Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,306,567	T→C	E63G (GAG→GGG)	mqo ←	malate:quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,306,567	0	T	C	100.0%	177.0 / NA	51	E63G (GAG→GGG)	mqo	malate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/20);  total (31/20)

GGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCTCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCGCCATCGA  >  NC_000913/2306434‑2306700                                                                                                                                       |                                                                                                                                      ggCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCccacca                                                                                                                                 <  2:603515/140‑1 (MQ=255)      cTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTc                                                                                                                            <  2:504690/140‑1 (MQ=255)                 aTACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTc                                                                                                                 <  2:1122966/140‑1 (MQ=255)                       aTGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCCGTTCGAACTCTCCTGCGCGGCACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCt                                                                                                            >  2:666934/1‑140 (MQ=255)                       aTGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTc                                                                                                           >  2:300794/1‑140 (MQ=255)                       aTGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTc                                                                                                           >  2:1131654/1‑140 (MQ=255)                       aTGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTc                                                                                                           >  2:479663/1‑140 (MQ=255)                       aTGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTc                                                                                                           >  1:734098/1‑140 (MQ=255)                       aTGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTc                                                                                                           >  2:1180589/1‑140 (MQ=255)                       aTGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTc                                                                                                           >  2:1142528/1‑140 (MQ=255)                        tgctgcCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTcc                                                                                                          >  1:841098/1‑140 (MQ=255)                          ctgcCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAg                                                                                                        <  2:1179772/140‑1 (MQ=255)                          ctgcCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAg                                                                                                        <  1:637540/140‑1 (MQ=255)                          ctgcCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAg                                                                                                        <  1:1180589/140‑1 (MQ=255)                          ctgcCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAg                                                                                                        <  1:1131654/140‑1 (MQ=255)                             ccATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTc                                                                                                     <  1:576455/140‑1 (MQ=255)                               aTCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTcgc                                                                                                   >  1:41124/1‑140 (MQ=255)                                  ggCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTa                                                                                                <  1:209789/140‑1 (MQ=255)                                  ggCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCACACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTa                                                                                                >  2:1217525/1‑140 (MQ=255)                                                tgtAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAAc                                                                                  >  2:1023182/1‑140 (MQ=255)                                                  tAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATCCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGt                                                                                >  1:525742/1‑140 (MQ=255)                                                     ttcagttcCATCAGTGCAGAATGCCCGGTTCCGGCGTTATCCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGgc                                                                             <  2:525742/140‑1 (MQ=255)                                                          ttcCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGC‑ACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCAt                                                                       >  1:214344/1‑140 (MQ=255)                                                          ttcCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTACGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCa                                                                        >  1:1148252/1‑140 (MQ=255)                                                               tCAGTGTAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATg                                                                   >  2:1254125/1‑140 (MQ=255)                                                                   tGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATgccgc                                                               <  2:41124/140‑1 (MQ=255)                                                                     cAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATgccgccg                                                             >  2:538660/1‑140 (MQ=255)                                                                       gAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGC‑ACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCa                                                          <  2:214344/140‑1 (MQ=255)                                                                        aaTGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCa                                                          >  1:1020110/1‑140 (MQ=255)                                                                          tGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAAt                                                        >  2:441847/1‑140 (MQ=255)                                                                          tGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAAt                                                        <  1:479663/140‑1 (MQ=255)                                                                          tGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAAt                                                        <  1:300794/140‑1 (MQ=255)                                                                             ccGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCaa                                                     <  1:441847/140‑1 (MQ=255)                                                                             ccGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCACACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCaa                                                     <  1:1217525/140‑1 (MQ=255)                                                                                gTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAg                                                  <  2:1020110/140‑1 (MQ=255)                                                                                  tCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTa                                                >  2:987014/1‑140 (MQ=255)                                                                                  tCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTa                                                >  1:1056565/1‑140 (MQ=255)                                                                                     ggCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACAt                                             <  1:538660/140‑1 (MQ=255)                                                                                                 g‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGct                                 >  2:655086/1‑140 (MQ=255)                                                                                                    cGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGctcc                               >  1:1252334/1‑140 (MQ=255)                                                                                                       tCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCg                            >  2:341497/1‑140 (MQ=255)                                                                                                       tCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCg                            >  1:570934/1‑140 (MQ=255)                                                                                                       tCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCg                            >  2:536906/1‑140 (MQ=255)                                                                                                       tCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCg                            <  2:841098/140‑1 (MQ=255)                                                                                                       tCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTACAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCg                            >  1:121092/1‑140 (MQ=255)                                                                                                                    cgcgACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGAcgctttcgctt               >  1:256419/1‑140 (MQ=255)                                                                                                                    cgcgACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCC‑CGGAcgctttcgcttt              >  2:823390/1‑140 (MQ=255)                                                                                                                       gACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTg            >  1:858228/1‑140 (MQ=255)                                                                                                                         cacCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGa                          <  1:812793/124‑1 (MQ=255)                                                                                                                         cacCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGa                          >  2:812793/1‑124 (MQ=255)                                                                                                                                 aGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCGCCATCGa  >  2:62184/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      GGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCTCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCGCCATCGA  >  NC_000913/2306434‑2306700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑