Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,355,297	T→C	Y345H (TAT→CAT)	glpB →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,355,297	0	T	C	100.0%	191.7 / NA	55	Y345H (TAT→CAT)	glpB	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/25);  total (30/25)

CGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGA  >  NC_000913/2355166‑2355430                                                                                                                                    |                                                                                                                                      cGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGga                                                                                                                               >  2:92253/1‑140 (MQ=255) cGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGga                                                                                                                               >  1:472868/1‑140 (MQ=255)   cTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGGCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGgaga                                                                                                                             >  2:1141497/1‑140 (MQ=255)        tCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGAttttt                                                                                                                        >  1:479371/1‑140 (MQ=255)         ccACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGAtttttt                                                                                                                       <  2:696237/140‑1 (MQ=255)               ttCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGcgcc                                                                                                                 <  1:600684/140‑1 (MQ=255)                  gcgGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCa                                                                                                              <  1:92253/140‑1 (MQ=255)                  gcgGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCa                                                                                                              <  2:464103/140‑1 (MQ=255)                         tcGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCGACCGTgg                                                                                                       >  1:639399/1‑140 (MQ=255)                         tcGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTgg                                                                                                       >  2:23252/1‑140 (MQ=255)                           gCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGgca                                                                                                     <  2:216574/140‑1 (MQ=255)                           gCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGgca                                                                                                     >  1:971648/1‑140 (MQ=255)                            ccAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGgcag                                                                                                    <  1:213527/140‑1 (MQ=255)                                cGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGcgcc                                                                                                                 >  1:101018/1‑123 (MQ=255)                                cGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGcgcc                                                                                                                 <  2:101018/123‑1 (MQ=255)                                cGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGt                                                                                                >  2:278722/1‑140 (MQ=255)                                cGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGt                                                                                                >  1:142387/1‑140 (MQ=255)                                     gTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGgt                                                                                           <  1:278722/140‑1 (MQ=255)                                       ttCTTTAGTGGCGGGCTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGgtgt                                                                                         <  1:327995/140‑1 (MQ=255)                                          tttAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAAc                                                                                      <  2:1097061/140‑1 (MQ=255)                                          tttAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTAGCAGCAGTTCGGTGTAAc                                                                                      <  1:368995/140‑1 (MQ=255)                                                ggCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACtga                                                                                >  2:401755/1‑140 (MQ=255)                                                 gCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACtgat                                                                               >  2:509729/1‑140 (MQ=255)                                                  cGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACtgatg                                                                              >  2:1202186/1‑140 (MQ=255)                                                  cGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACtgatg                                                                              >  2:377186/1‑140 (MQ=255)                                                   ggACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGgt                                                                                           <  1:130728/126‑1 (MQ=255)                                                   ggACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGgt                                                                                           >  2:130728/1‑126 (MQ=255)                                                   ggACTGGTAGCGGAACGTAACGACATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACtgatga                                                                             >  2:798679/1‑140 (MQ=255)                                                     aCTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATgaga                                                                           <  2:639399/140‑1 (MQ=255)                                                       tGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACg                                                                         >  1:822476/1‑140 (MQ=255)                                                          tAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTa                                                                      <  1:35561/140‑1 (MQ=255)                                                             cGGAACGTAACGACATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGc                                                                   <  1:798679/140‑1 (MQ=255)                                                               gAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGccc                                                                 >  1:1211967/1‑140 (MQ=255)                                                               gAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGccc                                                                 >  1:164979/1‑140 (MQ=255)                                                                    tAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTcac                                                            >  1:807941/1‑140 (MQ=255)                                                                       cGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCAcagg                                                         <  2:142387/140‑1 (MQ=255)                                                                            ttCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAggg                                                    >  2:565511/1‑140 (MQ=255)                                                                              cGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCa                                                  <  1:401755/140‑1 (MQ=255)                                                                                agagCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCaaa                                                <  1:377186/140‑1 (MQ=255)                                                                                       aTTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGa                                         >  2:1249698/1‑140 (MQ=255)                                                                                       aTTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGa                                         >  2:462791/1‑140 (MQ=255)                                                                                       aTTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGa                                         >  2:119519/1‑140 (MQ=255)                                                                                                           aCAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAtcggtt                     <  2:164979/140‑1 (MQ=255)                                                                                                           aCAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAtcggtt                     <  1:565511/140‑1 (MQ=255)                                                                                                           aCAAACCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAtcggtt                     <  1:1141497/140‑1 (MQ=255)                                                                                                             aaaCCGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAtcggttcg                   >  1:65374/1‑140 (MQ=255)                                                                                                                 cGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGc               >  2:832020/1‑140 (MQ=255)                                                                                                                 cGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGc               >  2:885852/1‑140 (MQ=255)                                                                                                                 cGCCACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGc               >  2:975165/1‑140 (MQ=255)                                                                                                                  gCCACGCGGGGTGAATGGCATAAGGAAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCt              <  1:344239/140‑1 (MQ=255)                                                                                                                    cACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTG‑AAACCTGTTTGCCAtcggtt                     >  1:808872/1‑130 (MQ=255)                                                                                                                    cACGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTG‑AAACCTGTTTGCCAtcggtt                     <  2:808872/130‑1 (MQ=255)                                                                                                                     aCGCGGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTggg           <  1:832020/140‑1 (MQ=255)                                                                                                                      cgcgGGGTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGc          >  1:1023719/1‑140 (MQ=255)                                                                                                                           ggTGAATGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGAtt     >  1:1226000/1‑140 (MQ=255)                                                                                                                                 tGGCATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGa  <  1:543200/137‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      CGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGA  >  NC_000913/2355166‑2355430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑