Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,294,012	A→G	V298A (GTG→GCG)	ccmF ←	holocytochrome c synthetase membrane subunit CcmF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,294,012	0	A	G	100.0%	243.7 / NA	71	V298A (GTG→GCG)	ccmF	holocytochrome c synthetase membrane subunit CcmF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/36);  total (35/36)

TTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAGCGCAGTCCCCACC  >  NC_000913/2293880‑2294143                                                                                                                                     |                                                                                                                                      tttACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGa                                                                                                                                <  2:593864/140‑1 (MQ=255)  ttACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGaa                                                                                                                               <  2:760689/140‑1 (MQ=255)  ttACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGaa                                                                                                                               <  2:1205858/140‑1 (MQ=255)         tGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGaa                                                                                                                               <  1:1068472/133‑1 (MQ=255)         tGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGaa                                                                                                                               >  2:1068472/1‑133 (MQ=255)           aGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAgg                                                                                                                      >  2:746802/1‑140 (MQ=255)           aGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAgg                                                                                                                      >  2:589088/1‑140 (MQ=255)           aGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAgg                                                                                                                      >  2:224741/1‑140 (MQ=255)           aGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAgg                                                                                                                      >  2:1027544/1‑140 (MQ=255)           aGCGAACTTTATGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAgg                                                                                                                      >  1:37917/1‑140 (MQ=255)                  tttGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTcc                                                                                                               <  1:589088/140‑1 (MQ=255)                   ttGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTccc                                                                                                              >  2:191526/1‑140 (MQ=255)                    tgtgTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTcccc                                                                                                             <  1:777775/140‑1 (MQ=255)                    tgtgTCCACGCGCGGCAAACAGAAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTcccc                                                                                                             <  2:1019636/140‑1 (MQ=255)                     gtgtCCACGCGCGGCAAACAGCGGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCa                                                                                                            <  2:1119517/140‑1 (MQ=255)                          cACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGa                                                                                                       <  1:746802/140‑1 (MQ=255)                                   cAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACgag                                                                                              <  1:350792/140‑1 (MQ=255)                                       cagcagcagCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAgg                                                                                          <  1:224741/140‑1 (MQ=255)                                             cagcGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGa                                                                                    >  1:836223/1‑140 (MQ=255)                                               gcGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGAtt                                                                                  >  2:510000/1‑140 (MQ=255)                                               gcGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGAtt                                                                                  <  1:1027544/140‑1 (MQ=255)                                                  aaCCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGAtt                                                                                  >  1:74353/1‑137 (MQ=255)                                                  aaCCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGAtt                                                                                  <  2:74353/137‑1 (MQ=255)                                                    ccGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCca‑‑g                                                                           <  2:1018123/140‑1 (MQ=255)                                                        cAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGt                                                                       <  1:1044091/140‑1 (MQ=255)                                                                       tAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTg                                                        >  1:609025/1‑140 (MQ=255)                                                                       tAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTg                                                        >  2:988065/1‑140 (MQ=255)                                                                       tAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTg                                                        <  2:836223/140‑1 (MQ=255)                                                                            gCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCt                                                   >  1:1112710/1‑140 (MQ=255)                                                                               aGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGgc                                                >  2:901213/1‑140 (MQ=255)                                                                               aGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGgc                                                >  1:640118/1‑140 (MQ=255)                                                                               aGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGgc                                                <  2:37917/140‑1 (MQ=255)                                                                               aGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGgc                                                >  2:324213/1‑140 (MQ=255)                                                                               aGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGgc                                                >  2:201943/1‑140 (MQ=255)                                                                               aGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGgc                                                >  2:1116276/1‑140 (MQ=255)                                                                                 gATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGgcgc                                              >  1:1164551/1‑140 (MQ=255)                                                                                 gATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGgcgc                                              <  1:324213/140‑1 (MQ=255)                                                                                 gATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGgcgc                                              <  1:201943/140‑1 (MQ=255)                                                                                  aTAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGcgcg                                             <  2:609025/140‑1 (MQ=255)                                                                                  aTAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGcgcg                                             <  1:1116276/140‑1 (MQ=255)                                                                                         tACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCa                                      <  2:38419/140‑1 (MQ=255)                                                                                          aCCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAg                                     >  2:793499/1‑140 (MQ=255)                                                                                          aCCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAg                                     >  1:1059186/1‑140 (MQ=255)                                                                                           ccGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGt                                    >  1:950562/1‑140 (MQ=255)                                                                                           ccGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGt                                    >  1:966325/1‑140 (MQ=255)                                                                                            cgcgcgCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAg                                     >  2:658136/1‑140 (MQ=255)                                                                                            cgcgcgCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGcgcg                                             <  2:1154052/130‑1 (MQ=255)                                                                                            cgcgcgCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGcgcg                                             >  1:1154052/1‑130 (MQ=255)                                                                                            cgcgcgCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTg                                   >  1:1075471/1‑140 (MQ=255)                                                                                            cgcgcgCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCACCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGt                                    <  2:1735/139‑1 (MQ=255)                                                                                            cgcgcgCCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCACCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGt                                    >  1:1735/1‑139 (MQ=255)                                                                                             gcgcgcCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGa                                  <  2:1061171/140‑1 (MQ=255)                                                                                             gcgcgcCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGa                                  <  1:191526/140‑1 (MQ=255)                                                                                              cgcgcCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGAc                                 >  2:292466/1‑140 (MQ=255)                                                                                              cgcgcCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGAc                                 >  1:1005479/1‑140 (MQ=255)                                                                                                cgcCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCg                               >  1:4734/1‑140 (MQ=255)                                                                                                cgcCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCg                               <  2:19256/140‑1 (MQ=255)                                                                                                cgcCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCg                               >  1:399625/1‑140 (MQ=255)                                                                                                 gcCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGc                              <  1:988065/140‑1 (MQ=255)                                                                                                 gcCGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGc                              >  2:381609/1‑140 (MQ=255)                                                                                                   cGGATCAGACGCAACCGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCa                            <  2:399625/140‑1 (MQ=255)                                                                                                   cGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCa                            <  2:640118/140‑1 (MQ=255)                                                                                                   cGGATCAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCa                            <  1:381609/140‑1 (MQ=255)                                                                                                        cAGACGCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCagtgag                       <  1:793499/140‑1 (MQ=255)                                                                                                             gCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCAt                  <  2:532290/140‑1 (MQ=255)                                                                                                             gCAAACGCGTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCAt                  <  2:1075471/140‑1 (MQ=255)                                                                                                                  cgcgTGTACCGATCCCAGCGCGCCGGAACGCACGAGGAAAGCCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAgcg             <  2:1133176/140‑1 (MQ=255)                                                                                                                  cgcgTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAgcg             <  2:4734/140‑1 (MQ=255)                                                                                                                  cgcgTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGAACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAgcg             <  1:510000/140‑1 (MQ=255)                                                                                                                  cgcgTGTACCGATACCAGCGCGCCGGAACGCACGAGGAAAGCCCCCAACAGCCACAACAAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCCCGCTTTGAAGCGGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAgcg             <  2:359650/140‑1 (MQ=255)                                                                                                                             aTACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAgcg             <  2:796888/129‑1 (MQ=255)                                                                                                                             aTACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAgcg             >  1:796888/1‑129 (MQ=255)                                                                                                                             aTACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAGCGCAGTCCccacc  >  2:632304/1‑140 (MQ=255)                                                                                                                             aTACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAGCGCAGTCCccacc  <  2:1164551/140‑1 (MQ=255)                                                                                                                             aTACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAGCGCAGTCCccac   >  1:837909/1‑139 (MQ=255)                                                                                                                             aTACCAGCGCGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAGCGCAGTCCccac   <  2:837909/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      TTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCA‑‑GCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAGCGCAGTCCCCACC  >  NC_000913/2293880‑2294143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑