         >  1:1216209/1‑131 (MQ=255)                                                                                    aTAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACt                                                >  2:713933/1‑140 (MQ=255)                                                                                    aTAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACt                                                >  2:1041909/1‑140 (MQ=255)                                                                                    aTAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACt                                                >  1:670824/1‑140 (MQ=255)                                                                                      aaCCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCa                                                            >  2:209871/1‑126 (MQ=255)                                                                                      aaCCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCa                                                            <  1:209871/126‑1 (MQ=255)                                                                                      aaCCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGt                                              >  1:956373/1‑140 (MQ=255)                                                                                      aaCCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGt                                              >  1:774688/1‑140 (MQ=255)                                                                                      aaCCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGt                                              >  2:320849/1‑140 (MQ=255)                                                                                       aCCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGtt                                             <  1:713933/140‑1 (MQ=255)                                                                                          gAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGcc                                          <  1:1067209/140‑1 (MQ=255)                                                                                            ggATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCCCg                                        <  1:1041909/140‑1 (MQ=255)                                                                                             gATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCCCGt                                       >  1:561568/1‑140 (MQ=255)                                                                                              aTCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCCCGTg                                      <  1:79552/140‑1 (MQ=255)                                                                                              aTCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCCCGTg                                      >  1:1019824/1‑140 (MQ=255)                                                                                                  cgGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCCCGTggggg                                  >  2:80959/1‑140 (MQ=255)                                                                                                    gTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCACGTGGGGGAt                                <  1:1108109/140‑1 (MQ=255)                                                                                                      cATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCCCGTGGGGGATTa                              >  2:30190/1‑140 (MQ=255)                                                                                                       aTCGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCCCGTGGGGGATTAc                             >  1:708500/1‑140 (MQ=255)                                                                                                         cGCCAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCCCGTGGGGGATTACca                           >  2:802777/1‑140 (MQ=255)                                                                                                           ccAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCCCATGGGGGATTACcaca                         >  2:779757/1‑140 (MQ=255)                                                                                                            cAGCGGCCAGAAATGGGCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCCCGTGGGGGATTACCACaa                        <  2:419638/140‑1 (MQ=255)                                                                                                                           ggCGCTAACCACCGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCCCGTGGGGGATTACCACAAACGCCAGCGATAATg         >  1:262357/1‑140 (MQ=255)                                                                                                                                  accaccGCACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCCCGTGGGGGATTACCACAAACGCCAGCGATAATGTTTTAAt  <  2:708500/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     GCCAGCGAGAAATGCAGACCGGGGCGATAAATTTTGCGGCCAAAGTAGCCAAACAGCAGCGTTCTCACCGCACTATTGACCAGATAACCGAGGATCGCGGTCATCGCCAGCGGCCAGAAATGGGCGCTAACCACCGTACAAGTGAAGCCCGCCAGCACCGCGCTGGTTTCGATCATGCCGATTTTGTTGAACTCCAGCTCTTTTTGCATCAACGCGCGGAACTGTTGCCCGTGGGGGATTACCACAAACGCCAGCGATAATGTTTTAAT  >  NC_000913/2119548‑2119816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑