Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,319,804	G→A	L25F (CTC→TTC)	rcsC ←	sensory histidine kinase RcsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,319,804	0	G	A	100.0%	294.8 / NA	83	L25F (CTC→TTC)	rcsC	sensory histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/37);  total (46/37)

CACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGTTAAGGTAGCGGTAAAAGCGTGTTA  >  NC_000913/2319670‑2319940                                                                                                                                       |                                                                                                                                         cacatcaGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTa                                                                                                                                     >  2:945174/1‑140 (MQ=255)  acatcaGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTaa                                                                                                                                    <  1:973232/140‑1 (MQ=255)    atcaGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACt                                                                                                                                      >  2:95347/1‑136 (MQ=255)    atcaGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACt                                                                                                                                      <  1:95347/136‑1 (MQ=255)    atcaGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACg                                                                                                                                  >  2:397516/1‑140 (MQ=255)    atcaGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACg                                                                                                                                  >  2:1000230/1‑140 (MQ=255)     tcaGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGc                                                                                                                                 >  2:580298/1‑140 (MQ=255)     tcaGAGGCGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGc                                                                                                                                 >  1:942865/1‑140 (MQ=255)       agagGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACt                                                                                                                                      >  2:882627/1‑133 (MQ=255)       agagGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACt                                                                                                                                      <  1:882627/133‑1 (MQ=255)       agagGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCa                                                                                                                               >  2:566137/1‑140 (MQ=255)       agagGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCa                                                                                                                               <  1:192321/140‑1 (MQ=255)         agGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAAt                                                                                                                             <  2:222543/140‑1 (MQ=255)         agGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAAt                                                                                                                             >  1:1069020/1‑140 (MQ=255)          ggTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATg                                                                                                                            <  1:680078/140‑1 (MQ=255)           gTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGc                                                                                                                           <  2:991929/140‑1 (MQ=255)              cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCaa                                                                                                                              <  2:277833/134‑1 (MQ=255)              cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCaa                                                                                                                              >  1:277833/1‑134 (MQ=255)              cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGctc                                                                                                                         >  2:689081/1‑139 (MQ=255)              cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGctc                                                                                                                         <  1:689081/139‑1 (MQ=255)              cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGc                                                                                                                           >  2:1048935/1‑137 (MQ=255)              cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGc                                                                                                                           <  1:1048935/137‑1 (MQ=255)                ttGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGa                                                                                                                      >  1:347524/1‑140 (MQ=255)                ttGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGa                                                                                                                      >  2:568467/1‑140 (MQ=255)                          cgcTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAgcg                                                                                                            >  2:1128550/1‑140 (MQ=255)                             tGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGa                                                                                                         >  1:913799/1‑140 (MQ=255)                              gAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAg                                                                                                        <  1:397516/140‑1 (MQ=255)                              gAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAg                                                                                                        <  2:315029/140‑1 (MQ=255)                               aGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAgg                                                                                                       >  1:453146/1‑140 (MQ=255)                                   tGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCttt                                                                                                   <  2:347524/140‑1 (MQ=255)                                         gAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGa                                                                                                         >  1:663798/1‑128 (MQ=255)                                         gAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGa                                                                                                         <  2:663798/128‑1 (MQ=255)                                         gAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGt                                                                                             >  1:945768/1‑140 (MQ=255)                                           aCTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTg                                                                                           >  2:1060148/1‑140 (MQ=255)                                                 aTTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGaa                                                                                     >  2:811386/1‑140 (MQ=255)                                                 aTTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGaa                                                                                     >  2:146861/1‑140 (MQ=255)                                                    aaaTTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAaag                                                                                  <  2:50404/140‑1 (MQ=255)                                                      aTTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAaagaa                                                                                >  2:1080782/1‑140 (MQ=255)                                                      aTTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGACGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAaagaa                                                                                >  2:1231184/1‑140 (MQ=255)                                                       ttCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAaagaag                                                                               <  2:913799/140‑1 (MQ=255)                                                       ttCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAaagaag                                                                               <  1:146861/140‑1 (MQ=255)                                                         ccTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCa                                                                             <  2:942865/140‑1 (MQ=255)                                                         ccTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCa                                                                             >  1:699666/1‑140 (MQ=255)                                                             aCGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGt                                                                         >  1:539636/1‑140 (MQ=255)                                                              cGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTa                                                                        <  2:1020132/140‑1 (MQ=255)                                                                 aTTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAgg                                                                          <  2:218785/135‑1 (MQ=255)                                                                 aTTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAgg                                                                          >  1:218785/1‑135 (MQ=255)                                                                             cGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGaa                                                         >  2:629348/1‑140 (MQ=255)                                                                               cTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGc                                                       <  1:629348/140‑1 (MQ=255)                                                                                       aCGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTc                                               >  1:593360/1‑140 (MQ=255)                                                                                        cGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCa                                              >  2:1250824/1‑140 (MQ=255)                                                                                            ttAACGATGTAAAAAGCGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTg                                          >  1:124580/1‑140 (MQ=255)                                                                                             tAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGa                                         >  2:686168/1‑140 (MQ=255)                                                                                                 gATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGa                                     <  1:1060148/140‑1 (MQ=255)                                                                                                   tGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATg                                   >  1:905893/1‑140 (MQ=255)                                                                                                   tGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATg                                   >  1:893874/1‑140 (MQ=255)                                                                                                   tGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATg                                   <  1:686168/140‑1 (MQ=255)                                                                                                   tGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATg                                   >  1:168475/1‑140 (MQ=255)                                                                                                   tGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATg                                   <  1:168253/140‑1 (MQ=255)                                                                                                    gTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATgg                                  >  2:595165/1‑140 (MQ=255)                                                                                                     tAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGa                                 >  2:925658/1‑140 (MQ=255)                                                                                                     tAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGa                                 <  1:580298/140‑1 (MQ=255)                                                                                                     tAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGa                                 <  1:1175544/140‑1 (MQ=255)                                                                                                     tAAAAAACGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGa                                 >  1:1098864/1‑140 (MQ=255)                                                                                                      aaaaaaCGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAg                                <  1:349235/140‑1 (MQ=255)                                                                                                       aaaaaCGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAgt                               <  1:811386/140‑1 (MQ=255)                                                                                                       aaaaaCGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAgt                               <  2:539636/140‑1 (MQ=255)                                                                                                            cGGATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGt                          <  2:1028932/140‑1 (MQ=255)                                                                                                             ggATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGtt                         >  1:1014247/1‑140 (MQ=255)                                                                                                             ggATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGtt                         >  2:811859/1‑140 (MQ=255)                                                                                                             ggATGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGtt                         >  1:36973/1‑140 (MQ=255)                                                                                                                tGAAAAAGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCTAGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGTTAAg                      >  2:861330/1‑140 (MQ=255)                                                                                                                  aaaaaGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGTTAAGGt                    <  1:996792/140‑1 (MQ=255)                                                                                                                  aaaaaGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGTTAAGGt                    <  1:595165/140‑1 (MQ=255)                                                                                                                    aaaGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGTTAAGGTAg                  <  1:925658/140‑1 (MQ=255)                                                                                                                    aaaGCAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGTTAAGGTAg                  <  1:1080782/140‑1 (MQ=255)                                                                                                                        cAATCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATCGAGTGTGGTTAAGGTAGCGGt              <  2:893874/140‑1 (MQ=255)                                                                                                                         aaTCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGTTAAGGTAGCGGTa             >  2:1099570/1‑140 (MQ=255)                                                                                                                         aaTCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGTTAAGGTAGCGGTa             >  2:843379/1‑140 (MQ=255)                                                                                                                          aTCAACAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGTTAAGGTAGCGGTaa            <  2:699666/140‑1 (MQ=255)                                                                                                                               cAGCCAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGTTAAGGTAGCGGTAAAAGCg       >  1:1011343/1‑140 (MQ=255)                                                                                                                                  ccAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGTTAAGGTAGCGGTAAAAGCgtgt    <  2:593360/140‑1 (MQ=255)                                                                                                                                  ccAGAACACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGTTAAGGTAGCGGTAAAAGCgtgt    >  1:276765/1‑140 (MQ=255)                                                                                                                                    agaaCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGTTAAGGTAGCGGTAAAAGCGTGTTa  <  1:861330/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAGAAGCAAGGTATTTCAAAGGGGCGAAGCTCCGCCTCAGGTGACCGATGGAGTGTGGTTAAGGTAGCGGTAAAAGCGTGTTA  >  NC_000913/2319670‑2319940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑