Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,356,131	T→C	V204A (GTA→GCA)	glpC →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,356,131	0	T	C	100.0%	272.8 / NA	77	V204A (GTA→GCA)	glpC	anaerobic glycerol‑3‑phosphate dehydrogenase subunit C
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/33);  total (44/33)

ATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCG  >  NC_000913/2355996‑2356266                                                                                                                                        |                                                                                                                                        ataAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCg                                                                                                                                     >  2:844112/1‑140 (MQ=255)     aGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTaa                                                                                                                                 <  2:897809/140‑1 (MQ=255)     aGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTaa                                                                                                                                 >  2:22080/1‑140 (MQ=255)         cAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGc                                                                                                                             >  2:917551/1‑140 (MQ=255)          aGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGcc                                                                                                                            >  1:660327/1‑140 (MQ=255)          aGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGcc                                                                                                                            >  2:220941/1‑140 (MQ=255)          aGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGcc                                                                                                                            >  1:92897/1‑140 (MQ=255)            gTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCaa                                                                                                                          >  2:406945/1‑140 (MQ=255)            gTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCaa                                                                                                                          >  2:509273/1‑140 (MQ=255)                 tttctttcACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCt                                                                                                                     >  2:787894/1‑140 (MQ=255)                  ttctttcACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCtt                                                                                                                    <  2:624470/140‑1 (MQ=255)                               gctTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCa                                                                                                       <  1:496919/140‑1 (MQ=255)                                             aaCCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAAt                                                                                         >  1:746682/1‑140 (MQ=255)                                             aaCCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAAt                                                                                         >  1:1195342/1‑140 (MQ=255)                                                cATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTAc                                                                                      <  1:917551/140‑1 (MQ=255)                                                cATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTAc                                                                                      <  1:535353/140‑1 (MQ=255)                                                cATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTAc                                                                                      <  1:528375/140‑1 (MQ=255)                                                cATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTAc                                                                                      <  2:1252830/140‑1 (MQ=255)                                                  tCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGa                                                                                    >  2:122319/1‑140 (MQ=255)                                                  tCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGa                                                                                    >  2:574331/1‑140 (MQ=255)                                                   ccGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGaa                                                                                   >  2:1143300/1‑140 (MQ=255)                                                     gCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATg                                                                                 <  2:746682/140‑1 (MQ=255)                                                      cAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCa                                                                                           <  2:251230/129‑1 (MQ=255)                                                      cAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCa                                                                                           >  1:251230/1‑129 (MQ=255)                                                           aGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGt                                                                           <  1:574331/140‑1 (MQ=255)                                                               aaaGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGAt                                                                       <  1:844112/140‑1 (MQ=255)                                                                     ttaattaaAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGa                                                                 >  1:1139678/1‑140 (MQ=255)                                                                     ttaattaaAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGa                                                                 <  2:660327/140‑1 (MQ=255)                                                                       aattaaAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAGATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAg                                                               >  2:583595/1‑140 (MQ=255)                                                                       aattaaAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAg                                                               >  1:276440/1‑140 (MQ=255)                                                                            aaGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCtgt                                                           >  2:355874/1‑139 (MQ=255)                                                                            aaGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCtgt                                                           <  1:355874/139‑1 (MQ=255)                                                                             aGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATTCGCGAAGCTGTgg                                                         <  1:509273/140‑1 (MQ=255)                                                                             aGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTgg                                                         >  1:468976/1‑140 (MQ=255)                                                                             aGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTgg                                                         <  1:406945/140‑1 (MQ=255)                                                                             aGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTgg                                                         <  2:468976/140‑1 (MQ=255)                                                                                 cTCAACGCAATGGGTACCGGTTTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGt                                                     >  2:282105/1‑140 (MQ=255)                                                                                 cTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGt                                                     >  1:169742/1‑140 (MQ=255)                                                                                 cTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGt                                                     >  2:222931/1‑140 (MQ=255)                                                                                      cGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAg                                                >  1:813843/1‑140 (MQ=255)                                                                                      cGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAg                                                >  2:157075/1‑140 (MQ=255)                                                                                       gCAATGGGTACCGGTTTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAgg                                               <  1:282105/140‑1 (MQ=255)                                                                                           tGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCAtt                                           >  2:396820/1‑140 (MQ=255)                                                                                            gggTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTc                                          <  2:35903/140‑1 (MQ=255)                                                                                              gTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCg                                        <  2:946885/140‑1 (MQ=255)                                                                                              gTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCg                                        >  2:300692/1‑140 (MQ=255)                                                                                                       tACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGcc                               <  1:157075/140‑1 (MQ=255)                                                                                                       tACAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGcc                               >  1:565279/1‑140 (MQ=255)                                                                                                        aCAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGGGTAAAAGGCATTCCGGTGATTGCCa                              <  1:22080/140‑1 (MQ=255)                                                                                                        aCAACTGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCa                              <  1:300692/140‑1 (MQ=255)                                                                                                             tGCTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCAcctcc                         <  1:396820/140‑1 (MQ=255)                                                                                                              gCTCAGCAAAGAAAGATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCAcctcct                        <  1:583595/140‑1 (MQ=255)                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCa                              <  2:1228081/133‑1 (MQ=255)                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCa                              >  2:905437/1‑133 (MQ=255)                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCa                              >  1:1228081/1‑133 (MQ=255)                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCa                              <  1:905437/133‑1 (MQ=255)                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACctcctc                       >  2:1260704/1‑140 (MQ=255)                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACctcctc                       >  2:553337/1‑140 (MQ=255)                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACctcctc                       >  2:429363/1‑140 (MQ=255)                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACctcctc                       >  1:805146/1‑140 (MQ=255)                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACctcctc                       >  2:1079050/1‑140 (MQ=255)                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCACTCCGGTGATTGCCACctcctc                       >  2:677348/1‑140 (MQ=255)                                                                                                                tCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCa                      <  1:222931/140‑1 (MQ=255)                                                                                                                tCAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCa                      <  1:118693/140‑1 (MQ=255)                                                                                                                 cAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCaa                     >  2:30652/1‑140 (MQ=255)                                                                                                                 cAGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCaa                     >  1:1018284/1‑140 (MQ=255)                                                                                                                  aGCAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAAc                    <  2:1195342/140‑1 (MQ=255)                                                                                                                    cAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCt                  >  1:1002275/1‑140 (MQ=255)                                                                                                                    cAAAGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCt                  >  1:1064833/1‑140 (MQ=255)                                                                                                                     aaaGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTg                 <  2:1153301/140‑1 (MQ=255)                                                                                                                     aaaGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTg                 <  1:553337/140‑1 (MQ=255)                                                                                                                       aGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTa               >  2:1143499/1‑140 (MQ=255)                                                                                                                       aGAAAAATGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTa               >  1:321983/1‑140 (MQ=255)                                                                                                                         aaaaaTGCTGCGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACa             >  1:812450/1‑140 (MQ=255)                                                                                                                               gctgcGGCGCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGcc       <  1:1143300/140‑1 (MQ=255)                                                                                                                                   cggcgCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTgc   >  1:129768/1‑140 (MQ=255)                                                                                                                                    ggcgCACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTgcg  <  2:129768/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCG  >  NC_000913/2355996‑2356266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑