Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,253,116	T→C	T344A (ACG→GCG)	nupX ←	putative nucleoside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,253,116	0	T	C	100.0%	208.6 / NA	59	T344A (ACG→GCG)	nupX	putative nucleoside transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/34);  total (25/34)

GGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAG  >  NC_000913/2252982‑2253245                                                                                                                                       |                                                                                                                                  ggCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCa                                                                                                                              >  1:896081/1‑140 (MQ=255) ggCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCa                                                                                                                              >  1:121334/1‑140 (MQ=255)   cGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGa                                                                                                                            <  2:375007/140‑1 (MQ=255)   cGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGa                                                                                                                            <  1:396466/140‑1 (MQ=255)   cGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGa                                                                                                                            <  2:66565/140‑1 (MQ=255)   cGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGa                                                                                                                            <  2:687753/140‑1 (MQ=255)   cGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGa                                                                                                                            <  2:121334/140‑1 (MQ=255)   cGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGa                                                                                                                            <  1:1087552/140‑1 (MQ=255)     aTTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATa                                                                                                                          <  1:270965/140‑1 (MQ=255)       ttCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAgg                                                                                                                        <  1:594383/140‑1 (MQ=255)         ccGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAGGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGt                                                                                                                      <  2:1245461/140‑1 (MQ=255)          cGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTg                                                                                                                     <  1:1144322/140‑1 (MQ=255)            gcgcACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTgag                                                                                                                   <  1:1168396/140‑1 (MQ=255)                      tgGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGAt                                                                                                         >  1:1180548/1‑140 (MQ=255)                            aaCCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGa                                                                                                   <  2:395976/140‑1 (MQ=255)                              ccGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACa                                                                                                 >  1:139198/1‑140 (MQ=255)                              ccGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACa                                                                                                 >  2:759990/1‑140 (MQ=255)                              ccGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACa                                                                                                 >  2:254383/1‑140 (MQ=255)                                    aaaaCGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCa                                                                                           <  1:633331/140‑1 (MQ=255)                                      aaCGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCAtt                                                                                         >  1:963337/1‑140 (MQ=255)                                                 caccaccCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGtt                                                                              <  1:655701/140‑1 (MQ=255)                                                  accaccCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGttt                                                                             <  1:759990/140‑1 (MQ=255)                                                         cGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTg                                                                          <  2:792134/136‑1 (MQ=255)                                                         cGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTg                                                                          >  1:792134/1‑136 (MQ=255)                                                           aTAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGcc                                                                                  <  1:1079564/126‑1 (MQ=255)                                                           aTAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGcc                                                                                  >  2:1079564/1‑126 (MQ=255)                                                              gAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCaa                                                                 <  2:160838/140‑1 (MQ=255)                                                               aaCCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCaaa                                                                <  2:1180548/140‑1 (MQ=255)                                                                aCCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAAc                                                               >  1:327242/1‑140 (MQ=255)                                                                 ccAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTAAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACt                                                              >  1:1060183/1‑140 (MQ=255)                                                                    aaGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAACATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTccc                                                           >  1:686439/1‑140 (MQ=255)                                                                        tAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCa                                                       >  1:249328/1‑140 (MQ=255)                                                                         aGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGc                                                        >  1:407098/1‑138 (MQ=255)                                                                         aGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGc                                                        <  2:407098/138‑1 (MQ=255)                                                                             aaaCCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGAtt                                                  <  2:686439/140‑1 (MQ=255)                                                                             aaaCCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGAtt                                                  <  2:249328/140‑1 (MQ=255)                                                                               aCCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCg                                                <  1:1106301/140‑1 (MQ=255)                                                                               aCCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCg                                                >  2:275213/1‑140 (MQ=255)                                                                                   cacaACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGAt                                                   >  1:578792/1‑133 (MQ=255)                                                                                   cacaACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGAt                                                   <  2:578792/133‑1 (MQ=255)                                                                                   cacaACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATc                                            >  1:1219773/1‑140 (MQ=255)                                                                                    acaACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCa                                           <  2:17115/140‑1 (MQ=255)                                                                                       aCGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAGCTCCCGGCAAGATTCGCATCACTc                                        <  2:384100/140‑1 (MQ=255)                                                                                       aCGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTc                                        <  2:1189694/140‑1 (MQ=255)                                                                                       aCGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTc                                        <  2:1219773/140‑1 (MQ=255)                                                                                       aCGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTAAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTc                                        <  2:1060183/140‑1 (MQ=255)                                                                                        cgcgAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTcc                                       <  2:1242266/140‑1 (MQ=255)                                                                                           gAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGt                                    >  2:259866/1‑140 (MQ=255)                                                                                           gAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGt                                    >  1:142004/1‑140 (MQ=255)                                                                                                   taatCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACAccc                            >  2:305181/1‑140 (MQ=255)                                                                                                         ccACAGTTTTAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCAcc                      >  1:165371/1‑140 (MQ=255)                                                                                                                tttAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACgtca               <  2:47681/140‑4 (MQ=255)                                                                                                                tttAGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCa               <  1:275213/140‑1 (MQ=255)                                                                                                                   aGCATCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTg            <  1:305181/140‑1 (MQ=255)                                                                                                                       tCGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGc        >  2:78893/1‑140 (MQ=255)                                                                                                                        cGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGcc       <  2:921730/140‑1 (MQ=255)                                                                                                                        cGAGAGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCACGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGcc       >  2:306134/1‑140 (MQ=255)                                                                                                                         gagaGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCca      <  2:139198/140‑1 (MQ=255)                                                                                                                         gagaGTGCCAGCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCca      >  1:388521/1‑140 (MQ=255)                                                                                                                                   gCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCcagcag  <  2:578/134‑1 (MQ=255)                                                                                                                                   gCCGCTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCcagcag  >  1:578/1‑134 (MQ=255)                                                                                                                                       |                                                                                                                                  GGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAG  >  NC_000913/2252982‑2253245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑